Browsing by Author "Tangthongchaiwiriya, Kuntida"

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    Analysis of salivary gland proteins of the mosquito Armigeres subalbatus.
    (2005-01-24) Siriyasatien, Padet; Tangthongchaiwiriya, Kuntida; Jariyapan, Narisara; Kaewsaitiam, Sakchai; Poovorawan, Yong; Thavara, Usavadee
    Quantitative studies of total salivary gland protein of Armigeres subalbatus mosquito revealed that the total salivary gland protein increased dramatically during the five days after emergence as adults. The amount of salivary gland protein of female and male mosquitos at day five after adult emergence were on the average 11.55 and 1.32 microg/pair gland respectively. SDS-PAGE studies showed that salivary gland protein profiles of Armigeres subalbatus demonstrated 9 major polypeptide bands of 68, 65, 60, 55, 40, 30, 28, 21, and 15 kDa. The 21 and 65 kDa bands were found only in the distal lateral region of the mosquito salivary gland and were depleted after the female mosquito took a blood meal.
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    Decrease of mosquito salivary gland proteins after a blood meal: an implication for pathogenesis of mosquito bite allergy.
    (2005-09-21) Siriyasatien, Padet; Tangthongchaiwiriya, Kuntida; Kraivichian, Kanyarat; Nuchprayoon, Surang; Tawatsin, Apiwat; Thavara, Usavadee
    Salivary gland protein profiles ofAedes aegypti (L.) and Culex quinquefasciatus (Say) pre- and post-blood feeding were analyzed. SDS-PAGE studies before blood feeding of Ae. aegypti demonstrated 8 major polypeptide bands of 20, 35, 37, 42, 45, 47, 70 kDa and a high molecular weight band >118 kDa, whereas those of Cx. quinquefasciatus demonstrated 9 major polypeptide bands of 20, 26, 36, 38, 45, 47, 49 kDa and 2 high molecular weight bands >118 kDa. After a blood feeding, salivary gland polypeptides of Ae. aegypti at 35, 37, 45, 47, 70 kDa and high molecular weight band >118 kDa were depleted, while the polypeptide bands of 20, 26, 36, 38 kDa were depleted in Cx. quinquefasciatus. The presented study suggests that these major polypeptides were introduced into vertebrate hosts when a mosquito took a blood meal. Further investigation in molecular, biochemical and immunological aspects of these salivary gland polypeptides may provide information for better understanding in the role of these proteins in mosquito bite allergy.