Browsing by Author "Sood, S K"
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Item Anaemia of chronic renal failure.(1979-07-01) Agarwal, S; Mathur, M; Malhotra, K K; Das, K C; Sood, S KItem Anaemia of chronic renal failure; effect of uraemic serum on proliferation and differentiation of stem cells.(1979-10-01) Agarwal, S; Mathur, M; Deo, M G; Sood, S KItem Anaemia of pregnancy in Northern India. (Delhi area).(1967-06-01) Sidhu, G S; Sood, S K; Ramalingaswami, VItem Anthropometric profile of the pre-school children of Punjab.(1976-12-01) Naik, P A; Zopf, T E; Kakar, D N; Singh, M; Sood, S KItem Beta-thalassaemia mutations in northern India (Delhi).(1998-03-26) Madan, N; Sharma, S; Rusia, U; Sen, S; Sood, S KThe present study was undertaken to define beta-thalassaemia mutations prevalent in northern India (Delhi). Forty six children of beta-thalassaemia major and their families were investigated. DNA was extracted from leucocytes and screened for mutations prevalent in the Indian population. These mutations included 619bp deletion, IVS 1-1 (G-T), IVS 1-5 (G-C), frameshift mutations FS 8/9 (+G), FS 41/42 (-CTTT), Codon 16(-C), Codon 15 (G-A), codon 30 (G-C), IVS 1-110 (G-A) and -88 (C-T). 619 bp deletion mutation was detected directly by amplification of DNA by PCR followed by agarose gel electrophoresis. Other mutations were studied by DNA amplification and dot blot hybridization using synthetic normal and mutant oligonucleotide probes labelled at 5' end with gamma-32 P-ATP. Five mutations accounted for all the chromosomes in 46 patients. 619 bp deletion mutation was found to be the commonest mutation (34.8%) followed by IVS 1-5 (G-C) in 22.8 per cent, IVS 1-1 (G-T) in 19.6 per cent, FS 8/9 (+G) in 13 per cent and FS 41/42 (-CTTT) in 9.8 per cent. Nineteen (41.3%) patients were homozygous and 27 (58.7%) double heterozygous for different beta-thalassaemia mutations. This observation of limited number of mutations is significant and will be useful in planning strategies for prenatal diagnosis of beta-thalassaemia in northern India.Item Beta-thalassaemia syndromes as seen in Northern India.(1980-03-01) Trehan, P; Madan, N; Ghai, O P; Sood, S KItem Clinical and biochemical evaluation of low protein diet in chronic renal failure in India.(1976-10-01) Malhotra, K K; Dua, S; Sood, S K; Deo, M GItem Comparison of protoporphyrin haem ratio in capillary & venous blood.(1986-11-01) Singh, U R; Rusia, U; Madan, N; Sood, S KItem Computer based application software for histopathological reporting system.(1991-10-01) Bansal, A K; Tickoo, S K; Indrayan, A; Agarwal, S; Sood, S KFor better follow up of patient and the immediate retrieval of records, we have developed a computer based application software for histopathological reporting system (HIPRIS). With its help, among others, we can (i) retrieve the biopsy report of a patient from the accession number of the specimen; (ii) find out the number of cases for a particular period as well as can analyse cases by any relevant referral parameter like department, specialty and disease and (iii) find out the time gap between receiving the specimen and reporting of result. Our experience suggests that this system greatly improves the efficiency of the histopathological laboratory.Item Congenital adrenal hyperplasia with hypoglycaemia.(1984-05-01) Verma, M; Ahmed, N; Sood, S KItem Detection of iron deficiency in pregnancy by haemoglobin, serum ferritin & protoporphyrin/haem ratio.(1988-03-01) Madan, N; Rusia, U; Manocha, A; Sood, S KItem Development of paracetamol induced hepatocellular tolerance in albino rats.(1988-08-01) Vikas,; Bhatia, A; Sood, S KItem Diamond-Blackfan anaemia (congenital pure red cell aplasia?)--a rare entity.(1990-01-01) Sharma, S; Madan, N; Khandpur, S C; Sood, S KItem Effect of leukaemic sera & cell-extracts on splenic colony counts (CFU-S).