Detection of Vibrio Cholerae From Patient, Coarrier, Water and Other Environment Samples in Areas of Phop Phra District, Tak.
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Date
2011-01-26
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Bulletin of the Department of Medical Sciences - วารสารกรมวิทยาศาสตร์การแพทย์
Abstract
In Thailand there are occasional outbreaks of cholera near Thai-Myanmar Border, PropPhra district. It is a diarrhoeal disease caused by a gram negative bacteriaVibrio cholerae, which is anautochthonous inhabitant of fresh and marine waters worldwide systems. Only O1 and O139 serogroupsofV. cholerae have potential to cause of dehydrating diarrhoeal condition called cholera, that can producecholera toxin controlled byctxAB gene. In this study we detected Vibrio cholerae from rectal swab of patients,carriers and normal hosts as well as collected water and environment samples for a total 1,452 samples.We performed conventional culture method, and using duplex PCR method targetedV.choleraespecies-specific gene (ompW) and cholera toxin encoding gene (ctxA) as a screening test on anyyellow bacterial colony on TCBS.Vibrio cholerae O1, El Tor, Inaba could be found 0.48 % (5 rectalswabs and 2 environment samples) andVibrio cholerae nonO1/nonO139 8.82 % (6 rectal swabs and122 environment samples). All of these strains were resistant to colistin 71.9 %, ampicillin 28.9 % andtrimethoprim/ sulfamethoxazole 11.1%. The results of duplex-PCR method were identical to thosebiochemical and specific antiserum testing method. It can be used as a rapid method for detection andidentification ofV. cholerae. This experiment can be a useful for prevention and control of cholera outbreak.Key word : detection, duplex PCR, Vibrio cholerae
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Bulletin of the Department of Medical Sciences - วารสารกรมวิทยาศาสตร์การแพทย์; Vol. 51 No. 3-4 July - December 2009; 187-203