CD34+ hematopoietic progenitor cells enumeration and mononuclear cell count: an experience from a tertiary care centre

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dc.contributor.authorBhat, Shuaeben_US
dc.contributor.authorHussain, Saleemen_US
dc.contributor.authorNoor, Humaen_US
dc.contributor.authorShoket, Nadeemen_US
dc.date.accessioned2020-11-18T09:57:35Z
dc.date.available2020-11-18T09:57:35Z
dc.date.issued2019-02
dc.description.abstractBackground: This study was conducted to assess the CD34+ hematopoietic progenitor cells enumeration by flow cytometry and the utility of performing mononuclear cell count before performing the Stem cell enumeration. Collection was done on two consecutive days with CD34+ hematopoietic progenitor cell enumeration of both the samples. Mononuclear cell counts were done in all the patients. The purpose of the study was to do counts directly from the leukapheresis pack and see the reliability of this practice.Methods: Samples were collected from the leukapheresis pack and subjected to mononuclear cell count and CD34+ hematopoietic progenitor cells enumeration by flow cytometty before harvesting.Results: A total of 66 samples from 34 patients were taken up for the study. 76.47% of our cases were that of multiple myeloma and 17.64% of the cases were that of non Hodgkin lymphoma and 2.94% cases each of neuroblastoma and Hodgkin lymphoma. It was noted that the mononuclear cell counts correlated well with the CD34+ HPC in most of the cases with MNC being above 4 x 108 per pack per kg body weight in cases where CD34+ HPC counts were more than the desired lower limit of 2 x 106 per pack per kg body weight.Conclusions: It was observed that flow cytometric enumeration of CD34+ hematopoietic progenitor cells directly from the leukapheresis pack gave satisfactory results even without doing peripheral blood CD34+ HPCs enumeration before leukapheresis. Also, in our study we were able to set a limit of mononuclear Cell at 4 x 108 per pack/kg BW as counts beyond that always correlated with the more accurate flow cytometric method of CD34+ HPC count of more than 2 x106 per pack/kg body weight, therefore acting as a crude method for assessing the mobilization.en_US
dc.identifier.affiliationsDepartment of Pathology, Government Medical College, Anantnag, Jammu and Kashmir, Indiaen_US
dc.identifier.affiliationsDepartment of Hematopathology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Jammu & Kashmir, Indiaen_US
dc.identifier.affiliationsDepartment of Medical Oncology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Jammu & Kashmir, Indiaen_US
dc.identifier.affiliationsDepartment of Medical Oncology, Government Medical College, Jammu, Jammu & Kashmir, Indiaen_US
dc.identifier.citationBhat Shuaeb, Hussain Saleem, Noor Huma, Shoket Nadeem. CD34+ hematopoietic progenitor cells enumeration and mononuclear cell count: an experience from a tertiary care centre. International Journal of Research in Medical Sciences. 2019 Feb; 7(2): 421-427en_US
dc.identifier.issn2320-6071
dc.identifier.issn2320-6012
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/211140
dc.languageenen_US
dc.publisherMedip Academyen_US
dc.relation.issuenumber2en_US
dc.relation.volume7en_US
dc.source.urihttps://dx.doi.org/10.18203/2320-6012.ijrms20190346en_US
dc.subjectCD34+en_US
dc.subjectHematopoietic progenitor cellsen_US
dc.subjectLeukapheresisen_US
dc.subjectMononuclear cellsen_US
dc.titleCD34+ hematopoietic progenitor cells enumeration and mononuclear cell count: an experience from a tertiary care centreen_US
dc.typeJournal Articleen_US
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