Browsing by Author "Singh, N."
Now showing 1 - 5 of 5
Results Per Page
Sort Options
Item Biosynthesis and characterization of silver nanoparticles using root extract of Saussurea lappa (Decne.) Clarke and their antibacterial activity(Triveni Enterprises, 2019-09) Groach, R.; Yadav, K.; Sharma, J; Singh, N.Aim: To synthesize and characterize stable silver nanoparticles (AgNPs) from the root extract of Saussurea lappa, a medicinally important plant. The AgNPs was further evaluated for their antibacterial activity. Methodology: The biosynthesised AgNPs were characterised by change in colour pattern and confirmed by UV–Visible spectroscopy, Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Results: UV-Vis spectroscopy study showed characteristic surface plasmon band (SPR) of synthesized AgNPs at 418 nm. FTIR studies revealed presence of some biomolecules responsible for reduction, stabilization and capping agents towards these synthesized AgNPs. TEM analysis revealed that the size of AgNPs ranged between 7.13 – 24.0 nm and had spherical shape. XRD data showed face-centered cubic (fcc) nature of AgNPs. The synthesized AgNPs showed significant antibacterial activity against one Gram positive (Bacillus cereus) and one Gram negative (Escherichia coli) bacterial strain. Interpretation: The biologically synthesized nanoparticles from the root extract of S. lappa were stable and showed enhanced antibacterial activity against both type of bacterial strains and could be utilized for industrial and remedial purposes.Item Bulk segregant analysis identifies SSR markers associated with Alternaria blight resistance in linseed (Linum usitatissimum L.)(Triveni Enterprises, 2019-11) Singh, N.; Kumar, R.; Kumar, S.; Singh, P.K.; Singh, B.; Kumar, U.; Khatoon, B.; Yadav, H.K.Aim: Alternaria blight caused by Alternaria lini is one of the major diseases of linseed which severely affects the yield and productivity. Here, we utilizes F2 mapping population derived from a resistant (JRF-4) and a susceptible (Chambal) genotypes of linseed and SSRs to identify the markers associated with Alternaria blight resistance using bulk segregant analysis approach. Methodology: A population consisting of 154 F2 individuals was developed from the cross between JRF-4 (resistant) and Chambal (susceptible). All 154 F2 individuals were screened with 100 polymorphic SSRs to identify extreme phenotype. Two bulk of extremes phenotypes (disease resistant and disease susceptible) from F2 mapping population were used for the bulked segregant analysis. The SSR primers that distinguished the parental lines were used to amplify the DNA from two bulks and banding pattern was observed to identify the SSRs that can differentiate the resistant and susceptible phenotypes bulk for Alternaria blight. Markers validation was carried out by amplifying DNA from individual plants of each bulk. Results: Out of 100, only 10 markers showed polymorphism among the bulks and of which only three markers viz., LUSc 898_3_12, Lu 2472 and Lu 3078 were able to differentiate the disease resistant and susceptible individuals from F2 population. Further, single marker linear regression approach was used to validate the association of selected polymorphic markers with the disease. The markers LUSc 898_3_12 and Lu 2472 showed significant regression which confirmed their linkage with Alternaria blight resistance. Interpretation: The two markers having significant regression can be used for diseases resistance breeding during marker assisted selection.Item Development and Organoleptic Evaluation of Iron Rich Pancake Premix Using Spirulina and Super Flours(Sciencedomain International, 2023-06) Srivastava, J.; Singh, N.; Nanda, A.Since ancient times, poor nutrition has been the primary culprit of iron deficiency, especially in the school going adolescent girls. There may be a number of factors that contribute to this. The production of affordable, wholesome, and practical foods is a challenge for the food industry. Spirulina platensis, a microscopic and filamentous cyanobacterium is a blue-green algae that is considered to be one of the ancient life forms on Earth. To increase the nutritive value and to provide other vital nutrients, best wholesome cereals must be chosen over or in combination with refined flour. Here, quinoas, soy flour and amaranth come in the picture. The preparation of pancake premix and the final product was done in the department of food and nutrition, school of home sciences , BBAU Lucknow in the duration of nine months from September 2023 to May 2023. The assessment was successful and yielded genuine beneficial outcomes. The spirulina pan cakes excel in terms of flavour, aroma, and mouth feel. The panellists found the pancake's green tint to be somewhat objectionable.Item Knowledge, Attitude and Practice towards COVID-19 Vaccination in District Badaun: A cross-sectional study(Department of Community Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi- 221005, India., 2022-06) Prasot, R. M.; Ibrahim, M.; Singh, N.; Ahmad, M. F.; Bihari, A.; Singh, R.Background: COVID-19 was declared pandemic by WHO on 30th January 2020 and to cure it, No specific antiviral treatment has been developed yet, therefore only preventive measures such as; facemask, regular hand washing, social as well as physical distancing, respiratory etiquettes and vaccination against Covid-19, are proven methods of its control and prevention. Objectives: To study the knowledge, Attitude and Practices among people about COVID-19 vaccine and find out various socio-demographic factors for its decision making. Material &Methods: A descriptive cross sectional study was conducted among the persons attended the tertiary care center to get vaccine against COVID-19 at Government Medical College, Badaun, UP. Results: Out of all respondents, majority (77.2%) of them accepted to get the vaccine as soon as available. 81.5% of respondents were male and more than half (60%) of them were unmarried. More than three fourth (77.5%) of respondents were unemployed and nearly one third (32%) belonged to BPL category. As per study, majority of respondents (86%) and (71.8%) said that vaccine is safe and effective way to control and prevent COVID-19, respectively.(77.2%)respondents who accepted that vaccine should be taken as soon as available, more than half (57%) of them said that doctor's recommendation is an important factor in vaccination decision-making.Conclusion: The most important factor for vaccine hesitancy is the occurrence of mild to moderate adverse effects following immunization, and this may be the biggest challenge in the global response against the Covid-19 pandemic.Item Prevalence of Depression among Geriatric Population in Old Age Homes of South Delhi(Medsci Publications, 2022-03) Singh, A; Mishra, CM; Chellaiyan, VG; Verma, JK; Sasithra; Singh, N.Background: Ageing population is increasing globally year by year. Around 15% of adults aged 60 and above suffer from a mental disorder and depression being the commonest among them. Geriatric mental health and physical condition are more important for perceiving good quality of life. Materials and methods: A cross-sectional study was conducted in three old age homes of South Delhi. Data was collected using a structured, pre-tested, validated questionnaire - Geriatric Depression Scale (GDS). After obtaining consent from the participants, the required number of samples (n=105) were collected through systematic random sampling method. Statistical analysis was done using SPSS v21. Results: Overall prevalence of depression was found to be 73.3%. Of which 26.7% had mild depression, 31.4% had moderate depression and 41.9% had severe depression respectively. Statistical significance (p<0.05) was found between married, illiterate, female by gender, those with no regular income, those who had associated co-morbidities, participants whose family/friends did not visit regularly and depression. Conclusion: Increased prevalence of depression among the geriatric population residing in old ages indicates the growing mental health burden and the need for it to be identified at early stage and treated with proper therapeutic measures.