Browsing by Author "Singla, S K"
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Item Abdominal cocoon.(1988-11-01) Jain, S K; Singla, S KItem Anticalcifying properties of Dolichos biflorus (horse gram) seeds.(1994-12-01) Peshin, A; Singla, S KIn vitro effect of D. biflorus seeds on crystallization of calcium phosphate, a major constituent of kidney stone has been undertaken. There was a marked decrease in anticalcifying activity with the maturation of seeds or post-harvest storage for 6 months. The results suggested that the inhibitors of crystallization present in seed extract of D. biflorus were water soluble, heat stable, polar, non-tannin and non-protein in nature. There were two or more different inhibitors of calcium phosphate precipitation since both the dialysate and the dialysed fractions contained inhibitory activity, though it was more in the dialysed fractions. The anticalcifying activity was lost completely with activated charcoal which was not recovered or eluted by any solvent tried.Item Anticalcifying properties of urine from normal persons and kidney stone patients.(1978-10-01) Singla, S K; Goyal, S C; Singh, K P; Jethi, R KItem Congenital intrinsic duodenal obstruction due to diaphragm.(1989-07-01) Jain, S K; Singla, S K; Taneja, S B; Pathania, O PItem Effect of nucleoside-5'-phosphates on collagen-induced in vitro mineralization.(2004-08-03) Talwar, H S; Singla, S K; Tandon, C; Jethi, R KNucleoside triphosphates (NTPs) at 4-10 microM concentrations were found to inhibit the rates of collagen-induced in vitro mineralization and ion exchange reactions. The sequential removal of the terminal phosphate groups caused a step-wise decrease in their inhibitory potency. The results suggest that NTPs inhibit the rates of ion uptake and exchange reactions at concentrations much lower than their intracellular physiological concentrations. Thus NTPs may be involved in the control of biological mineralization and the tissues which mineralize under physiological conditions develop a system to locally convert NTPs to NDPs and NMPs.Item Hand-made Cloning: A Guide for Cloning Water Buffaloes.(2014-07) Singla, S K; Raja, A; Nala, N; Chauhan, M S; Manik, R S; Palta, PThe present review is a detailed discussion on comparable benefits of hand-made cloning (HMC) technique than micro-manipulation based conventional cloning and developed in the author’s laboratory. Hand-made cloning technique does not require micromanipulators, because the manipulations required for both enucleation and nucleus transfer are performed by hand. The HMC technique includes manual bisection of zona-free oocytes and the simultaneous fusion of the somatic cell with two cytoplasts to produce a cloned embryo. The benefits of HMC include low setup costs for limited equipment, no requirement of highly trained expertise and in vitro effciency comparable to traditional somatic cell nuclear transfer technology. Embryos produced by HMC can be cryopreserved and capable of producing live births. The HMC technique is now applied to different species and can be used in large scale nuclear transfer programs.Item Hirschsprung's disease with intestinal malrotation and midgut volvulus: a rare association.(1989-07-01) Jain, S K; Singla, S K; Sharma, M; Pathania, O P; Taneja, S BItem Increased capacitation of buffalo sperm by heparin as confirmed by electron microscopy and in vitro fertilization.(1997-10-25) Chauhan, M S; Singla, S K; Manik, R S; Madan, M LCapacitation of buffalo sperm was evaluated by induced acrosome reaction (AR) upon the exposure of 10 mM Ca2+. Culture of sperm for 8 hr in BO medium supplemented with 10 micrograms/ml heparin significantly (P < 0.01) increased the percentage of AR and confirmed by transmission electron microscopy. Vesiculization of outer acrosomal membrane and plasma membrane was observed significantly higher (P < 0.01) following 8 hr of sperm culture with heparin. Culture of sperm with heparin also increased rate of fertilization of in vitro matured oocytes and their subsequent development up to morula/blastocyst stage (P < 0.01). The study demonstrates that capacitation of buffalo sperm by heparin required at least 8 hr exposure of sperm to heparin for maximum acrosome reaction.Item Micromanipulation and cloning studies on buffalo oocytes and embryos using nucleus transfer.