Browsing by Author "Singh, Manoj Kumar"

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    Bioactivity guided fractionation of Moringa oleifera Lam. flower targeting Leishmania donovani.
    (2015-11) Singh, Manoj Kumar; Paul, Joydeep; De, Tripti; Chakraborti, Tapati
    Leishmaniases is a group of diseases caused by the protozoan parasite belonging to the genus Leishmania. At least 20 species of Leishmania are known to infect humans transmitted by female sandflies, Phlebotomus spp. Leishmania donovani causes visceral leishmaniasis, considered most lethal among the common three forms of leishmaniasis. Lack of appropriate vaccines, emergence of drug resistance and side effects of currently used drugs stress the need for better alternative drugs, particularly from natural sources. Here, we conducted in vitro and in vivo experiments to study the efficacy of different parts of Moringa oleifera Lam. against Leishmania donovani promastigotes. The flower extract of M. oliefera (MoF) was found to be the most potent antileishmanial agent when compared to other parts of the plant like leaf, root, bark and stem. It imparted significant reduction in parasite number in infected macrophages. The bioactivity guided fractionation of MoF showed ethyl acetate fraction (MoE) as the most active and gave significant parasite reduction in the infected macrophages. Further, growth kinetics studies revealed loss of L. donovani promastigotes viability in the presence of MoE in both time and dose dependent manner. In vivo experiment in Balb/c mouse model of leishmaniasis supported the in vitro findings with a remarkable reduction of the parasite burden in both liver and spleen.
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    Differential toxicological endpoints of di(2-ethylhexyl) phthalate (DEHP) exposure in MCF-7 and MDA-MB-231 cell lines: Possible estrogen receptor α (ERα) independent modulations.
    (2014-11) Das, Mihir Tanay; Singh, Manoj Kumar; Thakur, Indu Shekhar
    Wide spread use of Di-(2-ethylhexyl) phthalate (DEHP) has made it a ubiquitous contaminant in today’s environment, responsible for possible carcinogenic and endocrine disrupting effects. In the present investigation an integrative toxico-proteomic approach was made to study the estrogenic potential of DEHP. In vitro experiments carried out with DEHP (0.1-100 μM) induced proliferations (E-screen assay) in human estrogen receptors-α (ERα) positive MCF-7 and ERα negative MDA-MB-231 breast cancer cells irrespective of their ERα status. Further, DEHP suppressed tamoxifen (a potent anti-breast cancer drug) induced apoptosis in both cell types as shown by flowcytometric cell cycle analysis. Label-free quantitative proteomics analysis of the cell secretome of both the cell lines indicated a wide array of stress related, structural and receptor binding proteins that were affected due to DEHP exposure. The secretome of DEHP treated MCF-7 cells revealed the down regulation of lactotransferrin, an ERα responsive iron transport protein. The results indicated that toxicological effects of DEHP did not follow an ERα signaling pathway. However, the differential effects in MCF-7 and MDA-MB-231 cell lines indicate that ERα might have an indirect modulating effect on DEHP induced toxicity.
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    The Effect of Integrated Nutrient Management on Various Growth Parameters of Cluster Bean (Cyamopsis tetragonoloba L.)
    (Ms. M. B. Mondal, 2023-10) Yashraj; Singh, Manoj Kumar; Katiyar, Hariom; Tyagi, Vikash; Vinayak; Tomar, Harshit; Singh, Jagraj; Kumar, Rupesh
    The present investigation entitled “The effect of integrated nutrient management on various growth parameters of cluster bean (Cyamopsis tetragonoloba L.)” was carried out at Horticultural Research Centre of Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut – 250110 during 2022-23. In this study integrated nutrient management (INM), farmyard manure (FYM), vermicompost and phosphate solubilizing bacteria (PSB) were used with different combinations. The entitled experiment was conducted in Randomized Block Design (RBD) with three replications. Application of INM dose @ 75% RDF + Vermicompost (1.5 t ha-1) + Rhizobium (2 kg ha-1) + PSB (1 kg ha-1) was found to be the most effective in terms of growth parameters viz., plant height (89.90 cm), number of branches plant-1(9.73), number of clusters plant-1(27.83) and number of leaves plant-1(37.98). Therefore, it can be suggested that a dose of 75% RDF + Vermicompost (1.5 t ha-1) + Rhizobium (2kg ha-1) + PSB (1 kg ha-1) suitable for the commercial cultivation of vegetable cluster bean in the Western Plane Zone of Uttar Pradesh.
