Scanning electron microscopy in the assessment of sperm morphology.

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dc.contributor.authorGopalkrishnan, Ken_US
dc.contributor.authorAnand Kumar, T Cen_US
dc.date.accessioned1990-06-01en_US
dc.date.accessioned2009-05-27T06:31:29Z
dc.date.available1990-06-01en_US
dc.date.available2009-05-27T06:31:29Z
dc.date.issued1990-06-01en_US
dc.description.abstractThe advantages of using scanning electron microscope (SEM) vis-a-vis light microscope (LM) to assess sperm morphology was studied. The semen samples obtained from 15 fertile (group I) and 25 infertile (group II) men were processed by routine procedures for LM and SEM. The usually described sperm abnormalities were identifiable with greater resolution. Sperm abnormalities were significantly more in group II, as compared with group I (P less than 0.001) by both methods. Abnormalities of different regions, like thinning of midpiece, were scored higher in SEM than in LM. The abnormality of aggregation or retraction of mitochondrial sheath was clearly visible under SEM and missed by LM. Apart from detection of certain subtle abnormalities, SEM has advantages of rapid screening, accurate quantitation directly from the screen, zooming to higher magnification and automation.en_US
dc.description.affiliationInstitute for Research in Reproduction, Bombay.en_US
dc.identifier.citationGopalkrishnan K, Anand Kumar TC. Scanning electron microscopy in the assessment of sperm morphology. Indian Journal of Medical Research. 1990 Jun; 92(): 169-74en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/16659
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshHumansen_US
dc.subject.meshInfertility, Male --pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshSpermatozoa --abnormalitiesen_US
dc.titleScanning electron microscopy in the assessment of sperm morphology.en_US
dc.typeJournal Articleen_US
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