Browsing by Author "Zhang, Jie"
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Item Analysis of water quality factors influencing the speciation of inorganic nitrogen using GRA.(2011-09) Zhang, Jie; Li, Da-Ping; Gao, Ping; Tao, Yong; Wang, Xiao-Mei; He, Xiao-HongBased on the water quality data from 2006 to 2008, grey relational analysis (GRA) is used to analyze factors that may have influence on the speciation of inorganic nitrogen in the Chengdu section of middle Min river. The results show that water temperature, changing from 20.2±2.7, 13.4±5.7 and 16.8±5.6oC, is the first restrictive factor for the speciation of inorganic nitrogen; it is negatively correlated with the ratio of total ammonia nitrogen to total inorganic nitrogen contents [m(AN)/m(TIN)] in three different periods of wet season, dry season and year-round. The average pH values for years, in wet and dry periods are 7.6±0.4, 7.3±0.3 and 7.8±0.2, respectively, and have different influences in different seasons. It is the second restrictive factor and positive correlation between pH and m(AN)/m(TIN) in wet season and through the year yet it is the fourth factor in dry seasons. The values of dissolved oxygen (DO), which are 4.6±1.4, 4.6±2.4, 4.6±2.0 respectively, is the third factor and negatively correlates with m(AN)/m(TIN) in third different periods. The chemical oxygen demand (COD) indirectly inhibits the nitrifying bacteria because the DO is depleted in the decomposition of organic matter by heterotrophic bacteria, showing the positive correlation. As the alkalinity can meet the requirement of nitrification in wet season and through the year, it is not restrictive factor. However, it is the second restrictive factor in dry season because of low content of alkalinity inhibiting the growth of nitrifying bacteria.Item Application value of monitoring of percutaneous partial pressure of carbon dioxide/oxygen for patients in ICU after general anesthesia(CSIR-NIScPR, 2022-07) Zhang, Juan; Luo, Huilin; Yang, Yunyun; Zhang, JieFor patients in ICU under mechanical ventilation, monitoring of percutaneous partial pressure of CO2 and O2 after general anesthesia is crucial as excessive or insufficient ventilation may prove to be fatal. However, the correlation between percutaneous monitoring and arterial blood gas analysis still remains unclear. Here, we studied the application value of monitoring of PtcCO2/PtcO2 for patients in ICU after general anesthesia for better management of patients in ICU on ventilation. A total of 195 eligible patients were selected. After transfer, when PtcCO2 and PtcO2 were stable (10-15 min), they were recorded as transfer-in values. Partial pressure of carbon dioxide (PaCO2), partial pressure of oxygen (PaO2), lactate, PtcO2 and PtcCO2 were recorded. Vital signs and hemodynamics data were also recorded. There were significant positive correlations between PtcCO2 and PtcO2 (r = 0.876), and between PtcO2 and PaCO2 (r = 0.817) (P <0.01). PaO2 was higher than PtcO2 (P <0.01). There were significant positive correlations between PtcCO2/PtcO2 and PaCO2/PaO2 at different time points (T0: upon transfer-in; T1: before extubation; T2: upon transfer-out, P <0.01). At different oxygen inhalation concentrations (21, 40 and 50%), PtcCO2 and PtcO2 were all well linearly correlated. The overall PtcO2/PaO2 was (0.75±0.12). When FIO2 values were 21, 40 and 50%, PtcO2/PaO2 values were similar (P >0.05). PtcCO2 and PtcO2 or PtcO2 and PaCO2 are significantly positively correlated, without significant differences between different time periods or oxygen concentrations. PtcCO2 and PtcO2 can predict the changes of PtcO2 and PaCO2, which is of great significance to the early observation of oxygenation changes, adjustment of ventilator parameters and improvement of prognosis. PtcCO2/PaO2 values are similar at different oxygen concentrations, as an eligible index for the postoperative evaluation of tissue perfusion status and hemodynamic level.Item DNA extraction from crayfish exoskeleton.