Browsing by Author "Somayaji, S N"
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Item Effect of intracerebroventricular methotrexate on brain amines.(2005-10-04) Madhyastha, S; Somayaji, S N; Rao, M S; Nalini, K; Bairy, K LIntrathecal methotrexate in children with leukemia is known to cause seizures, dementia, leukoencephalopathy and cognitive dysfunction. To investigate the role of brain amines in cognitive dysfunction, male Wistar rats were given multiple intracerebroventricular injections of methotrexate. Our earlier studies in this regard revealed disruption of brain monoamines in hippocampus with severe cytotoxic effect on CA4 hippocampal neurons. Further extending this study, the levels of brain monoamines in frontal cortex, hypothalamus and brainstem were estimated by HPLC method and histopathological study of the frontal cortex. The concentration of all three-brain amine (norepinephrine, dopamine and serotonin) levels was reduced in 2 mg/kg dose of methotrexate in frontal cortex and brain stem. Hypothalamus did not show any significant change in brain monoamine levels. No structural changes in the frontal cortex neurons were observed. Disruption of brain monoamines has been proposed as a cause of brain dysfunction from this chemotherapy. The outcome of the study may have therapeutic implications in the management of childhood lymphoblastic leukemia.Item Effect of Ocimum sanctum Linn. on normal and dexamethasone suppressed wound healing.(2006-01-25) Udupa, S L; Shetty, Somashekar; Udupa, A L; Somayaji, S NEthanolic extract of leaves of O. sanctum was investigated for normal wound healing and dexamethasone depressed healing using incision, excision and dead space wound models in albino rats. The extract of O. sanctum significantly increased the wound breaking strength in incision wound model. The extract treated wounds were found to epithelialize faster and the rate of wound contraction was significantly increased as compared to control wounds. Significant increase in wet and dry granulation tissue weight, granulation tissue breaking strength and hydroxyproline content in dead space wound model was observed. The extract significantly decreased the antihealing activities of dexamethasone in all the wound models. The results indicated that the leaf extract promotes wound healing significantly and able to overcome the wound healing suppressing action of dexamethasone. Histological examination of granulation tissue to determine the pattern of lay-down for collagen confirmed the results.Item Effect of tolmetin and its copper complex on wound healing.(1995-03-01) Somayaji, S N; Jacob, A P; Bairy, K LDose matched comparison between copper complex of tolmetin(Tol-Cu) and tolmetin(Tol) was carried out in male albino rats bearing either sutured incision, dead space or excision wounds. Results showed that Tol significantly decreased tesile strength of incision and dead space wound. This effect was totally reversed by copper present in Tol-Cu. Tol-Cu shared the significant suppressant effect of Tol on granulation formation and its collagen content. Tol suppressed wound contraction and epithelization and copper complex of Tol antagonised the suppressant effect of Tol on wound contraction and epithelization.Item Effect of topical phenytoin on burn wound healing in rats.(2011-01) Meena, K; Mohan, A V; Sharath, B; Somayaji, S N; Bairy, K LTo evaluate the effect of phenytoin on burn wounds and to compare the effect of the combination of topical phenytoin preparation in dexamethasone treated burn wounds in rats, partial thickness thermal burn wounds were inflicted upon five groups of six rats each. Group I was assigned as control, Group II received the standard silver sulphadiazine, Group III was given topical phenytoin and Group IV received injection dexamethasone, Group V received the combination of the phenytoin and the dexamethasone. The parameters observed were epithelialization period, percentage of wound contraction and histopathological analysis as indicative of the process of healing. Phenytoin group showed significant improvement in burn wound contraction in comparison to standard silver sulphadiazine group, the combination group of topical phenytoin and dexamethasone also showed significant contraction compared to dexamethasone group. The period of epithelialization also decreased significantly in groups II, III and V. In conclusion, phenytoin promotes burn wound healing as evidenced by decrease in period of epithelialization and faster wound contraction.Item Evaluation of hepatoprotective activity of Ginkgo biloba in rats.(2002-04-26) Shenoy, K Ashok; Somayaji, S N; Bairy, K LThe mechanism of hepatoprotective effects of Ginkgo biloba (GB), an herbal preparation with wide variety of therapeutic application, on paracetamol (Pcml) induced hepatic damage in rats has been investigated. GB treatment restored the marker enzyme levels indicating the in vivo protective effects against Pcml induced liver damage both in preventive and curative aspects. GB also reversed the increased TBARS levels, and elevated the GSH content of the liver. The results obtained from the study indicate hepatoprotective nature of GB, which might be due to its ability to prevent lipid peroxidation and replenishing the gllutathione level. The effects of GB were comparable to that of silymarin.Item Evaluation of intraperitoneal vincristine in malignant peritoneal effusion.(2003-07-16) Bairy, K L; Sanath, S; Jagetia, G C; Somayaji, S N; Vidyasagar, M S; Baliga, M SThe efficacy and safety of intraperitoneal administration of vincristine sulphate was determined in mice bearing Ehrlich ascitic carcinoma. The tumor bearing animals were administered with 0.5 mg/kg body weight (b.wt) of freshly prepared vincristine sulphate intraperitoneally on day 6 after tumor transplantation followed by drug administration once daily 5 days a week consecutively. The observations regarding the survival, alteration in the volume of peritoneal fluid, increase in life span and pathological changes in the liver, kidney, gastrointestinal tract and bone tissues were made. The vincristine sulphate treatment reduced the malignant cell population significantly and there were no significant changes in the histological picture of liver, kidney, bone, except the intestine, where atropy of villi demonstrating nests and cords of uniform small round cells were observed. Our experimental data suggests that intraperitoneal administration of vincristine is beneficial in malignant peritoneal effusion.Item An experimental model to produce partial thickness burn wound.(1997-01-01) Bairy, K L; Somayaji, S N; Rao, C MSimple burn wound models are always warranted. In view of this a partial thickness reproducible 2 degrees C burn wound has been produced using hot molten wax. This model is simple, convenient, and could be used to monitor wound contraction and epithelization in burn wounds.Item Phocomelia.(1995-05-01) Somayaji, S N; Bairy, K L; Rao, U K; Jacob, P AItem Pyramid environment reduces the wound healing suppressant properties of dexamethasone in albino rats.(2003-06-23) Nayak, Surekha; Rao, S Gurumadhva; Murthy, K Dilip; Somayaji, S N; Bairy, K LWith a view to investigate the contribution and role of environment within a wooden pyramid model on the wound healing suppressant effect of dexamethasone in rats, wound breaking strength, dry weight, hydroxyproline content and histology of granulation tissue of the dead space wound were studied in rats. The results indicate that the environment within the wooden pyramid not only promotes significant wound healing but also reduces the wound healing suppressant effect of dexamethasone. Histological studies also confirmed the results.