(1991-08-01) Gupta, S; Rusia, U; Agarwal, S; Sood, S KSera and leukaemic cell extracts from patients of acute leukaemia were evaluated for their effect on the repopulating ability of the pluripotent stem cells and erythroid differentiation by an in vivo splenic colony count (CFU-S) technique. Normal donor marrow cells of mice were treated with sera and cell extracts from patients of acute leukaemic and healthy controls and injected in the recipient mice. The CFU-S performed on the seventh day to assess repopulating ability of the stem cell showed consistently lower CFU-S counts in the test groups, with leukaemic sera (P less than 0.01) as well as leukaemic cell-extracts (P less than 0.001). The erythroid differentiation assessed by 59Fe uptake by the spleens also showed significantly reduced counts in the two test groups (P less than 0.01 and less than 0.001 respectively). The results indicate that both leukaemic sera and cell-extracts exert a significant suppressive effect on the repopulating ability of the stem cells and on their erythroid differentiation.Item Effect of maternal iron deficiency anaemia on foetal outcome.(1995-07-01) Rusia, U; Madan, N; Agarwal, N; Sikka, M; Sood, S KOne hundred and two pregnant women and their neonates were examined to evaluate the effect of maternal haemoglobin concentration (Hb. conc) and iron deficiency anaemia on the placental weight and the foetal outcome. Haematological and serum ferritin values were determined. It was observed that 34.3% of the pregnant women were anaemic. Maternal Hb conc. and serum ferritin showed a highly significant correlation (r = 0.40, p < 0.001) indicating that iron deficiency was the most important cause of anaemia amongst them. The maternal Hb conc. showed a significant correlation with placental weight (p < 0.05), birth weight (p < 0.01), Apgar score (p < 0.001) and birth asphyxia. Maternal serum ferritin also correlated positively with cord ferritin (p < 0.001). The study did not reveal any association between high Hb and adverse foetal outcome.Item The effect of serum from patients of chronic renal failure on colony forming units (CFU-S) in mice.(1990-04-01) Srivastava, A; Agarwal, S; Malhotra, K K; Sood, S KRepopulating ability of mouse bone marrow stem cells, treated with pre-dialysis, post-dialysis and control sera was assessed by colony forming units (CFU-S). Significant lower colony counts were observed in pre-dialysis group as compared to control group. There was an improvement in the colony counts when the cells were treated with post-dialysis sera. The study suggests the presence of inhibitor/s of CFU-S in the uraemic sera which is/are partially removed by haemodialysis.Item Erythrocyte T-activation: clinical importance & recognition by a simple routine technique.(1994-01-01) Sethi, S; Dhingra, N; Madan, N; Sood, S KErythrocyte T-activation is reported in association with bacterial infections. Although it is an unfrequent phenomenon, it has been reported in cases of septicaemia and necrotizing enterocolitis. It is important to recognize these cases as, transfusion of blood & blood products and lead to haemolytic--transfusion reactions. Here we report a case of T-activation detected during routine immunohaematological procedure in the blood transfusion laboratory. This also emphasizes the role of a routine, cost-effective test to diagnose cases of T-activation.Item Erythropoiesis in protein deficiency: a ferrokinetic study in male albino rats.(1981-06-01) Singhal, V; Sood, S K; Mathur, MItem ESR and iron status in pregnancy.(1997-10-28) Madan, N; Kapoor, S; Rusia, U; Sharma, S; Nayyar, V L; Sundaram, K R; Sood, S KESR (Westergen) correlated significantly with the iron status (as measured by Hb concentration, haematocrit, red cell count, MCH, P/H ratio, serum iron, TIBC and percent saturation of transferrin) in a group of pregnant women (PW) at term. Serum ferritin correlated negatively with the ESR but the correlation was not statistically significant. Serum ferritin levels of < 50 micrograms/L were present in 9 (34.6%) PW with ESR > or = 50 mm 1st hour and 5 (19.2%) PW with ESR < 50 mm 1st hour. The mean ESR in PW was 55.7 (+/- 22.9) and was > or = 50 mm 1st hour in 50% and < 75 mm 1st hour in 82.7%. The difference in the mean ESR in anaemic and nonanaemic PW was highly significant (p < 0.001), 87.5% anaemic PW with serum ferritin > 50 micrograms/L had ESR > or = 50 mm 1st hour, suggesting the possible effect of chronic infection in raising ferritin levels in these PW.Item Frequency of β-thalassemia trait and other hemoglobinopathies in northern and western India.