(2011-12) Li, Yanhe; Wang, Weimin; Liu, Xiaolian; Luo, Wei; Zhang, Jie; Gul, YasmeenCrayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.Item Effects of poly (ADP-ribosyl) polymerase (PARP) inhibitor on cisplatin resistance & proliferation of the ovarian cancer C13* cells.(2013-03) Zhang, Jingjing; Kan, Yanyan; Tian, Yongjie; Wang, Zhe; Zhang, JieBackground & objectives: Drug resistance is the primary cause of failure in the treatment of cancers. It has been suggested that the enhancement of DNA repair capability may be responsible for the drug resistance of the tumour cells, and poly(ADP-ribosyl)ation plays an important role in DNA repair. This study investigated the effect of PARP inhibitor 3-aminobenzamide (3-AB) on the cisplatin resistance and proliferation of the cisplatin-resistant ovarian cancer C13* cells in vitro. Methods: C13* cells were treated with various concentrations of 3-AB in vitro. MTT assay was used to determine the effect of 3-AB on the cisplatin sensitivity and proliferation of cells. The expression levels of PARP-1 mRNA and protein in the C13* cells were examined using reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, and changes caused by 3-AB treatment were investigated. Immunofluorescence microscopy was used to detect the localization and expression of the PARP-1 proteins before and after treatment with 5 mmol/l 3-AB. Results: The inhibitory ratio and the cisplatin sensitivity of C13* cells significantly increased with the increase of the concentration of 3-AB (P<0.05). The RT-PCR analysis revealed that the expression of PARP-1 mRNA was decreased when platinum (Pt) and 3-AB were combined. The expression levels of PARP-1 protein were decreased by 23.15 ± 2.53, 59.11 ± 2.23 and 73.24 ± 3.88 per cent, respectively, in C13* cells with the increase of the concentration of 3-AB (P<0.05). The immunofluorescence microscopy results indicated that the expression level of PARP-1 protein was significantly decreased after treatment with 3-AB (P<0.05). Interpretation & conclusions: 3-AB inhibited the proliferation activity of C13* cells, and increased the cellular sensitivity to cisplatin. Our findings show that the PARP inhibitor 3-AB can downregulate the expression of PARP-1 at transcriptional and translational levels in C13* cells.Item Prevalence of MET exon 14 skipping mutation in pulmonary sarcomatoid carcinoma patients without common targetable mutations: A single-institute study(Wolters Kluwer India Pvt. Ltd., 2019-08) Yu, Yongfeng; Zhang, Qing; Zhang, Jie; Lu, ShunBackground: Pulmonary sarcomatoid carcinoma (PSC) is a rare histologic subtype of nonsmall cell lung cancer with limited targeted treatment options. In this study, we aimed to investigate the prevalence of MET ex14 skipping mutation in PSC patients without common targetable mutations in EGFR, KRAS, ALK, ROS1, and RET. Materials and Methods: In total, 46 resected specimens of PSC without these mutations were assessed for MET ex14 skipping mutation by next-generation sequencing (NGS) based on the Oncomine Focus Assay libraries. Results: Among 52 cancer-relevant genes included in the targeted NGS panel, the MET ex14 skipping mutation was the only mutation identified in our cohort, which was present in 4 (9%) of 46 patients. For patients with METex14 skipping mutation, the median overall survival (OS) was 35 months (1050 days) compared with a median OS of 27 months (807 days) for those without METex14 skipping mutation (hazard ratio [HR] = 0.59, P = 0.488). The median disease-free survival (DFS) in METex14 skipping mutation-positive patients was 18 months (540 days) compared with a median DFS of 13.6 months (408 days) for negative patients (HR = 0.76, P = 0.680). Conclusions: These findings reflect the prevalence of MET ex14 skipping mutation as up to 9% in Chinese patients with PSC negative for other common targetable mutations, allowing provision of appropriate genetic counseling and treatment in these patients. A larger population-based study is warranted to determine the clinicopathological and prognostic implications of MET ex14 skipping mutation in PSC