Laboratory colonization of Aedes lineatopennis.

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dc.contributor.authorJitpakdi, Atchariyaen_US
dc.contributor.authorJunkum, Anulucken_US
dc.contributor.authorPitasawat, Benjawanen_US
dc.contributor.authorKomalamisra, Narumonen_US
dc.contributor.authorRattanachanpichai, Eumpornen_US
dc.contributor.authorChaithong, Udomen_US
dc.contributor.authorTippawangkosol, Pongsrien_US
dc.contributor.authorSukontason, Komen_US
dc.contributor.authorPuangmalee, Nateeen_US
dc.contributor.authorChoochote, Wejen_US
dc.date.accessioned2009-05-27T16:16:30Z
dc.date.available2009-05-27T16:16:30Z
dc.date.issued2005-11-28en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractAedes lineatopennis, a species member of the subgenus Neomelaniconion, could be colonized for more than 10 successive generations from 30 egg batches [totally 2,075 (34-98) eggs] of wild-caught females. The oviposited eggs needed to be incubated in a moisture chamber for at least 7 days to complete embryonation and, following immersion in 0.25-2% hay-fermented water, 61-66% of them hatched after hatching stimulation. Larvae were easily reared in 0.25-1% hay-fermented water, with suspended powder of equal weight of wheat germ, dry yeast, and oatmeal provided as food. Larval development was complete after 4-6 days. The pupal stage lasted 3-4 days when nearly all pupae reached the adult stage (87-91%). The adults had to mate artificially, and 5-day-old males proved to be the best age for induced copulation. Three to five-day-old females, which were kept in a paper cup, were fed easily on blood from an anesthetized golden hamster that was placed on the top-screen. The average number of eggs per gravid female was 63.56 +/- 22.93 (22-110). Unfed females and males, which were kept in a paper cup and fed on 5% multivitamin syrup solution, lived up to 43.17 +/- 12.63 (9-69) and 15.90 +/- 7.24 (2-39) days, respectively, in insectarium conditions of 27 +/- 2 degrees C and 70-80% relative humidity.en_US
dc.description.affiliationDepartment of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.en_US
dc.identifier.citationJitpakdi A, Junkum A, Pitasawat B, Komalamisra N, Rattanachanpichai E, Chaithong U, Tippawangkosol P, Sukontason K, Puangmalee N, Choochote W. Laboratory colonization of Aedes lineatopennis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 ; 36 Suppl 4(): 158-66en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34829
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2005_36_spp4/36sup4_158.pdfen_US
dc.subject.meshAedes --growth & developmenten_US
dc.subject.meshAnimalsen_US
dc.subject.meshAvena sativaen_US
dc.subject.meshFeeding Behavioren_US
dc.subject.meshFemaleen_US
dc.subject.meshFermentationen_US
dc.subject.meshFungal Proteinsen_US
dc.subject.meshLaboratoriesen_US
dc.subject.meshLarva --growth & developmenten_US
dc.subject.meshMaleen_US
dc.subject.meshOvipositionen_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshTriticumen_US
dc.titleLaboratory colonization of Aedes lineatopennis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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