Browsing by Author "Srivastava, S K"
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Item Accumulation of copper and inhibition of lactate dehydrogenase activity in human senile cataractous lens.(1969-01-01) Nath, R; Srivastava, S K; Singh, KItem Age-dependent induction of soluble creatine phosphokinase of the cardiac muscle of rats by hydrocortisone & cortisone.(1979-12-01) Srivastava, S K; Kanungo, M SItem Ailing healthcare in India.(2006-11-09) Srivastava, S KItem Changes in polyamine levels on infection of plants by Cuscuta reflexa.(1976-09-01) Naik, B I; Mehta, K K; Srivastava, S KItem Characterization of trypsin extractable antigens of Leptospira interrogans serovars pomona, bataviae & L. biflexa serovar patoc.(1989-07-01) Premlatha, M M; Srivastava, S KLeptospira strains after treatment with trypsin released an antigen (Tx) which consisted of protein (4.2 mg/ml), carbohydrate (0.39 mg/ml) and hexosamine (0.025 mg/ml). Immunodiffusion, immunoelectrophoresis and indirect haemagglutination tests revealed serological cross reactions among the three strains used. Inhibition of microscopic agglutination reaction by the Tx antigen was observed in homologous system only. The antigen was partially sensitive to heat (80 degrees C for 10 min) as detected by immunodiffusion and microscopic agglutination inhibition. These data revealed the presence of serovar-specific and genus-specific antigens on Leptospira strains. It is suggested that this antigen could be useful in developing a serodiagnostic test for leptospirosis.Item A clinical study of measles.(1973-02-01) Nigam, P; Tandon, V K; Sahai, I; Srivastava, S KItem Cloning and sequencing of 28 kDa outer membrane protein gene of Brucella melitensis Rev. 1.(2005-09-29) Chaudhuri, Pallab; Kumar, S Vinoth; Prasad, Rajeev; Srivastava, S K; Yadav, M PBrucella melitensis is an organism of paramount zoonotic importance. The 28 kDa outer membrane protein (OMP) is one of the immunodominant antigens of B. melitensis. The gene encoding 28 kDa OMP (omp28) has been amplified from B. melitensis Rev. 1 strain. A PCR product of 753 bp, encoding complete omp28 gene of B. melitensis, was obtained. The gene was further cloned and sequenced. The nucleotide sequence of B. melitensis Rev. 1 strain showed substitution of 2 nucleotides from that of 16M strain.Item Comparative analysis of protein profiles of wild virulent (E156) and aroA-htrA double deletion mutant vaccine strain (S30) of Salmonella enterica subsp. enterica serovar Abortusequi under in vivo and in vitro growth conditions.(2008-09-28) Chandra, Mudit; Singh, B R; Srivastava, S K; Chaudhry, P; Agrawal, Ravi Kant; Sharma, AnupmaIn the present study, cell lysate and cell supernatant of the both strains i.e., virulent wild type (E156) and mutant (S30) vaccine strains of Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi), grown under varied in vivo and in vitro conditions were subjected to SDS PAGE and western blotting (using rabbit hyperimmune serum). Variation in growth conditions did not have any significant effect on expression of different proteins. SDS PAGE of E156 and S30 cell lysate (CL) revealed 26 and 28 bands, respectively with 3 prominent proteins of 71, 46 and 42 kDa in cell lysate of E 156 and 4 prominent proteins 71, 65, 46 and 40 kDa in S30 strain. The cell supernatant (CS) from both the strains, subjected to SDS PAGE, exhibited similarity in protein profile among these strains, however three bands of 65, 53 and 40 kDa were more prominent in CS preparation of S30, whereas a 56 kDa protein was prominent in CS of E156. Western blotting of E156 and S30 revealed 3 unique proteins of 65, 53 and 40 kDa present in CS preparation of S30 strains which could be used for differentiation of mutant and wild strains and also in development of test for differentiating vaccinated animals from naturally infected.Item Comparative studies on arginase of the liver of some vertebrates.(1976-01-01) Patnaik, S K; Paulose, C S; Srivastava, S K; Misra, M; Singh, A K; Kumar, DItem Comparative studies on myosin ATPase of skeletal muscle of mammals of different habitats.(1977-09-01) Srivastava, S K; Das, R; Manjula,; Yadava, R N; Kanungo, M SItem Congenital cleft hand and cleft foot.