Diagnostic accuracy of saliva as a specimen for detection of SARS-CoV-2 by RT-PCR

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dc.contributor.authorGupta, Men_US
dc.contributor.authorSinha, Pen_US
dc.contributor.authorGupta, Sen_US
dc.contributor.authorJain, DKen_US
dc.contributor.authorHooja, Sen_US
dc.contributor.authorVyas, N.en_US
dc.date.accessioned2023-08-10T07:32:58Z
dc.date.available2023-08-10T07:32:58Z
dc.date.issued2022-12
dc.description.abstractContext: COVID-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an emerging pandemic that is rapidly spreading with more than 114 million confirmed cases and 2.5 million deaths by far. Nasopharyngeal swab (NPS) in VTM has been used as the gold standard respiratory specimen for SARS-CoV-2 reverse-transcriptase real-time PCR (rRT-PCR) tests. But now the virus can also be detected in other clinical specimens like bronchoalveolar lavage, sputum, saliva, throat swab, blood, and stool specimens. Aims: The aim of this study was to determine the diagnostic potential of saliva as a sample in comparison to NPS for detection of SARS-CoV-2 by rRT-PCR. Settings and Design: A cross-sectional study was conducted among 256 paired samples (NPS and Saliva) received in the Department of Microbiology, SMS Medical College, Jaipur over a period of 2 months Methods and Material: NPS from individuals were collected in a sterile tube containing Viral Transport Medium™. Before swab collection, whole saliva was collected by spitting from the suspected patient into a sterile container. Both were stored at room temperature and transferred to the diagnostic laboratory within four hours of collection where extraction was done using Perkin Elmer chemagic extractor and rRT- PCR was performed using NIV, Pune mastermix. Results: Sensitivity, specificity, PPV, and NPV of RT-PCR for the diagnosis of COVID-19 in saliva were 84.26%, 100%, 100%, and 54.05%, respectively. The accuracy of detection of COVID-19 by saliva samples compared to the routinely used NPS samples (considered as the standard reference) for RT PCR was 86.72%. Conclusions: Our results show that saliva as a reliable sample type for SARS-CoV-2 detection.en_US
dc.identifier.affiliationsDepartment of Microbiology, SMS Medical College, Jaipur, Rajasthan, Indiaen_US
dc.identifier.citationGupta M, Sinha P, Gupta S, Jain DK, Hooja S, Vyas N.. Diagnostic accuracy of saliva as a specimen for detection of SARS-CoV-2 by RT-PCR. Indian Journal of Pathology & Microbiology. 2022 Dec; 65(4): 907-910en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223369
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber4en_US
dc.relation.volume65en_US
dc.source.urihttps://doi.org/10.4103/ijpm.ijpm_292_21en_US
dc.subjectNasopharyngeal swaben_US
dc.subjectrRT-PCRen_US
dc.subjectsalivaen_US
dc.subjectSARS-CoV-2en_US
dc.titleDiagnostic accuracy of saliva as a specimen for detection of SARS-CoV-2 by RT-PCRen_US
dc.typeJournal Articleen_US
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