Browsing by Author "Zhou, Lin"
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Item Fluvastatin, a lipophilic statin, induces apoptosis in human hepatocellular carcinoma cells through mitochondria-operated pathway.(2010-12) Zhang, Wu; Wu, Jian; Zhou, Lin; Xie, Hai-Yang; Zheng, Shu-SenFluvastatin, a lipophilic statin, was known to inhibit proliferation and induce apoptosis in many cancer cells. Its potential anticancer was evaluated in three hepatocellular carcinoma (HCC) cell lines (HepG2, SMMC-7721 and MHCC-97H). Cells were treated with fluvastatin in vitro and its effect on cell proliferation, cell cycle, invasion and apoptosis was determined. Mechanism of apoptosis induced by fluvastatin on HCC cell lines was also investigated through western blotting and mitochondrial membrane potential (MMP) analysis. It was observed that fluvastatin inhibited proliferation of HCC cells by inducing apoptosis and G2/M phase arrest in a dose-dependent manner. The results of cell invasion assay revealed that fluvastatin significantly decreased the invasion potency of HCC cells. A mitochondria-operated mechanism for fluvastatin induced apoptosis might be involved and was supported by Western blotting and MMP analysis. After fluvastatin treatment, expression of Bcl-2 and procaspase-9 were downregulated, cytochrome c (cytosolic extract), Bax and cleaved-caspase-3 protein expression were increased. Furthermore, a breakdown of MMP in HCC cells was observed. To conclude, these results have provided a rationale for clinical investigations of fluvastatin in future as a potential anticancer reagent for growth control of HCC.Item Individualized and inverse optimized needle configuration for combined intracavitary-interstitial brachytherapy in locally advanced cervical cancer(Wolters Kluwer India Pvt. Ltd., 2020-01) Zhang, Daguang; Yang, Zhiyong; Jiang, Shan; Zhou, Lin; Zhou, Zeyang; Wang, WeiObjectives: The aim of this study is to address the limitation of combined intracavitary-interstitial (IC/IS) brachytherapy (BT) in locally advanced cervical cancer using standardized applicators and to determine the optimal dose distribution in patients with challenging tumors, innovative methods of customizing and optimizing the IS needle configuration for combined IC/IS BT are proposed and investigated. Materials and Methods: A software module that could customize the IS needle configuration and subsequently generate the digital model of guiding template for three-dimensional printing was developed and integrated into our in-house treatment planning system for BT. The inverse optimization method based on the technique of mixed-integer linear programming was introduced to determine the needle tracks out of a candidate pool and dwell times at corresponding locations to best meet dose objectives. A treatment planning study was conducted to evaluate the feasibility and performance of the proposed methods. Results: The workflow for combined IC/IS BT with customized and inverse optimized IS needle configuration was presented. Dosimetric results of the treatment planning study showed that sufficient target coverage could be obtained with the customized IS needle configuration for challenging cases. The proposed dose-based optimization method for IS needle configuration was feasible and effective. Improved target coverage and organ-at-risk sparing were achieved using the inverse planning method. Conclusions: Using the proposed methods of customizing and optimizing the IS needle configuration, the limitation in the standardized design of combined IC/IS applicators can be addressed, and sufficient target coverage is obtained in cervical cancer patients with unfavorable tumor topography and/or extra lateral expansion.Item Integrating Clinical Practice and Research Skills into Comprehensive Capability for Translational Medicine, Zhejiang Experience on the Hepato-bilio-pancreatic Surgeon Program.(South East Asia Regional Association for Medical Education., 2014-06) Chen, Xinhua; Jiang, Guoping; Yin, Shengyong; Xie, Haiyang; Zhou, Lin; Zheng, ShuseA highly skilled surgical team relies on a standardized training program. This article presents a comprehensive description on the current status, problems, and strategies in Hepato-bilio-pancreatic surgery education reform in Zhejiang University School of Medicine (ZJU), China. A multiple-discipline joint training program was set up by integrating the basic laboratory and clinical research as well as routine clinical practice. Reform includes resident training in the Royal College of Surgeons of Edinburgh, curriculum resetting, surgery textbook rewriting, clinical teaching and training bases construction, teaching methods improvement, English reading, writing and reporting skills training and a research assignment. ZJU establishes rigorous processes for managing teaching quality and ensuring graduates promote evidence-based surgical practice. ZJU has built its leading position in the Hepato-Bilio-Pancreatic surgery in China. In 2011, 6 post-doctoral researchers, 52 Ph.D. students and 83 master students were enrolled in the 7year MD program and 8year Ph.D. MD/Ph.D. program. The admission scores have been listed as the top three of the 264 master degrees programs and 181 doctoral degrees programs in Zhejiang University, which ranks among the top three in all Chinese Universities. Historical experience and the advanced experience of Western countries are adopted as a reference to build up a surgical education system with Chinese characteristics to achieve a continuous, sound, and rapid development of surgical education in ZJU, China. However, there are differences relating to the healthcare systems of China and Western countries. These differences present major challenges for the internationalization of surgical education. This article may facilitate the process of surgical training development in a global context.