Antibody dependent cell mediated cytotoxicity and erythrophagocytosis assays in Rh haemolytic disease of the newborn.

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dc.contributor.authorPatil, J Sen_US
dc.contributor.authorGupte, S Cen_US
dc.date.accessioned2009-05-28T05:08:28Z
dc.date.available2009-05-28T05:08:28Z
dc.date.issued1996-06-01en_US
dc.descriptionThe Ceylon Medical Journal.en_US
dc.description.abstractOBJECTIVE: To evaluate the role of antibody dependent cell mediated cytotoxicity (ADCC) and erythrophagocytosis in comparison to IgG subtypes and concentration of anti-D in haemolytic disease of the newborn (HDN). DESIGN, SETTING AND PATIENTS: One hundred Rh (D) immunised women attending an antenatal clinic of Nowrosjee Wadia Maternity Hospital at 32 to 34 weeks of gestation. Results of 90 women having Rh (D) positive infants were correlated with severity based on outcome of pregnancy, cord blood Hb and treatment given to infant. MEASUREMENTS: Immunoradiometric assay (IRMA) was used for anti-D quantitation. In ADCC assay 51Cr release in the culture supernatant was measured, and in the phagocytosis assay lytic activity was measured on spectrophotometer. Cord blood monocytes were used as effector cells in both the assays. RESULTS: Good correlation (P < 0.01) was observed between ADCC vs IRMA and ADCC vs phagocytosis. ADCC% specific lysis was significantly higher and still-birth rate was increased when mothers had IgG1 + IgG3 type of anti-D. Though all variables showed significant correlation (P < 0.01) with severity, ADCC assay was the most predictive. Cord blood haemoglobin showed a significant inverse correlation with ADCC and phagocytosis assays. CONCLUSION: In the absence of a cordocentesis facility severity of Rh HDN could be reliably judged by ADCC assay. If an isotope laboratory is not available, then instead of ADCC, phagocytosis assay may be employed.en_US
dc.description.affiliationInstitute of Immunohaematology (ICMR), KEM Hospital Campus, Parel, Bombay.en_US
dc.identifier.citationPatil JS, Gupte SC. Antibody dependent cell mediated cytotoxicity and erythrophagocytosis assays in Rh haemolytic disease of the newborn. The Ceylon Medical Journal. 1996 Jun; 41(2): 46-50en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/47999
dc.language.isoengen_US
dc.source.urihttps://www.infolanka.com/CMJhome/en_US
dc.subject.meshCytotoxicity Tests, Immunologicen_US
dc.subject.meshCytotoxicity, Immunologicen_US
dc.subject.meshErythroblastosis, Fetal --immunologyen_US
dc.subject.meshErythrocytes --immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoradiometric Assayen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshPhagocytosisen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshPregnancyen_US
dc.titleAntibody dependent cell mediated cytotoxicity and erythrophagocytosis assays in Rh haemolytic disease of the newborn.en_US
dc.typeJournal Articleen_US
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