Molecular detection of drug-resistant Plasmodium falciparum mutants in Assam

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dc.contributor.authorBarman, Lipikaen_US
dc.contributor.authorSharma, Ajantaen_US
dc.contributor.authorKakati, Sanjeeben_US
dc.contributor.authorSarma, Devojit Kr.en_US
dc.contributor.authorHussain, Ezazen_US
dc.contributor.authorSaikia, Laharien_US
dc.date.accessioned2023-08-19T04:51:52Z
dc.date.available2023-08-19T04:51:52Z
dc.date.issued2023-01
dc.description.abstractBackground & objectives: The spread of drug-resistant Plasmodium falciparum ( Pf) poses a serious threat to the control and elimination of malaria. The objective of this study was to detect the molecular biomarkers of antimalarial drug resistance in Pf in patients visiting a tertiary care hospital in Assam. Methods: Malaria was first detected in fever cases using microscopy and a rapid diagnostic test (RDT), and then confirmed using PCR. Pf chloroquine resistance transporter (Pfcrt), Pf multidrug resistance-1 (Pfmdr-1), and single-nucleotide polymorphisms linked to delayed parasite clearance after treatment with artemisinin MAL 10-688956 and MAL 13-1718319 and Kelch-13 propeller (PfK-13) genes were evaluated by PCR-restriction fragment length polymorphism (RFLP). Results: Sixty nine cases of malaria were found among 300 cases of fever. Of these, 54 were positive for Pf, 47 of which were confirmed by PCR. Pfcrt-K76T mutation was seen in 96.6 per cent and Pfmdr1-N86Y mutation in 84.2 per cent of cases. Mutation was not detected in MAL10 and MAL13 genes. Sequence analysis of Kelch-13 gene showed the presence of a novel mutation at amino acid position 675. Statistically, no significant association was found between the molecular biomarkers and demographic profile, clinical presentation and outcome of the cases. Interpretation & conclusions: Molecular surveillance is essential to assess the therapeutic efficacy of the drugs against circulating Pf isolates in Assam which are found to be highly resistant to CQ. The role of the new mutation found in the Kelch-13 gene in the development of artemisinin resistance in Assam needs to be thoroughly monitored in future research.en_US
dc.identifier.affiliationsDepartment of Microbiology, Apollo Excelcare Hospitalen_US
dc.identifier.affiliationsDepartment of Microbiology, Gauhati Medical College, Guwahati, Department ofen_US
dc.identifier.affiliationsMedicineen_US
dc.identifier.affiliationsStatistics, Assam Medical Collegeen_US
dc.identifier.affiliationsRegional Medical Research Centre, Dibrugarh, Assam, Indiaen_US
dc.identifier.citationBarman Lipika, Sharma Ajanta, Kakati Sanjeeb, Sarma Devojit Kr., Hussain Ezaz, Saikia Lahari. Molecular detection of drug-resistant Plasmodium falciparum mutants in Assam. Indian Journal of Medical Research. 2023 Jul; 158(1): 55-65en_US
dc.identifier.issn0971-5916
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223572
dc.languageenen_US
dc.publisherWolters Kluwer – Medknowen_US
dc.relation.issuenumber1en_US
dc.relation.volume158en_US
dc.source.urihttps://doi.org/10.4103/ijmr.IJMR_2976_20en_US
dc.subjectArtemisininen_US
dc.subjectAssamen_US
dc.subjectchloroquineen_US
dc.subjectdrug resistanceen_US
dc.subjectmutantsen_US
dc.subjectPlasmodium falciparumen_US
dc.titleMolecular detection of drug-resistant Plasmodium falciparum mutants in Assamen_US
dc.typeJournal Articleen_US
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