Construction and characterization of an unmarked aroC deletion mutant of Burkholderia pseudomallei strain A2.
Loading...
Date
2009-01-28
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Using a sucrose counter-selection strategy, we have constructed an unmarked aroC deletion mutant of B. pseudomallei strain A2 in order to investigate the possibility of an effective live-attenuated vaccine. The aroC deletion in the resultant mutant, designated A2DeltaaroC, was confirmed by PCR, Southern hybridization and failure of the mutant to grow in a defined medium without aromatic compounds. Compared to the parental wild type strain, A2DeltaaroC was highly attenuated for virulence following intraperitoneal introduction into BALB/c and C57BL/6 mice. BALB/c mice immunized intraperitoneally with A2DeltaaroC were not protected against a challenge dose of 500 cfu (25 x LD50) of the parental strain A2, whereas C57BL/6 mice similarly immunized intraperitoneally with A2DeltaaroC were significantly protected against a challenge dose of 6,000 cfu (20 x LD50).
Description
The Southeast Asian Journal of Tropical Medicine and Public Health.
Keywords
Citation
Srilunchang T, Proungvitaya T, Wongratanacheewin S, Strugnell R, Homchampa P. Construction and characterization of an unmarked aroC deletion mutant of Burkholderia pseudomallei strain A2. The Southeast Asian Journal of Tropical Medicine and Public Health. 2009 Jan; 40(1): 123-30