Sand Dune Streptomyces Jb66 Native To The Great Indian Thar Desert Inhibits Multidrug-Resistant Pathogens

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dc.contributor.authorBegani, J.en_US
dc.contributor.authorLakhani, J.en_US
dc.contributor.authorHarwani, D.en_US
dc.date.accessioned2020-10-16T07:50:10Z
dc.date.available2020-10-16T07:50:10Z
dc.date.issued2019-11
dc.description.abstractThe efficacy of an actinomycete strain JB66 recovered from a sand dune soil from the Bikaner district of the Thar desert in inhibiting the growth of various bacterial pathogens was studied. The type strains Staphylococcus aureus, Shigella flexneri, Klebsiella pneumoniae and the clinical isolates Escherichia coli, multidrug-resistant S. aureus and P. vulgaris were included in the antimicrobial assays. Polyphasic characterization of JB66 isolate revealed its identity as Streptomyces (MH762010). It showed 88.99-89.24% sequence similarity with the other members of this genus and share the maximum (88.89%) similarity with Streptomyces sp. ATSC13. The strain JB66 was found to produce a high amount of extracellular L-asparaginase, catalase, gelatinase, protease, tyrosinase and urease enzymes. The partial chemical categorization of the methanolic crude extract of the JB66 strain led to the preliminary identification of various metabolic compounds. The thin-layer chromatography fractionation revealed the presence of prodigiosin pigment or chandramycin, cephalosporin or zeatin, daidzein, demethoxy rapamycin, 4,6-dihydroxy-7-methoxyisoflavone, munumbicins and amiclenomycin like compounds. Bio-autography revealed that the metabolites localized at the Rf values of 0.40, 0.46 and 0.53 in TLC profile had the actual bioactive fractions. UV-VIS spectrum absorbance maxima at 288 nm revealed the presence of an aromatic nucleus.en_US
dc.identifier.affiliationsDepartment of Microbiology, Maharaja Ganga Singh University, Bikaner-334001, Rajasthan, Indiaen_US
dc.identifier.affiliationsDepartment of Computer Science, Maharaja Ganga Singh University, Bikaner-334001, Rajasthan, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology, Maharaja Ganga Singh University, Bikaner-334001, Rajasthan, Indiaen_US
dc.identifier.citationBegani J., Lakhani J., Harwani D. . Sand Dune Streptomyces Jb66 Native To The Great Indian Thar Desert Inhibits Multidrug-Resistant Pathogens. International Journal of Pharmaceutical Sciences and Drug Research. 2019 Nov; 11(6): 289-298en_US
dc.identifier.issn0975-248X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/206263
dc.languageenen_US
dc.publisherMRI Publication Pvt. Ltd.en_US
dc.relation.issuenumber6en_US
dc.relation.volume11en_US
dc.source.urihttps://doi.org/10.25004/IJPSDR.2019.110603en_US
dc.subjectActinomycetesen_US
dc.subjectThar deserten_US
dc.subjectSand duneen_US
dc.subjectMDRen_US
dc.subjectTLCen_US
dc.subjectRapamycinen_US
dc.subjectCephalosporinen_US
dc.titleSand Dune Streptomyces Jb66 Native To The Great Indian Thar Desert Inhibits Multidrug-Resistant Pathogensen_US
dc.typeJournal Articleen_US
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