Establishment of the functional importance of thiamin carrier protein in pregnant rats by using monoclonal antibodies.

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dc.contributor.authorSubramanian, Sen_US
dc.contributor.authorKarande, A Aen_US
dc.contributor.authorAdiga, P Ren_US
dc.date.accessioned1996-04-01en_US
dc.date.accessioned2009-05-27T09:12:49Z
dc.date.available1996-04-01en_US
dc.date.available2009-05-27T09:12:49Z
dc.date.issued1996-04-01en_US
dc.description.abstractMonoclonal antibodies (mAbs) to chicken thiamin carrier protein (TCP) have been produced by hybridoma technology to identify the crucial epitopes involved in bioneutralization of the vitamin carrier. The monoclonality of these mAbs (A4C4, F3H6, H8H3, C8C1 and G7H10) was sought to be confirmed by sub-class isotyping; they all belong to IgG1, k type. The epitopes recognized by all the five mAbs are conserved in TCP from the chicken to the rat as assessed by liquid phase RIA and immunoprecipitation of 125I-labelled proteins from pregnant rat serum. Among these mAbs. passive immunization of pregnant rats with the mAb C8C1 only on three consecutive days (day 10, 11 and 12) resulted in embryonic resorption. These results demonstrate the importance of epitopic structure specified by the mAb C8C1 on TCP during pregnancy in rats.en_US
dc.description.affiliationDepartment of Biochemistry, Indian Institute of Science, Bangalore, India.en_US
dc.identifier.citationSubramanian S, Karande AA, Adiga PR. Establishment of the functional importance of thiamin carrier protein in pregnant rats by using monoclonal antibodies. Indian Journal of Biochemistry & Biophysics. 1996 Apr; 33(2): 111-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26814
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonalen_US
dc.subject.meshCarrier Proteins --immunologyen_US
dc.subject.meshEgg Proteins --immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy, Animal --immunologyen_US
dc.subject.meshRatsen_US
dc.subject.meshThiamineen_US
dc.titleEstablishment of the functional importance of thiamin carrier protein in pregnant rats by using monoclonal antibodies.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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