IgG- and IgG4-detected antigens of Dirofilaria immitis adult worms for bancroftian filariasis by enzyme-linked immunoelectrotransfer blot.

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dc.contributor.authorDekumyoy, Pen_US
dc.contributor.authorInsun, Den_US
dc.contributor.authorWaikagul, Jen_US
dc.contributor.authorTanantaphruti, Men_US
dc.contributor.authorRongsriyam, Yen_US
dc.contributor.authorCoochote, Wen_US
dc.date.accessioned2009-05-27T16:50:18Z
dc.date.available2009-05-27T16:50:18Z
dc.date.issued2000-03-21en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractIn Thailand, Wuchereria bancrofti filariasis has persisted along the border between Thailand and Myanmar, its dynamic distribution caused by the infected transmigrants between neighboring countries, and the availability of susceptible mosquito vectors. Dirofilaria immitis adult worm was used as a source of antigens, excretory-secretory (ES) and partial surface extracts, to detect human filariasis. ES products showed several stained bands with Coomassie brilliant blue ranging from 14.5-93 kDa and mostly being glycoproteins as shown by concurrent reaction with Concanavalin A, except those at 18, 16 and 14.5 kDa which stained only with Coomassie brilliant blue. Surface proteins of 33.5-91.5 kDa were stained with Coomassie brilliant blue and showed smear bands with Concanavalin A. By enzyme-linked immunoelectrotransfer blot, Bancroftian filariasis sera gave specific reactions with glycoprotein ES antigens at MW 20.5 kDa against anti-human IgG. A prominent band of 18 kDa appeared consistently with the IgG4-ES antigen system. Surface extracts reacting with IgG and IgG4 were considered to be unsuitable as antibodies from all cases of filariasis could not detect any bands.en_US
dc.description.affiliationDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationDekumyoy P, Insun D, Waikagul J, Tanantaphruti M, Rongsriyam Y, Coochote W. IgG- and IgG4-detected antigens of Dirofilaria immitis adult worms for bancroftian filariasis by enzyme-linked immunoelectrotransfer blot. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 ; 31 Suppl 1(): 58-64en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36124
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Helminth --isolation & purificationen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshDirofilaria immitis --immunologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFemaleen_US
dc.subject.meshFilariasis --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshThailanden_US
dc.subject.meshWuchereria bancrofti --immunologyen_US
dc.titleIgG- and IgG4-detected antigens of Dirofilaria immitis adult worms for bancroftian filariasis by enzyme-linked immunoelectrotransfer blot.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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