Functional analyses of a flavonol synthase–like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation.

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dc.contributor.authorZhou, Xing-Wen
dc.contributor.authorFan, Zheng-Qi
dc.contributor.authorChen, Yue
dc.contributor.authorZhu, Yu-Lin
dc.contributor.authorLi, Ji-Yuan
dc.contributor.authorYin, Heng-Fu
dc.date.accessioned2015-08-13T10:44:14Z
dc.date.available2015-08-13T10:44:14Z
dc.date.issued2013-09
dc.description.abstractThe flavonoids metabolic pathway plays central roles in floral coloration, in which anthocyanins and flavonols are derived from common precursors, dihydroflavonols. Flavonol synthase (FLS) catalyses dihydroflavonols into flavonols, which presents a key branch of anthocyanins biosynthesis. The yellow flower of Camellia nitidissima Chi. is a unique feature within the genus Camellia, which makes it a precious resource for breeding yellow camellia varieties. In this work, we characterized the secondary metabolites of pigments during floral development of C. nitidissima and revealed that accumulation of flavonols correlates with floral coloration. We first isolated CnFLS1 and showed that it is a FLS of C. nitidissima by gene family analysis. Second, expression analysis during floral development and different floral organs indicated that the expression level of CnFLS1 was regulated by developmental cues, which was in agreement with the accumulating pattern of flavonols. Furthermore, over-expression of CnFLS1 in Nicotiana tabacum altered floral colour into white or light yellow, and metabolic analysis showed significant increasing of flavonols and reducing of anthocyanins in transgenic plants. Our work suggested CnFLS1 plays critical roles in yellow colour pigmentation and is potentially a key point of genetic engineering toward colour modification in Camellia.en_US
dc.identifier.citationZhou Xing-Wen, Fan Zheng-Qi, Chen Yue, Zhu Yu-Lin, Li Ji-Yuan, Yin Heng-Fu. Functional analyses of a flavonol synthase–like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation. Journal of Biosciences. 2013 Sept; 38(3): 593-604.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/161846
dc.language.isoenen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/sep2013/593.pdfen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/sep2013/supp/Zhou.pdfen_US
dc.subjectAnthocyaninen_US
dc.subjectCamelliaen_US
dc.subjectflavonoiden_US
dc.subjectflavonol synthaseen_US
dc.subjectflavonolen_US
dc.subjectpigmentationen_US
dc.titleFunctional analyses of a flavonol synthase–like gene from Camellia nitidissima reveal its roles in flavonoid metabolism during floral pigmentation.en_US
dc.typeArticleen_US
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