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    Effect of Integrated Nutrients Management Practices on Growth Parameters of Pointed Gourd (Trichosanthes dioica)
    (Ms. M. B. Mondal, 2023-08) Kumar, Sandesh; Kumar, Vipin; Prakash, Satya; Singh, Manoj Kumar; Kumar, Arvind; Singh, S. P.; Kumar, Deepak; Gangwar, Vishal
    Introduction and Aim: Pointed gourd (Trichosanthes dioica Roxb) is one of the most profitable and nutrient-rich summer crops. The present investigation entitled “Effect of Integrated Nutrients Management Practices on Growth and Yield of Pointed gourd (Trichosanthes dioica) cv. Papdi & Rudra’’ was carried and conducted at open field condition located at Horticulture Research Centre (HRC), at Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut U.P India during Kharif season of 2022-2023.Methods: The experiment was carried out in randomized block design keeping two varieties i.e. Papdi and Rudra with three replications. The experimental trial consists 10 different treatment.Discussion and Conclusion: All the treatments showed significant differences for most of the traits under study, the combination of T9 (Vermicompost @ 10 ton /hac + Jivamrit @8% Spray) followed by the treatment T10 (F.Y.M @ 15 ton/ha + Jivamrita @ 8% sprays) was best suited for the growth attributes of pointed gourd.: Hence, the above treatment can be suggested as a combination for getting higher growth of pointed gourd.
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    Effect of physico-chemical treatments on ripening behavior and post- harvest quality of Amrapali mango (Mangifera indica L.) during storage.
    (2012-03) Singh, Pankaj; Singh, Manoj Kumar; Kumar, Vipin; Kumar, Mukesh; Malik, Sunil
    An experiment was done to assess the effect of various physico-chemical treatments on ripening behavior and post harvest quality of mango cv. Amrapali. The experiment was planned under completely randomized design (CRD ) with three replications. The treatment units was five fruits per replication. Total 14 treatments were applied. Out of these, ethrel 750 ppm treated fruits showed better results in respect of specific gravity (0.88), moisture loss (8.45%), decay (2.5%), total soluble solids (TSS, 20.7o brix), sugar content (14.39%) and acidity content (0.32) followed by ethrel 500 ppm; specific gravity (0.90), moisture loss (8.82%), decay (3.5%), TSS (20.7o brix ), sugar content (13.99%) and acidity content (0.36%). The pedicellate fruits and ethrel+bavistin (750+1000 ppm) were also found to be significantly superior over control in respect of specific gravity (0.88 and 0.86), moisture loss (9.10 and 9.33%), decay (4.0 and 5.33%), TSS (20.1 and 20.4o brix), sugar content (12.70 and 12.80%) and acidity content (0.42 and 0.38%), respectively. Based on results of this study, it can be concluded that ethrel 750 ppm was found to be the most suitable treatment in improving physico-chemical traits i.e. ripening, storage, quality and shelf-life for commercial purpose in mango.
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    Growth factors and culture media dependent in vitro expansion and characteristics of enriched spermatogonial stem cells derived from adult caprine testis
    (The CSIR-National Institute of Science Communication and Policy Research (CSIR-NIScPR) (formerly known as NISCAIR)., 2025-05) Pathak, Manisha; Singh, Shiva Pratap; Pathak, Juhi; Goel, Anjana; Soni, Yogesh Kumar; Singh, Manoj Kumar
    In vitro expansion of spermatogonial stem cells (SSCs) has gained significant attention, as it offers a promising alternative for preserving and utilizing these cells beyond their natural regenerative capacity. Present study aimed to investigate the effect of supplementation of growth factors in culture media and its comparison with commercially available media for in vitro expansion and maintenance of culture characteristics of adult caprine SSCs (cSSCs). In Trial-1, cSSCs were isolated from adult goats and an enriched population of cSSC was randomly divided into 5 groups i.e., group-1 (control; no growth factor), group-2 (GDNF; 40 ng/mL), group-3 (FGF2; 10 ng/mL), group-4 (EGF; 5 ng/mL) and group-5 (GDNF+FGF2+EGF). Following cultivation of cSSCs, morphological assessment, colony counting, and expression of alkaline phosphatase (ALP) and PGP9.5 were conducted and results were compared among the groups. Further, in Trial-2, the performance of optimized cSSCs culture media (in-house media) was compared with 5 commercial media viz, ?-MEM, MesenPRO RSTM, StemPRO®-34, Stemline®, and Ham's F-12 Nutrient Mix for improved growth and culture characteristics of cSSCs. The cluster-forming activity (CFA) assay, ALP staining, morphological evaluation of cSSCs colonies, and expression analyses of marker genes were performed. In Trial-1, the total number of colonies, size of colonies, and ALP expression were significantly (P<0.05) higher in group-5 compared with other groups. In Trial 2, the in-house media produced significantly (P<0.05) higher number and larger cSSC colonies among all the media tested. Similar results were observed in CFA and ALP staining. The results of expression analyses demonstrate upregulation of pluripotency (PGP9.5 and PLZF) and adhesion (E-cadherin) marker genes, and downregulation of apoptosis marker gene (BCL-6) in the cells when grown in in-house media. Overall, our results demonstrate that in-house media, with a combination of growth factors, provides a more favorable niche for proliferation, colony formation, and maintenance of functional characteristics of adult cSSCs in the in vitro culture systems. These results can be utilized for future studies and application that require optimum expansion of cSSCs or other stem cells.
