A homologue of the defender against the apoptotic death gene (dad1 )in UV-exposed Chlamydomonas cells is downregulated with the onset of programmed cell death.

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dc.contributor.authorMoharikar, Swatien_US
dc.contributor.authorD'Souza, Jacinta Sen_US
dc.contributor.authorRao, Basuthkar Jen_US
dc.date.accessioned2007-03-17en_US
dc.date.accessioned2009-06-01T14:19:19Z
dc.date.available2007-03-17en_US
dc.date.available2009-06-01T14:19:19Z
dc.date.issued2007-03-17en_US
dc.description.abstractWe report here the isolation of a homologue of the potential anti-apoptotic gene, defender against apoptotic death (dad1 )from Chlamydomonas reinhardtii cells.Using polymerase chain reaction (PCR),we investigated its expression in the execution process of programmed cell death (PCD)in UV-C exposed dying C.reinhardtii cells.Reverse- transcriptase (RT)-PCR showed that C.reinhardtii dad1 amplification was drastically reduced in UV-C exposed dying C.reinhardtii cells.We connect the downregulation of dad1 with the upregulation of apoptosis protease activating factor-1 (APAF-1)and the physiological changes that occur in C.reinhardtii cells upon exposure to 12 J/m 2 UV-C in order to show a reciprocal relationship between proapoptotic and inhibitor of apoptosis factors.The temporal changes indicate a correlation between the onset of cell death and dad1 downregulation.The sequence of the PCR product of the cDNA encoding the dad1 homologue was aligned with the annotated dad1 (C_20215)from the Chlamydomonas database (https://genome.jgi-psf.org:8080/annotator/servlet/jgi.annotation.Annotation?pDb=chlre2); Annotation?pDb=chlre2 );this sequence was found to show 100% identity,both at the nucleotide and amino acid level. The 327 bp transcript showed an open reading frame of 87 amino acid residues.The deduced amino acid sequence of the putative C.reinhardtii DAD1 homologue showed 54% identity with Oryza sativa, 56 identity with Drosophila melanogaster, 66% identity with Xenopus laevis, and 64% identity with Homo sapiens,Sus scrofa,Gallus gallus,Rattus norvegicus and Mus musculus.en_US
dc.description.affiliationDepartment of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005.en_US
dc.identifier.citationMoharikar S, D'Souza JS, Rao BJ. A homologue of the defender against the apoptotic death gene (dad1 )in UV-exposed Chlamydomonas cells is downregulated with the onset of programmed cell death. Journal of Biosciences. 2007 Mar; 32(2): 261-70en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110746
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis --radiation effectsen_US
dc.subject.meshApoptosis Regulatory Proteins --metabolismen_US
dc.subject.meshApoptotic Protease-Activating Factor 1 --metabolismen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshChlamydomonas reinhardtii --geneticsen_US
dc.subject.meshDNA Primers --geneticsen_US
dc.subject.meshDown-Regulation --radiation effectsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Analysis, DNAen_US
dc.subject.meshUltraviolet Raysen_US
dc.titleA homologue of the defender against the apoptotic death gene (dad1 )in UV-exposed Chlamydomonas cells is downregulated with the onset of programmed cell death.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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