Cytogenetic evaluation of cadmium chloride on Channa punctatus.
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Date
2012-05
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Abstract
The aim of present study was to evaluate the genotoxic effect of heavy metal in Channa punctatus through
the micronucleus test, chromosomal aberrations and sister chromatid exchange. The fish were kept separately
in 0.5, 1.0, 2.0 and 5.0 ppm cadmium chloride for 3 days. For micronucleus test blood was collected from
caudal vein and smeared on clean slides fixed in methanol and stained with 2% Giemsa. Mean frequency
of micronuclei observed was 0.10, 0.15, 0.24, 0.34 and 0.39 in control, 0.5, 1.0, 2.0 and 5.0 ppm CdCl2
respectively. In vivo chromosome preparation from kidney tissues was carried out. The mean frequency of
cells with aberrations observed was 0.13, 0.20, 0.34, 0.60 and 0.95 in control, 0.5, 1.0, 2.0 and 5.0 ppm
CdCl2 respectively. Likewise the mean frequency of SCE observed was 0.05, 0.16, 0.36, 0.44 and 0.52 in
control, 0.5, 1.0, 2.0 and 5.0 ppm CdCl2 respectively. It has been revealed from the results of this study that
cadmium produced genotoxic effects in fish.
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Micronucleus test, Chromosomal aberrations, Chromatid exchange, Cadmium chloride
Citation
Parveen Nuzhat, Shadab G G H A. Cytogenetic evaluation of cadmium chloride on Channa punctatus. Journal of Environmental Biology. 2012 May; 33(3): 663-666.