Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei.

Simple item page
dc.contributor.authorNa-ngam, Narisornen_US
dc.contributor.authorKalambaheti, Thareeraten_US
dc.contributor.authorEkpo, Pattamaen_US
dc.contributor.authorPitaksajjakul, Pannamthipen_US
dc.contributor.authorJamornthanyawat, Natsudaen_US
dc.contributor.authorChantratita, Narisaraen_US
dc.contributor.authorSirisinha, Stitayaen_US
dc.contributor.authorThamlikitkul, Visanuen_US
dc.contributor.authorChaicumpa, Wanpenen_US
dc.contributor.authorYamabhai, Montaropen_US
dc.contributor.authorRamasoota, Pongramaen_US
dc.date.accessioned2009-05-27T15:06:47Z
dc.date.available2009-05-27T15:06:47Z
dc.date.issued2008-05-21en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractRandom peptide libraries displayed by bacteriophage T7 and M13 were employed to identify mimotopes from 4 monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei. Insert DNA sequences of bound phages selected from four rounds of panning with each MAb revealed peptide sequences corresponding to B. pseudomallei K96243 hypothetical protein BPSL2046, hypothetical protein BpseP_02000035, B. pseudomallei K96243 hypothetical protein BPSS0784, B. pseudomallei 1710b hypothetical protein BURPS1710b_1104, and B. cenocepacia H12424 TonB-dependent siderophore receptor, all located at the outer membrane. The immune responses from all selected phagotopes were significantly higher than that of lipopolysaccharide. The study demonstrates the feasibility of identifying mimotopes through screening of phage-displayed random peptide libraries with B. pseudomallei MAbs.en_US
dc.description.affiliationDepartment of Veterinary Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand.en_US
dc.identifier.citationNa-ngam N, Kalambaheti T, Ekpo P, Pitaksajjakul P, Jamornthanyawat N, Chantratita N, Sirisinha S, Thamlikitkul V, Chaicumpa W, Yamabhai M, Ramasoota P. Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei. The Southeast Asian Journal of Tropical Medicine and Public Health. 2008 May; 39(3): 443-51en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/31945
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2008_39_3/10-4213.pdfen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial --immunologyen_US
dc.subject.meshAntibodies, Monoclonal --geneticsen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshAntigens, Bacterial --immunologyen_US
dc.subject.meshBacterial Vaccines --immunologyen_US
dc.subject.meshBacteriophage M13 --geneticsen_US
dc.subject.meshBacteriophage T3 --geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBurkholderia pseudomallei --immunologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshEpitopes --geneticsen_US
dc.subject.meshMelioidosis --immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPeptide Libraryen_US
dc.subject.meshPeptides --geneticsen_US
dc.titleImmune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
tsajtmph_2008v39n3p443.pdf
Size:
78.87 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Southeast Asian Journal of Tropical Medicine and Public Health