Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis.

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dc.contributor.authorBalne, P K
dc.contributor.authorBasu, S
dc.contributor.authorRath, S
dc.contributor.authorBarik, M R
dc.contributor.authorSharma, S
dc.date.accessioned2016-08-09T04:45:25Z
dc.date.available2016-08-09T04:45:25Z
dc.date.issued2015-10
dc.description.abstractThis study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%) and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.en_US
dc.identifier.citationBalne P K, Basu S, Rath S, Barik M R, Sharma S. Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis. Indian Journal of Medical Microbiology. 2015 Oct-Dec; 33(4): 568-571.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/176515
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=4;spage=568;epage=571;aulast=Balneen_US
dc.subjectHydroxynaphthol blueen_US
dc.subjectloop mediated isothermal amplification assayen_US
dc.subjectMPB64en_US
dc.subjectocular tuberculosisen_US
dc.subjectreal-time polymerase chain reactionen_US
dc.titleLoop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis.en_US
dc.typeArticleen_US
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