Antigenic heterogeneity of lipopolysaccharide among Burkholderia pseudomallei clinical isolates.

Simple item page
dc.contributor.authorAnuntagool, Nen_US
dc.contributor.authorAramsri, Pen_US
dc.contributor.authorPanichakul, Ten_US
dc.contributor.authorWuthiekanun, V Ren_US
dc.contributor.authorKinoshita, Ren_US
dc.contributor.authorWhite, N Jen_US
dc.contributor.authorSirisinha, Sen_US
dc.date.accessioned2009-05-27T16:44:36Z
dc.date.available2009-05-27T16:44:36Z
dc.date.issued2000-03-21en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractBurkholderia pseudomallei (BP) causes melioidosis, a potentially fatal human infection in the tropics. Clinical isolates from different geographical locations have similar morphological and biochemical characteristics. Although BP has been reported to possess 2 types of lipopolysaccharide (LPS) differing in the chemical structure of their O-polysaccharide (O-PS) component, earlier report demonstrated that the clinical strains exhibited identical LPS moieties. Recently, we reported antigenic similarity between the pathogenic (Ara-) and nonpathogenic (Ara+) biotypes. However, a few clinical isolates showed atypical SDS-PAGE profiles. In this study, LPS from 739 BP isolated from patients and animals in different geographical areas were extracted by proteinase K digestion method. Their SDS-PAGE profiles and their immunoreactivities with patients' sera and monoclonal antibody (MAb) to LPS were analyzed. The isolates showed 3 LPS patterns differing in the number and electrical mobility of bands in silver-stained gel. A majority of BP (711) isolates exhibited identical typical ladder pattern, 21 isolates showed atypical ladder pattern and 7 isolates did not exhibit ladder appearance. However, all LPS preparations exhibited similar endotoxic activity as determined by Limulus amebocyte lysate assay. On the other hand, there were no immunological cross reactivity between typical and atypical LPS, as judged from Western blot against homologous and heterologous sera from melioidosis patients from whom the typical and atypical LPS were isolated. Nevertheless, a Western blot profile of the typical LPS showed some variations when probed with MAb against BP LPS (9D5). Heat-killed bacteria from all LPS groups could similarly activate mouse macrophage cell line to produce nitric oxide (NO) and inducible NO synthase (iNOS).en_US
dc.description.affiliationLaboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand. narisara@tubtim.cri.or.then_US
dc.identifier.citationAnuntagool N, Aramsri P, Panichakul T, Wuthiekanun VR, Kinoshita R, White NJ, Sirisinha S. Antigenic heterogeneity of lipopolysaccharide among Burkholderia pseudomallei clinical isolates. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 ; 31 Suppl 1(): 146-52en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35909
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBurkholderia pseudomallei --isolation & purificationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshHumansen_US
dc.subject.meshLipopolysaccharides --immunologyen_US
dc.subject.meshMacrophages --metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshNitric Oxide --metabolismen_US
dc.titleAntigenic heterogeneity of lipopolysaccharide among Burkholderia pseudomallei clinical isolates.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Southeast Asian Journal of Tropical Medicine and Public Health