Effect of sealing on the incorporation of pyrene in goat erythrocyte ghosts – Α fluorescence study.
| dc.contributor.author | Nandy, P | |
| dc.contributor.author | Choudhury, M Dutta | |
| dc.contributor.author | Chakrabarti, P | |
| dc.date.accessioned | 2015-07-21T07:28:19Z | |
| dc.date.available | 2015-07-21T07:28:19Z | |
| dc.date.issued | 1983-06 | |
| dc.description.abstract | The incorporation of pyrene within the membrane interior of goat erythrocyte ghost has been estimated from its fluorescence spectrum. The excimer to monomer fluorescence intensity ratio of embedded pyrene is a function of the fluidity of its environment and the magnitude of its incorporation. Our study shows that this ratio is considerably less (30%) in a pre-sealed ghost than in the non-sealed ghost revealing that the site of incorporation of the probe is indeed the hydrophobic interior of the membrane; as in the later case, the probe has access to the membrane interior from both sides of the membrane. Our study on kinetics of molecular exchange indicates a very fast (of the order of seconds) transfer rate of pyrene from probed to unprobed erythrocyte ghosts through the aqueous phase rather than actual fusion of the membranes. | en_US |
| dc.identifier.citation | Nandy P, Choudhury M Dutta, Chakrabarti P. Effect of sealing on the incorporation of pyrene in goat erythrocyte ghosts – Α fluorescence study. Journal of Biosciences. 1983 Jun; 5(2): 163-166. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/160225 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ias.ac.in/jarch/jbiosci/5/163-166.pdf | en_US |
| dc.subject | Erythrocyte ghosts | en_US |
| dc.subject | probe incorporation | en_US |
| dc.subject | excimer fluorescence | en_US |
| dc.title | Effect of sealing on the incorporation of pyrene in goat erythrocyte ghosts – Α fluorescence study. | en_US |
| dc.type | Article | en_US |