Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis

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dc.contributor.authorSingh, BKen_US
dc.contributor.authorSingh, Ken_US
dc.contributor.authorKhanna, Ren_US
dc.contributor.authorMeena, RNen_US
dc.date.accessioned2020-09-24T07:56:51Z
dc.date.available2020-09-24T07:56:51Z
dc.date.issued2020-06
dc.description.abstractBackground: It is still difficult to diagnose tuberculosis as a source of abdominal pain. Due to the lack of non-invasive diagnostic testing, the diagnosis remains a problem. Determining the role of polymerase chain reaction (PCR) in ascitic fluid in the diagnosis of abdominal tuberculosis, we can conclude whether it is sensitive/specific or not and further its use in the diagnosis of abdominal tuberculosis. Objective: The objective of the study was to determine the role of PCR in ascitic fluid in the diagnosis of abdominal tuberculosis and also to correlate the ADA levels of ascitic fluid with the PCR report. Materials and Methods: This prospective comparative study includes 41 clinically suspected abdominal tuberculosis patients (age range 15–65 years) over a period of 2 years. A detailed history, clinical evaluation, and relevant investigations, including radiology, were done in all patients. Bacteriological or histological approaches had to support the diagnosis of abdominal tuberculosis, and PCR was also tested for Mycobacterium tuberculosis in ascitic fluid. Results: The mean age of patients was 31.71±12.32 years with male:female ratio was 1.4:1. PCR was positive in 24 (58.8%) cases. A receiver operating characteristic curve showed that a cutoff value of 35.3 IU/L (AUC 0.998, P < 0.001) for the ADA level produced the best results as a diagnostic test for abdominal tuberculosis, yielding the following parameter values: Sensitivity 100%, specificity 94.1%, positive predictive value 96%, negative predictive value 100%, and diagnostic accuracy 97.56%. ADA values were significantly elevated during abdominal tuberculosis, indicating that ADA can still be a valuable diagnostic tool. Conclusion: Our findings indicate that ascitic fluid PCR is a safe tool for diagnosing it and should be tried at least before surgical intervention.en_US
dc.identifier.affiliationsDepartment of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, Indiaen_US
dc.identifier.citationSingh BK, Singh K, Khanna R, Meena RN. Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. International Journal of Medical Science and Public Health. 2020 Jun; 9(6): 368-374en_US
dc.identifier.issn2277-338X
dc.identifier.issn2320-4664
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/205650
dc.languageenen_US
dc.publisherInternational Journal of Medical Science and Public Healthen_US
dc.relation.issuenumber6en_US
dc.relation.volume9en_US
dc.source.urihttps://dx.doi.org/10.5455/ijmsph.2020.12343201930062020en_US
dc.subjectAbdominal Tuberculosisen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectAscitic Fluiden_US
dc.subjectAdenosine Deaminaseen_US
dc.titleRole of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosisen_US
dc.typeJournal Articleen_US
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