Ocular conjunctival microbiome profiling in dry eye disease: A case control pilot study

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dc.contributor.authorGupta, Noopuren_US
dc.contributor.authorChhibber-Goel, Jyotien_US
dc.contributor.authorGupta, Yogitaen_US
dc.contributor.authorMukherjee, Souviken_US
dc.contributor.authorMaitra, Arindamen_US
dc.contributor.authorSharma, Amiten_US
dc.contributor.authorTandon, Radhikaen_US
dc.date.accessioned2023-08-25T06:36:44Z
dc.date.available2023-08-25T06:36:44Z
dc.date.issued2023-04
dc.description.abstractPurpose: Keratoconjunctivitis sicca (KCS) or dry eye disease (DED) is a multifactorial disease that results in discomfort, visual disturbance, and tear film instability with potential damage to the ocular surface. A pilot study was undertaken to determine if there were any major substantial differences in the ocular microbiome in DED patients versus healthy controls. Methods: The bacterial communities residing in the conjunctiva of patients with DED (n = 4) and healthy controls (n = 4) were assessed by 16S ribosomal RNA (rRNA) gene sequencing of the V4–V5 region. Results: The phyla Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes were most dominant and accounted for 97% and 94.5% of all bacterial sequences in patients and controls, respectively. At the genus level, 27 bacterial genera were found with more than two?fold difference between patients and controls. Four of these – Acinetobacter, Corynebacterium, Lactobacillus, and Pseudomonas spp. – dominated the ocular microbiome of all subjects, but were proportionately lower in DED (16.5%) compared to controls (37.7%). Several bacterial genera were found to be unique in DED (34) and controls (24). Conclusion: This pilot study is an attempt to profile the ocular microbiome in patients with DED that demonstrated a higher concentration of microbial DNA compared to controls, with Firmicutes phyla dominating the bacterial population in patients with DED.en_US
dc.identifier.affiliationsDr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsMolecular Medicine, International Centre for Genetic Engineering and Biotechnology, New Delhi, Indiaen_US
dc.identifier.affiliationsNational Institute of Biomedical Genomics, Kalyani, West Bengal, Indiaen_US
dc.identifier.affiliationsICMR-National Institute of Malaria Research, New Delhi, Indiaen_US
dc.identifier.citationGupta Noopur, Chhibber-Goel Jyoti, Gupta Yogita, Mukherjee Souvik, Maitra Arindam, Sharma Amit, Tandon Radhika. Ocular conjunctival microbiome profiling in dry eye disease: A case control pilot study. Indian Journal of Ophthalmology. 2023 Apr; 71(4): 1574-1581en_US
dc.identifier.issn1998-3689
dc.identifier.issn0301-4738
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/224970
dc.languageenen_US
dc.publisherAll India Ophthalmological Societyen_US
dc.relation.issuenumber4en_US
dc.relation.volume71en_US
dc.source.urihttps://doi.org/10.4103/ijo.IJO_1756_22en_US
dc.subject16S rRNAen_US
dc.subjectconjunctivaen_US
dc.subjectdry eye diseaseen_US
dc.subjectocular microbiomeen_US
dc.subjectocular surfaceen_US
dc.titleOcular conjunctival microbiome profiling in dry eye disease: A case control pilot studyen_US
dc.typeJournal Articleen_US
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