Cell cycle inhibitory activity of Piper longum against A549 cell line and its protective effect against metal-induced toxicity in rats.

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dc.contributor.authorSharma, Amit Kumar
dc.contributor.authorKumar, Shashank
dc.contributor.authorChashoo, Gousia
dc.contributor.authorSaxena, Ajit K
dc.contributor.authorPandey, Abhay K
dc.date.accessioned2014-12-29T06:51:19Z
dc.date.available2014-12-29T06:51:19Z
dc.date.issued2014-10
dc.description.abstractAnticancer potential of Piper longum fruit against human cancer cell lines (DU-145 prostate, A549 lung, THP-1 leukemia, IGR-OVI-1 ovary and MCF-7 breast) as well as its in vitro and in vivo biochemical efficacy in AlCl3-induced hepatotoxicity were evaluated in the rats. Dried samples were extracted with several solvents using soxhlet apparatus. Flavonoid content in chloroform, benzene, ethyl alcohol and aqueous extracts of fruit was 19, 14, 12 and 11 μg quercetin equivalent/mg of sample, respectively. Hexane extracts exhibited 90-92% cytotoxicity against most of the test cell lines (A549, THP-1, IGR-OVI-1 and MCF-7), while benzene extract displayed 84-87% cytotoxicity against MCF-7, IGR-OV-1 and THP-1 cell lines. Among extracts, hexane, benzene and acetone extracts demonstrated considerable cytotoxicity (91-95%) against A549 (lung cancer) cell line in Sulforhodamine B dye (SRB) assay. Cell cycle analysis revealed that hexane, benzene and acetone extracts produced 41, 63 and 43% sub-G1 DNA fraction, demonstrating cell cycle inhibitory potential of these extracts against A549 cell line. Chloroform, ethyl alcohol and aqueous extracts displayed 71, 64 and 65% membrane protective activity, respectively in lipid peroxidation inhibition assay. P. longum fruit extracts also ameliorated AlCl3-induced hepatotoxicity, as indicated by alterations observed in serum enzymes ALP, SGOT and SGPT activity, as well as creatinine and bilirubin contents. In conclusion, study established the cytotoxic and hepatoprotective activity in P. longum extracts.en_US
dc.identifier.citationSharma Amit Kumar, Kumar Shashank, Chashoo Gousia, Saxena Ajit K, Pandey Abhay K. Cell cycle inhibitory activity of Piper longum against A549 cell line and its protective effect against metal-induced toxicity in rats. Indian Journal of Biochemistry & Biophysics. 2014 Oct; 51(5): 358-364.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/154264
dc.publisherNISCAIR CSIR India.en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29884en_US
dc.subjectCytotoxicityen_US
dc.subjectCell cycleen_US
dc.subjectPiper longumen_US
dc.subjectLipid peroxidationen_US
dc.subjectHepatoprotectiveen_US
dc.subjectHepatotoxicityen_US
dc.subjectSerum enzymesen_US
dc.subject.meshAluminium Compounds
dc.subject.meshAnimals
dc.subject.meshAntineoplastic Agents --administration & dosage
dc.subject.meshApoptosis --drug efffects
dc.subject.meshCell Cycle Checkpoints --drug effects
dc.subject.meshCell Line, Tumor
dc.subject.meshChlorides
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshDrug-Induced Liver Injury --etiology
dc.subject.meshDrug-Induced Liver Injury --physiopathology
dc.subject.meshDrug-Induced Liver Injury --prevention & control
dc.subject.meshFruit --chemistry
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMetals
dc.subject.meshNeoplasms, Experimental --drug therapy
dc.subject.meshNeoplasms, Experimental --pathology
dc.subject.meshPiper --chemistry
dc.subject.meshPlant Extracts --administration & dosage
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshTreatment Outcome
dc.titleCell cycle inhibitory activity of Piper longum against A549 cell line and its protective effect against metal-induced toxicity in rats.en_US
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