Production of surfactin from novel Bacillus spp. isolated from soil and its antifungal properties

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dc.contributor.authorAbirami, N.en_US
dc.contributor.authorJesteena, J.en_US
dc.contributor.authorRagunathan, R.en_US
dc.date.accessioned2024-11-30T11:59:22Z
dc.date.available2024-11-30T11:59:22Z
dc.date.issued2023-09
dc.description.abstractAim: This research aimed to screen the biosurfactant-producing bacteria from soil, especially for the betterment of the environment in terms of bioremediation and biodegradation of lipids and hydrocarbons. Methodology: Bacillus spp. isolated from the soil was cultured and inoculated into different mineral media such as Glucose, Sawdust, and Bagasse mineral media. The surfactin was extracted and the activity was studied using oil emulsification test, oil spreading assay, and drop collapse assay. Bacillus spp. that provided better results were sequenced and submitted to NCBI. The surfactin of the specific bacteria was characterized using TLC, UV-vis spectra, and FTIR analysis. Finally, the antifungal activity assay was performed against Aspergillus flavus, Rhizopus spp., and Aspergillus niger. Results: Bacillus species subjected to gene sequencing was identified as Bacillus mycoids strain ARJCBNR (On464184). UV-vis spectra and FTIR analyses revealed various functional groups such as 3356.14 cm-1 (N-H stretch), 1635.64 cm-1 (C=O amide), and 1211 cm-1 (C-O stretch) representing the surfactin compound between 4000 and 500 cm-1. Upon antifungal activity, sawdust-extracted surfactin had a higher zone of inhibition against all the tested fungi. Interpretation: This study revealed that Bacillus spp. can utilize agro-industrial waste as substrate to produce surfactin. Also, the surfactin extracted from sawdust mineral media showed higher antifungal activity.en_US
dc.identifier.affiliationsDepartment of Microbiology, Centre for Bioscience and Nanoscience Research, Coimbatore–641 021, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology, Centre for Bioscience and Nanoscience Research, Coimbatore–641 021, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Centre for Bioscience and Nanoscience Research, Coimbatore–641 021, Indiaen_US
dc.identifier.citationAbirami N., Jesteena J., Ragunathan R.. Production of surfactin from novel Bacillus spp. isolated from soil and its antifungal properties. Journal of Environmental Biology. 2023 Sept; 44(5): 728-735en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/238470
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber5en_US
dc.relation.volume44en_US
dc.source.urihttps://doi.org/10.22438/jeb/44/5/MRN-5103en_US
dc.subjectBacillus spp.en_US
dc.subjectBiosurfactanten_US
dc.subjectMineral mediaen_US
dc.subjectSoilen_US
dc.subjectSurfactinen_US
dc.titleProduction of surfactin from novel Bacillus spp. isolated from soil and its antifungal propertiesen_US
dc.typeJournal Articleen_US
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