Browsing by Author "Swaminathan, K"
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Item Genetic diversity among toxigenic clostridia isolated from soil, water, meat and associated polluted sites in South India.(2009-10) Sathish, S; Swaminathan, KPurpose: To investigate the genetic diversity of toxigenic Clostridium strains isolated from soil, water, meat and its associated polluted sites of Southern India. Materials and Methods: A total of 27 identified isolates of six different toxigenic clostridial species including C. bifermentans , C. botulinum , C. chauvoei , C. ramosum , C. tetani and C. novyi were isolated and characterized by conventional DNA restriction digestion analysis (REA) and by whole-cell and excretory protein patterns on SDS-PAGE. Results: The DNA fragment size ranged from 35-160 kilobases and the protein bands 30-200 KDa, followed by numerical analyses and phylogenetic analyses. Whole-cell protein banding pattern were unique with strains of C. chauvoei , C. novyi and C. ramosum . All the strains were heterogeneous and distinct in restriction digestion pattern and excretory protein patterns. Conclusion: These analyses contribute to the understanding of prevalence of toxigenic clostridial species and phylogeny within the species and assist in development of improved diagnostics and therapeutics for the treatment of clostridial infections.Item Growth and amylase production by Aspergillus oryzae during solid state fermentation using banana waste as substrate.(2005-10-08) Ragunathan, R; Swaminathan, KAspergillus oryzae, isolated from the starch rich litter soil, produced amylase when banana fruit stalk was used as substrate in a solid state fermentation system. The effects of incubation period, pH, temperature and various carbon sources on the production of amylase were studied. A maximum yield of 380U/mg was recorded on 96th hour of incubation. The amylase is tolerant to wide range of initial culture pH values (3 to 8) and temperature (25 to 35 degrees C), with an optimum pH of 5 and temperature of 35 degrees C. In SSF addition of starch (1%) increased the amylase production, when compared with other carbon sources used.Item Incidence of Campylobacter in diarrhoeal cases and in control population.(1986-10-01) Narayan, K; Shah, S C; Pole, S L; Dalal, P J; Kelkar, S S; Bhatkhande, R; Dalal, N J; Swaminathan, K; Talwalkar, V C