Method Development of Benzo(a)Pyrene in Charcoal–Grilled Animal Tissue
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Date
2011-01-26
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Bulletin of the Department of Medical Sciences - วารสารกรมวิทยาศาสตร์การแพทย์
Abstract
Benzo(a)pyrene (BaP) is one of the compound of polycyclic aromatic hydrocarbons incharcoal–grilled animal tissue as a result of incomplete combustion or pyrolysis of organic matter which areprobable human carcinogen. Determination of benzo(a)pyrene was studied and developed. The sample wassaponififfiified with base and separated by solid phase extraction. Sep-Pak C18 was used for clean-up procedure.BaP was detected and quantitatively determined by HPLC-Fluorescence detector. The method was validatedby using grilled pork as represented matrix for charcoal–grilled animal tissue. The results showed thatthe limit of detection and limit of quantitation were 0.3μg/kg and 0.5 μg/kg, repectively. The linearworking range was 0.5 – 10.0μg/kg with the correlation coeffiffiificient of 0.9893. Accuracy of the methodwas shown by % recovery of spiking BaP standard to sample matrix throughout the linear range was 88 – 95.Precision was shown by % relative standard deviation was 4.8 – 15.4. From validation data, the method’sperformance characteristics of the developed method were complied to Commission Directive 2005/10/EC(4 February 2005). On the conclusion, the developed method was valid and fiffiifit for using as analytical methodfor monitoring hazard of intake of BaP from charcoal grilled food.Key words : Benzo(a)pyrene, Food, Method validation, Polycyclic aromatic hydrocarbons
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Bulletin of the Department of Medical Sciences - วารสารกรมวิทยาศาสตร์การแพทย์; Vol. 51 No. 3-4 July - December 2009; 177-186