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    Laparoscopic Paediatric Hernia Repair Using Tuohy Needle - Our Preliminary Experience of 100 Cases
    (Akshantala Enterprises Private Limited, 2020-09) Bhattacharjee, Siddhartha Sankar; Daniel, Sneha; Das, Prasenjit; Hussain, Khorshid Alam; Kumar, Nitesh; Singh, Manoj Kumar
    Congenital inguinal hernia repair is one of the most commonly encountered paediatric surgical procedures. With advancements in minimal access surgery, there is an increasing inclination towards the repair of paediatric inguinal hernia (PIH) laparoscopically over an open procedure. Currently, a single port laparoscopic percutaneous extraperitoneal closure, a simple and reliable method, is being used widely. METHODS100 patients with PIH were treated using a single port LPEC technique from August 2014 to July 2018. The study was conducted with a modified LPEC technique using a Tuohy needle. The deep ring was encircled with the help of Tuohy needle under laparoscopic visualization and a suture was passed through it. The needle was then used to encircle the ring from the opposite side and the initial suture was retrieved with the help of a loop, and a purse string was created to obliterate the deep ring with extracorporeal knotting. RESULTS100 congenital hernias were repaired by LPEC method. Mean age was 5.85 ± 2 years. Male to female ratio was 7.3 : 1. 96% of cases were unilateral and 4 % were bilateral preoperatively. PPV was found in 8% of cases intraoperatively. Mean operating time was 22.08 ± 10.2 and 27.6 ± 11.47 minutes for unilateral and bilateral cases respectively. There were no intraoperative complications and no recurrences. Only one patient developed wound infection. CONCLUSIONSLPEC of hernial sac using a Tuohy needle is a safe and effective method for the repair of PIH. It is easy to perform, faster, and offers good cosmesis with cure for imminent contralateral hernias.
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    Morphometric study of lymphoid cells in mycosis fungoides and its simulators.
    (2003-01-19) Sinha, Arvind Kumar; Singh, Manoj Kumar; Dinda, Amit Kumar; Ramam, M
    The distinction of early stages of mycosis fungoides from benign lymphoid disorders of skin is difficult by conventional histological techniques. We studied 10 cases of mycosis fungoides, 10 cases of large plaque parapsoriasis, 10 cases of other benign lymphoid disorders of skin and 5 cases of lymph nodes. Nuclear area, perimeter of the nucleus, nuclear contour index, cytoplasmic area, form factor and nuclear cytoplasmic ratio as well as DNA-ploidy were determined by image analysis. There were statistically significant difference (P value < 0.05) between all parameters except nuclear cytoplasmic ratio of the lymphoid cells of Mycosis Fungoides and benign lymphoid disorders of skin. Aneuploidy was found in 50% cases of Mycosis Fungoides. Histopathological parameters like epidermotropism pautrier micro-abscess and atypical lymphocytic infiltrate in both epidermis and dermis were more marked in aneuploid than diploid cases. So, the determination of nuclear contour index and DNA-ploidy is of importance to differentiate between Mycosis Fungoides and benign lymphoid disorders of skin.
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    Ochronosis with subtle histological findings.
    (2015-11) Vallonthaiel, Archana George; Kakkar, Aanchal; Singh, Manoj Kumar; Ramam, M