(2010-01) Madan, Nishi; Sharma, Satendra; Sood, S K; Colah, Roshan; Bhatia, H M; ChildINTRODUCTION: India is an ethnically diverse country with an approximate population of 1.2 billion. The frequency of beta-thalassemia trait (βTT) has variously been reported from <1% to 17% and an average of 3.3%. Most of these studies have been carried out on small population groups and some have been based on hospital-based patients. There is also a variation in the prevalence of hemoglobinopathies in different regions and population groups in the country. A high frequency of Hb D has been reported from the North in the Punjabi population, Hb E in the eastern region of India and Hb S is mainly reported from populations of tribal origin from different parts of the country. OBJECTIVES: To study the gene frequency of βTT and other hemoglobinopathies in three regions East (Kolkata), West (Mumbai) and North (Delhi) in larghe population group (schoolchildren) for a more accurate assessment of gene frequency for planning of control programmes for haemoglobinopathies. MATERIALS AND METHODS: This study included 5408 children from 11 schools in Delhi, 5682 from 75 schools in Mumbai and 957 schoolchildren from Kolkata who were screened for βTT and haemoglobinopathies. These included 5684 children from 75 schools in Mumbai and 5408 children from 11 schools in Delhi. Children were 11-18 years of age of both sexes. The final report is, however, only on 11090 schoolchildren from Mumbai and Delhi as data from Kolkata was restricted both in numbers and objectives and could not be included for comparison. RESULTS: The overall gene frequency of βTT in Mumbai and Delhi was 4.05% being 2.68% and 5.47% in children of the two cities respectively. In Mumbai, the gene frequency was evenly distributed. Majority of the children with βTT from Mumbai were from Marathi (38.9%) and Gujarati (25%) speaking groups. Gene frequency was >5% in Bhatias, Khatris, Lohanas and Schedule Castes. In Delhi, a higher incidence was observed in schoolchildren of North and West Delhi (5.8-9.2%). The schoolchildren of North and West Delhi comprised predominantly of Punjabi origin compared to children in the South of the city (2.2%, 2.3%). When analyzed state-wise, the highest incidence was observed in children of Punjabi origin (7.6%) and was >4% from several other states. Majority of the traits from Mumbai were anemic (95.1% male and 85.6% in female). The prevalence of anemia was lower (62.7% male and 58.4% female) children with βTT from Delhi. This was a reflection of the higher prevalence of anemia in children without hemoglobinopathy in Mumbai than in Delhi. Nutritional deficiency was probably more severe and rampant in children Mumbai. Gene frequency of Hb D was greater in schoolchildren from Delhi (1.1%) than in Mumbai (0.7%). Hb S trait (0.2%) was observed exclusively in children from Mumbai. A low incidence of Hb E trait (0.04%) was seen in children in Mumbai. A higher incidence is reported from the East. The number of cases studied from the eastern region was small as the data from the East (Kolkata) could not be included in the analysis. CONCLUSION: This study comprises a larger number of children studied for the gene frequency of βTT and other hemoglobinopathies from India. Population groups with higher gene frequencies require screening programmes and facilities for antenatal diagnosis as well as increased awareness and educational programmes to control the birth of thalassemic homozygotes. The overall carrier frequency of βTT was 4.05% and reinforces the differential frequency of β-thalassemia trait in schoolchildren from Delhi and Mumbai and the higher incidence of hemoglobin D in Punjabis as reported previously. The birth incidence calculated thereof for homozygous thalassemics would be 11,316 per year which are added each year to the existing load of homozygous thalassemics. This is much higher than the previously reported number of births annually. Hence suitable control measures need to be undertaken urgently in India.