Kinetics of viral RNA, immunoglobulin-M & G antibodies in Kyasanur forest disease

Simple item page
dc.contributor.authorYadav, PDen_US
dc.contributor.authorGurav, YKen_US
dc.contributor.authorShete, AMen_US
dc.contributor.authorJain, Ren_US
dc.contributor.authorNyayanit, DAen_US
dc.contributor.authorPardeshi, PGen_US
dc.contributor.authorViswanathan, Ren_US
dc.contributor.authorChiplunkar, TRen_US
dc.contributor.authorAwate, Pen_US
dc.contributor.authorMajumdar, TPen_US
dc.contributor.authorSahay, RRen_US
dc.contributor.authorMourya, DTen_US
dc.date.accessioned2020-04-10T01:43:46Z
dc.date.available2020-04-10T01:43:46Z
dc.date.issued2019-08
dc.description.abstractBackground & objectives: Kyasanur forest disease (KFD) is an infectious disease discovered in Karnataka State of India in 1957; since then, the State has been known to be enzootic for KFD. In the last few years, its presence was observed in the adjoining five States of the Western Ghats of India. The present study was conducted to understand the kinetics of viral RNA, immunoglobulin M (IgM) and IgG antibody in KFD-infected humans for developing a diagnostic algorithm for KFD. Methods: A prospective follow up study was performed among KFD patients in Sindhudurg district of Maharashtra State, India. A total of 1046 suspected patients were tested, and 72 KFD patients were enrolled and followed for 17 months (January 2016 to May 2017). Serum samples of KFD patients were screened for viral RNA, and IgM and IgG antibodies. Results: KFD viral positivity was observed from 1st to 18th post-onset day (POD). Positivity of anti-KFD virus (KFDV) IgM antibodies was detected from 4th till 122nd POD and anti-KFDV IgG antibodies detected from 5th till 474th POD. A prediction probability was determined from statistical analysis using the generalized additive model in R-software to support the laboratory findings regarding viral kinetics. Interpretation & conclusions: This study demonstrated the presence of KFD viral RNA till 18th POD, IgM antibodies till 122nd POD and IgG till the last sample collected. Based on our study an algorithm was recommended for accurate laboratory diagnosis of KFDV infection. A sample collected between 1 and 3 POD can be tested using KFDV real-time reverse transcriptase polymerase chain reaction (RT-PCR); between 4 and 24 POD, the combination of real-time RT-PCR and anti-KFDV IgM enzyme-linked immunosorbent assay (ELISA) tests can be used; between POD 25 and 132, anti-KFDV IgM and IgG ELISA are recommended.en_US
dc.identifier.affiliationsMaximum Containment Laboratory, ICMR-National Institute of Virology, Pune, Maharashtra, Indiaen_US
dc.identifier.affiliationsEpidemiology Group, ICMR-National Institute of Virology, Pune, Maharashtra, Indiaen_US
dc.identifier.affiliationsBacteriology Group, ICMR-National Institute of Virology, Pune, Maharashtra, Indiaen_US
dc.identifier.affiliationsRural Hospital, Dodamarg, Sindhudurg, Maharashtra, Indiaen_US
dc.identifier.affiliationsState Public Health Epidemiology Department, Maharashtra, Indiaen_US
dc.identifier.affiliationsICMR-National Institute of Virology, Pune, Maharashtra, Indiaen_US
dc.identifier.citationYadav PD, Gurav YK, Shete AM, Jain R, Nyayanit DA, Pardeshi PG, Viswanathan R, Chiplunkar TR, Awate P, Majumdar TP, Sahay RR, Mourya DT. Kinetics of viral RNA, immunoglobulin-M & G antibodies in Kyasanur forest disease. Indian Journal of Medical Research. 2019 Aug; 150(2): 186-193en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195977
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber2en_US
dc.relation.volume150en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1929_17en_US
dc.subjectDiagnostic algorithmen_US
dc.subjecthumanen_US
dc.subjectIgM/IgG antibodyen_US
dc.subjectKyasanur forest diseaseen_US
dc.subjectMaharashtraen_US
dc.subjectviral RNAen_US
dc.titleKinetics of viral RNA, immunoglobulin-M & G antibodies in Kyasanur forest diseaseen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijmr2019v150n2p186.pdf
Size:
1.8 MB
Format:
Adobe Portable Document Format
Download
Collections
Indian Journal of Medical Research