Expression of HER2/neu in gastric adenocarcinoma and its correlation with serum HER2/neu level and E-cadherin expression

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dc.contributor.authorPandey, Ien_US
dc.contributor.authorMisra, Ven_US
dc.contributor.authorPandey, ATen_US
dc.contributor.authorVerma, A.en_US
dc.date.accessioned2023-08-10T07:32:18Z
dc.date.available2023-08-10T07:32:18Z
dc.date.issued2022-03
dc.description.abstractAim: To assess HER2/neu expressions and correlate with E-cadherin and Serum HER2 level in gastric carcinoma. Method: 31 gastric biopsies and 1 resected specimen were taken in the study with patient details and stained with H and E for histopathological details following Lauren's classification. Immunohistochemistry for HER2 and E-cadherin expression was conducted followed by serum HER2/neu ELISA. Result: Adenocarcinoma with 61% diffuse, 29% intestinal, and 10% other type were observed with predominant HER2 immunoexpression in intestinal-type than in diffuse-type adenocarcinoma. Other observations marked 44% as 3+/positive and 56% as 2+/equivocal in intestinal type while 26% cases as 3+/positive, 69% as 2+/equivocal, and 1% as 1+/negative were observed in diffuse type. The data presented 33% membranous positivity and 67% both membranous + cytoplasmic positivity in intestinal type while 2% showed membranous positivity, 47% both membranous + cytoplasmic, and 42% only cytoplasmic positivity in diffused type. On comparing the localization pattern of HER2 and E-cadherin, 25% of cases showed membranous staining while 50% of cases showed membranous with cytoplasmic staining for both. No cytoplasmic HER2 staining as well as no any staining for E-cadherin was shown by 6% cases. Conclusion: Thus, it can be concluded that cytoplasmic expression of HER2 in gastric adenocarcinoma (mainly diffuse type) may be due to shedding of its extracellular domain, leading to loss of membranous E-cadherin expression on immunohistochemistry. The loss of membranous expression of E-cadherin and increased serum HER2 ELISA were correlated well with these findings.en_US
dc.identifier.affiliationsDepartment of Pathology, MotiLal Nehru Medical College, Prayagraj, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsCentre of Biotechnology, University of Allahabad, Prayagraj, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsBioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, SHUATS, Prayagraj, Uttar Pradesh, Indiaen_US
dc.identifier.citationPandey I, Misra V, Pandey AT, Verma A.. Expression of HER2/neu in gastric adenocarcinoma and its correlation with serum HER2/neu level and E-cadherin expression. Indian Journal of Pathology & Microbiology. 2022 Mar; 65(1): 35-41en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223236
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber1en_US
dc.relation.volume65en_US
dc.source.urihttps://doi.org/10.4103/ijpm.ijpm_133_21en_US
dc.subjectE-cadherinen_US
dc.subjectgastric adenocarcinomaen_US
dc.subjectHER2/neuen_US
dc.subjectimmunohistochemistryen_US
dc.subjectserum ELISAen_US
dc.titleExpression of HER2/neu in gastric adenocarcinoma and its correlation with serum HER2/neu level and E-cadherin expressionen_US
dc.typeJournal Articleen_US
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