Physico-chemical characterisation and molecular organisation of the collagen from the skin of an air-breathing fish (Ophiocephalus striatus).
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Date
1983-06
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Abstract
Collagen has been prepared from the skin of an air-breathing Indian fish
(Ophiocephalus striatus) by extraction with cold 0.5M acetic acid and purification by alternate
precipitation with NaCl and dialysis against 0.02M Na2HPO4. The purified collagen was
characterised with respect to physico-chemical properties, amino acid composition and
chromatography of the denatured collagen. The fish collagen has a higher shrinkage
temperature and denaturation temperature compared to that of the allied teleosts living in
exclusively aquatic medium. These differences could possibly be reflections of the response to
the rigours of the environment. As found for other vertebrate collagens, the fish collagen
contains two kinds of single chains the α1 and α2 chains as revealed by sodium dodecyl
sulphate–polyacrylamidegel electrophoresis and carboxymethylcellulose chromatography.
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Keywords
Fish collagen, characterisation of collagen, chain composition
Citation
Rama S, Chandrakasan Gowri. Physico-chemical characterisation and molecular organisation of the collagen from the skin of an air-breathing fish (Ophiocephalus striatus). Journal of Biosciences. 1983 Jun; 5(2): 147-154.