Effect of rifampicin & isoniazid on cytochrome P-450 in mycobacteria.

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dc.contributor.authorRamachandran, Geethaen_US
dc.contributor.authorGurumurthy, Premaen_US
dc.date.accessioned2002-10-05en_US
dc.date.accessioned2009-05-27T06:36:46Z
dc.date.available2002-10-05en_US
dc.date.available2009-05-27T06:36:46Z
dc.date.issued2002-10-05en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Rifampicin and isoniazid are the most important first line drugs used in the treatment of tuberculosis. These drugs are also used in combination with other medications to treat co-infections. It, therefore, becomes important to study the effect of these drugs on the drug metabolizing system, namely, cytochrome P-450, not only in the host but also in the bacteria. We report the effect of rifampicin and isoniazid on the cytochrome P-450 activity in Mycobacterium smegmatis and M. tuberculosis H37Rv. METHODS: Subinhibitory concentrations of rifampicin and isoniazid were added to the organisms after they had attained the growth phase and cytochrome P-450 activity was estimated in the membranous fractions of the bacteria at different time points. RESULTS: Rifampicin was able to significantly enhance cytochrome P-450 in both M. smegmatis and M. tuberculosis H37Rv. Isoniazid was found to inhibit cytochrome P-450 in M. tuberculosis H37Rv, while there seemed to be no effect in M. smegmatis. INTERPRETATION & CONCLUSION: We report here the effect of rifampicin and isoniazid on mycobacterial cytochrome P-450. These findings are similar to those found in eukaryotic organisms. The role of mycobacterial cytochrome P-450 in the metabolism of drugs within the bacteria needs to be elucidated.en_US
dc.description.affiliationTuberculosis Research Centre (ICMR), Chennai, India.en_US
dc.identifier.citationRamachandran G, Gurumurthy P. Effect of rifampicin & isoniazid on cytochrome P-450 in mycobacteria. Indian Journal of Medical Research. 2002 Oct; 116(): 140-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/17091
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAntitubercular Agents --pharmacologyen_US
dc.subject.meshCytochrome P-450 Enzyme System --drug effectsen_US
dc.subject.meshIsoniazid --pharmacologyen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshMycobacterium --enzymologyen_US
dc.subject.meshRifampin --pharmacologyen_US
dc.titleEffect of rifampicin & isoniazid on cytochrome P-450 in mycobacteria.en_US
dc.typeJournal Articleen_US
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