Sequence analyses of three nuclear ribosomal loci and a mitochondrial locus in cytologically different forms of Thai Anopheles aconitus mosquitoes.

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dc.contributor.authorJariyapan, Narissaraen_US
dc.contributor.authorChoochote, Wejen_US
dc.contributor.authorJunkum, Anulucken_US
dc.contributor.authorJitpakdi, Atchariyaen_US
dc.contributor.authorKomalamisra, Narumonen_US
dc.contributor.authorBates, Paul Aen_US
dc.contributor.authorCrampton, Julian Men_US
dc.date.accessioned2009-05-27T14:49:34Z
dc.date.available2009-05-27T14:49:34Z
dc.date.issued2005-09-28en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractRibosomal DNA (rDNA) and mitochondrial DNA (mtDNA) of Anopheles aconitus mosquitoes were examined to investigate intra- and inter-species variation amongst the members of the Minimus group of Anopheles subgenus Cellia. Three rDNA loci (ITS1, ITS2 and D3 regions) and a mtDNA locus (cytochrome oxidase II) were analyzed in An. aconitus Form B and Form C collected in Chiang Mai Province, Thailand. The results show that the consensus sequences of the four loci of the two forms are consistent with those of mosquitoes in the genus Anopheles. No intraindividual variation was detected, but intrapopulation variation was present with polymorphic sequences in some forms for each gene examined. The variation rates were approximately 0.15 to 0.8%. These data indicate that An. aconitus Form B and Form C in Chiang Mai, Thailand are conspecific. In this study, the complete ITS1 sequence of An. aconitus is reported for the first time. The region showed a high variation rate (approximately 55%), compared to the closely related species An. minimus C. It is suggested that this rDNA locus may provide sequence information to differentiate the members of the Minimus group of Anopheles subgenus Cellia.en_US
dc.description.affiliationDepartment of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand. narsuwan@mail.med.cmu.ac.then_US
dc.identifier.citationJariyapan N, Choochote W, Junkum A, Jitpakdi A, Komalamisra N, Bates PA, Crampton JM. Sequence analyses of three nuclear ribosomal loci and a mitochondrial locus in cytologically different forms of Thai Anopheles aconitus mosquitoes. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 Sep; 36(5): 1162-73en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/31187
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2005_36_5/14-3499.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnopheles --classificationen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDNA, Mitochondrial --geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRNA, Ribosomal, 28S --geneticsen_US
dc.subject.meshSequence Analysis, DNAen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshThailanden_US
dc.titleSequence analyses of three nuclear ribosomal loci and a mitochondrial locus in cytologically different forms of Thai Anopheles aconitus mosquitoes.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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