Browsing by Author "Surathin, Kamhaeng"
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Item Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand.(2002-03-16) Choochote, Wej; Rongsriyam, Yupha; Leemingsawat, Somjai; Jitpakdi, Atchariya; Komalamisra, Narumon; Surathin, Kamhaeng; Somboon, Pradya; Chen, Bin; Wongkamchai, Sirijit; Jariyapan, Narissara; Tippawangkosol, Pongsri; Pitasawat, Benjawan; Riyong, DoungratHybridization tests of laboratory-raised, isolines of Anopheles minimus, species A and C were conducted by induced copulation. The three isolines were established based on three morphological variants of wild-caught, fully engorged females and two distinct types of metaphase chromosomes. They were An. minimus species A: V form (X1,Y1), M form (X2,Y1); species C: P form (X3,Y2). The results of reciprocal and back crosses indicated that the two morphologically variant forms of species A were genetically compatible, providing viable progeny and completely synaptic salivary gland polytene chromosomes, whereas they were genetically incompatible with species C and/or the P form. Hybrid progeny was only obtained from both forms of species A females x species C males, but asynaptic salivary gland polytene chromosomes on 3L and partial development of ovarian follicles in females were seen. Back crosses of F1 hybrid males with parental species A females provided viable progeny, while back crosses of F1 hybrid females with parental species C males provided progeny of low viability and adult males with abnormal spermatozoa, suggesting the partial reproductive isolation of An. minimus species A and C.