(1997-12-06) Singla, S K; Manik, R S; Madan, M LAn investigation for testing the viability of production of cloned buffalo embryos through nucleus transfer has been made. Matured buffalo oocytes, after zona cutting to an extent of 60 degrees near polar body, were enucleated using a new approach. Instead of aspirating the cytoplasm contents in a pipette, the half of cytoplasm of oocyte was pushed out, thereby also taking away the nuclear material of the oocyte, leaving the demi-oocyte with the zona pellucida enucleated. The absence of fluorescence confirmed the success of the enucleating process. For enucleating, the oocytes which had intact plasma membrane were eligible for bisectioning. There was no significant difference in oocytes having intact membrane among grade I (33.9%) and grade II (31.4%) oocytes, whereas lower percentage of grade III oocytes had a very low percentage having intact plasma membranes (8.5%). The hours of maturation for 32, 37 and 42 did not influence the per cent oocytes which had intact membranes. All the bisected or demi-oocytes tested with fluorescence screening yielded successful enucleation in 88.2% demi-oocytes. The temporal effect of three maturation hours of 32, 37, and 42 hr; two electrical pulse numbers of 2 and 3 pulses and two magnitudes of electric pulses of 15 and 20 V were studied for their effect on the percentage of successful fusion of demi-oocyte blastomere complexes and the rate of complexes undergoing cleavages. The time period for which the oocytes were subjected to the process of maturation significantly affected the per cent fusions and per cent cleavage of the demi-oocyte blastomere complexes and 32 hr maturation yielded less fusions (38.5%) compared to maturation for 37 and 42 h (53.2 and 57.8%, respectively). The treatment of either 2 or 3 electrical pulse numbers resulted in significantly different fusion (45.6 and 54.1%) as well as cleavage rates (18.2 and 26.1%) of demi-oocyte-blastomere complexes electrofused. The treatment of two levels of magnitude of 15 and 20 V of an electric current resulted in similar per cent fusion (48.0 and 51.6%) and cleavage rates (21.0 and 23.2%). Fortified TCM with either 10 or 20% FBS for culturing freshly electrofused complexes for 1 hr did not differ significantly with respect to per cent complexes fused and cleaved, giving a fusion rate of 46.2 and 53.8% and cleavage rate of 21.2 and 23.2% for 10% and 20% FBS, respectively. Production of cloned embryos through the process of nuclear transfer has been accomplished. The successful cleavages of the nuclear transferred oocytes demonstrated the viability of enucleation procedures of the oocytes and technology implementation of electrofusion in buffalo oocytes.Item Multiple intracranial tuberculomas.(1986-08-01) Bhatnagar, D P; Kaur, K; Singla, S KItem A simple method for the study of in vitro calculogenesis.(1981-03-01) Singla, S K; Jethi, R KItem Spontaneous mesenteric hematoma.(1989-04-01) Singla, S K; Champakam, N S; Bishnoi, P K; Bajaj, P; Chopra, SItem Urolithiasis: Phytotherapy as an adjunct therapy.(2014-02) Aggarwal, A; Singla, S K; Tandon, CRole of herbal drugs and medicinal plant extracts in the successful treatment of urolithiasis, classified as the third most common urinary tract diseases is well documented. Ayurvedic plants and their components mediate antilithogenic effects by altering ionic composition of urine, being diuretic, antioxidant or having antimicrobial activity. Therapeutic peptides and proteins have unique place in pharmaceutical biotechnology due to their critical roles in cell biology. The innovation in antilithiatic proteins is that they are anionic, rich in acidic amino acids which make oxalate unavailable by interacting with calcium and have EF Hand domain which is a characteristic feature of various calcium binding protein like calgranulin, osteopontin. The review provides a background on the pathogenesis of urolithiasis and medical treatments. It focusses on the present research evaluating the scientific basis of antilithiatic potential of various plants and role of plant proteins as therapeutic agents thus opening new vista in the management of urolithiasis. Further investigations are required to fully decipher the mode of action of the potent biomolecules so as to exploit their preventive and therapeutic potential.