(1991-05-01) Patond, K R; Kumar, N; Srivastava, S KItem Cost variation of some commonly used antimicrobial agents.(1997-07-01) Srivastava, S K; Desai, S VItem Detection of Pasteurella multocida in experimentally infected embryonated chicken eggs by PCR assay.(2006-04-25) Shivachandra, S B; Kumar, A A; Gautam, R; Joseph, S; Chaudhuri, P; Saxena, M K; Srivastava, S K; Singh, NemApplicability of polymerase chain reaction (PCR) assay to detect Pasteurella multocida in experimentally infected embryonated chicken egg was assessed in the present study. PCR assay rapidly and specifically detected the genome of P. multocida in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. The sensitivity of detection was as low as 20 bacterial cells/ml of allantoic or amniotic fluids. Detection of P. multocida in dead embryos by PCR was possible up to 6 and 30 days or more following storage of dead embryos at 37 degrees C, and at 4 degrees C as well as at -20 degrees C, respectively. The study revealed that PCR assays could be employed directly for detection and confirmation of P. multocida infection in experimentally infected chicken embryos.Item Detection of pathogenic leptospires in animals by PCR based on lipL21 and lipL32 genes.(2007-06-26) Cheemaa, P S; Srivastava, S K; Amutha, R; Singh, S; Singh, H; Sandey, MEfficacy of primers capable of amplifying conserved outer membrane protein (OMP) genes i.e., lipL21 and lipL32 of Leptospira strains was tested for rapid and early diagnosis of the leptospirosis using a polymerase chain reaction (PCR). These OMP genes were found to be conserved in various leptospiral serovars viz., Canicola, Pomona, Icterohaemorrhagiae, Pyrogenes, Sejroe, Grippotyphosa, Ballum and Tarassovi as PCR products of 561 bp and 756 bp were obtained by PCR employing lipL21 and lipL32 based primers, respectively, in all these serovars. Absence of such amplicons in DNA extracted from Pasteurella, Campylobacter and Brucella confirmed the specificity of the primers. Serum and tissue samples collected from cattle, buffaloes and experimentally infected guinea pigs and calves were subjected to PCR using above primers as well as conventionally used primers G1/G2. All the sera and tissue samples, whether field samples or collected from experimentally infected animals, found positive for G1/G2 specific PCR were also positive for lipL21 and lipL32 specific PCR. The present study indicated that lipL21 and lipL32 based primers could be used for PCR based diagnosis of leptospirosis. Since G1/G2 primers are known not to amplify the DNA of Grippotyphosa, the use of primers employed in the present study could have an additional advantage in detection of cases of the disease.Item Devaluation of public teaching hospitals.(1992-07-01) Srivastava, S KItem Editing a medical book.(1994-07-01) Srivastava, S KItem Effect of cimetidine and trimipramine on gastric acid secretion in cases of acid peptic disease.(1986-06-01) Mangal, B D; Srivastava, S K; Srivastava, D K; Seth, O NItem Effect of tyrosine & iodine in experimental colloid goitre in the rat.(1979-07-01) Srivastava, S KItem Endocrine regulation of calcium and phosphate in rat eye lens and its significance in cataract formation.(1990-04-01) Srivastava, V K; Srivastava, S K; Garg, M; Chaturvedi, N; Afaq, Z; Seth, N MParathyroid hormone (PTH), calcitonin (CT)and calciferol (Vit. D3) operate synchronously to maintain a balance between calcium and phosphate levels in serum. An aberration of specific steps in the homeostatic process results in hypo/hyper phosphatemia. These aberrations may eventually lead to several diseased states. PTH and Vit. D3 induced hypercalcemia can, however, be significantly inhibited by calcitonin (CT). These findings have been correlated with the levels of calcium and phosphate obtained from human senile cataractous lenses of cortical and nuclear types. The comparison of the results indicate that amongst these three hormones PTH is most vulnerable in leading towards conditions for possible cataract formation in rat lens.Item Evaluation of recombinant Leptospira interrogans serovar canicola outer membrane proteins as diagnostic antigen.(2006-10-23) Srivastava, S K; Chaudhuri, P; Thangapandian, E; Mariya, R; Amutha, R
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