Browsing by Author "Sharma, Sanjeev Kumar"
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Item Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease.(2009-09) Sasikala, Mitnala; Reddy, D Nageshwar; Pratap, Nitesh; Sharma, Sanjeev Kumar; Reddy, P Balkumar; Sekaran, Anuradha; Banerjee, Rupa; Reddy, D BhaskaraBackground and Objective The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn’s disease (CD) remains controversial, because of conflicting reports demonstrating the presence of MAP-specific insertion sequence from intestinal biopsy tissues of patients clinically diagnosed for the disease. The present study was carried out to investigate the presence of MAP DNA in the intestinal tissues of CD patients to ascertain the relevance of MAP in Indian patients with CD. Methods Patients diagnosed as CD at our institute were recruited. Healthy individuals without inflammatory bowel disease served as controls. Mucosal biopsy specimens were collected from ileum and colon in duplicates and subjected to histopathological examination and polymerase chain reaction (PCR) amplification. Total DNA (81 CD patients, 85 healthy individuals) and total RNA (12 CD patients, 12 healthy individuals) isolated from tissue specimens was used for amplification of MAP-specific IS900 by nested PCR. Results MAP-specific IS900 DNA and RNA could not be detected by nested PCR in the intestinal tissues of any patient with CD. Conclusion Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.Item Assessment of morbidity profile in urban slum community of Udaipur (Rajasthan).(2015-04) Kumar, L Dilip; Mathur, Harish Narayan; Sharma, Sanjeev KumarBackground: The urban slums has diversity, but the universal characteristics refer to overcrowding and congestion, lack of hygiene, extremely poor sanitation, lack of garbage disposal facilities, high density of insects and rodents, makes the dwellers more prone to morbidity. Objectives: To study morbidity profile in urban slum dwellers. Methods: Cross sectional study had been carried out between January 2014 to July 2014 on slum dwellers of Udaipur. Information obtained by interviewing the head and/or other family members of about 76 households. Results: The morbidity rate for both sexes was 33.0%. About 31.2% males and 34.6% females were either suffering from or have a history of one or more illness within previous two weeks. Anemia was found in 11.2% of females. Respiratory tract infections and diarrhea were present in 8.8% and 2.1% of studied population respectively. Among five years above age slum dwellers, 18.9%, 38.3% and 31.2% had a habit of smoking, tobacco chewing and alcohol intake respectively. Conclusions: Slum dwellers were unaware of the effective information on hygiene, health and substance use, education, communication activities along with effective health care delivery measures needed.Item Assessment of nutritional status in pre-school children in Teetardi village near Geetanjali hospital, Udaipur (Rajasthan).(2015-04) Sharma, Sanjeev Kumar; Mathur, Harish Narayan; Kumar, Dilip LBackground: The pre-school age group (2-5 years) was evaluated for nutritional status at Teetardi village near Geetanjali hospital Udaipur. Objectives: To study the nutritional status in pre-school children. Methods: Cross sectional study carried out during February 2014 to June 2014 with 300 children at Teetardi village near Geetanjali hospital Udaipur. Body weight, height recorded with age, gender and also interviewing the mother. Results: The age and sex distribution of 300 children in present study was 52% males and 48% females. The nutritional status in the children was determined as per World Health Organization child growth standards. Result reveals that out of total male children 4.5% were severely stunted and out of total female children 4.2% were severely stunted. Out of total 10.3% of male children and 11.1% of female children were found stunted. Whereas out of total children 3.2% of male children and 4.9% of female children were severely wasted. Out of total children 10.9% of male children and 12.5% of female children were found wasted. Also 7.1% of male children and 5.5% of female children were overweight also found that 1.9% of male children and 0.7% of female children are obese. Conclusions: There is necessity of more education in reference to nutrition and dietary habits including proper antenatal care and postnatal care of mother to reduce the prevalence of nutrition related abnormalities in children.Item A cross sectional study regarding awareness of HIV/AIDS and practices of condoms among slum dwellers of Ajmer, Rajasthan, India(Medip Academy, 2019-07) Meharda, Bharat; Sharma, Sanjeev Kumar; Keswani, Mahesh; Sharma, Rakesh KumarBackground: People living in slums are often deprived of health-care facilities and it make them vulnerable to infection like HIV/AIDS. Health education and behavioural changes is pivotal for prevention of HIV and HIV-related stigma. Methods: It was community based cross sectional study carried among 288 slum dwellers of Ajmer, Rajasthan, India.Results: Out of 288 study subjects (male and females in the group of 15-54 years) 144 (50%) males and 144 (50%) females. Out of 288 study subjects 278 (96.53%) were heard about HIV/AIDS. Out of 249 study subject who were aware about condom, 107 (42.97%) study subjects [74 (56.06%) male and 33 (28.21%) female] were presently using of condom. out of 278 study subject who had ever heard about HIV/AIDS majority 215 (77.34%) subjects got the information regarding HIV/AIDS through TV/cinemaConclusions: Present study shows that the study subjects of slums at Ajmer has inadequate awareness regarding the HIV/AIDS and poor practice of condoms.Item Curcumin sensitizes lung adenocarcinoma cells to apoptosis via intracellular redox status mediated pathway.(2012-12) Kaushik, Gaurav; Kaushik, Toshi; Yadav, Subodh Kumar; Sharma, Sanjeev Kumar; Ranawat, Pavitra; Khanduja, Krishan Lal; Pathak, Chander MohanThe present study demonstrates that curcumin acts as pro-oxidant and sensitizes human lung adenocarcinoma epithelial cells (A549) to apoptosis via intracellular redox status mediated pathway. Results indicated that curcumin induced cell toxicity (light microscopy and MTT assay) and apoptosis (AnnexinV-FITC/PI labeling and caspase-3 activity) in these cells. These events seem to be mediated through generation of reactive oxygen species (ROS) and superoxide radicals (SOR) and enhanced levels of lipid peroxidation. These changes were accompanied by increase in oxidized glutathione (GSSG), reduced glutathione (GSH) and -glutamylcysteine synthetase (-GCS) activity, but decrease in GSH/GSSG ratio. The induction of apoptosis and decrease in GSH/GSSG ratio was also accompanied by sustained phosphorylation and activation of p38 mitogen activated protein kinase (MAPK). On the other hand, addition of N-acetyl cysteine (NAC), an antioxidant, blocked the curcumin-induced ROS production and rescued malignant cells from curcumin-induced apoptosis through caspase-3 deactivation. However, L-buthionine sulfoximine (BSO), a GSH synthesis blocking agent, further enhanced curcumin-induced ROS production and apoptosis in A549 cells. Decreased GSH/GSSG ratio seems to be a crucial factor for the activation of MAPK signaling cascade by curcumin. The study therefore, provides an insight into the molecular mechanism involved in sensitization of lung adenocarcinoma cells to apoptosis by curcumin.Item What a Clinical Hematologist should Know about B Cells?(Sciencedomain International, 2022-01) Sharma, Sanjeev KumarB cells are very crucial part of our immune system. They protect us from various infections by secreting antigen-specific antibodies, which neutralize the infectious agents. It is important for a clinical hematologist to know about the B cell development, function and the diseases developing from the quantitative or qualitative defects in B cells. This simple and short review is focused on the basic understanding and clinical hematologist’s perspective of B cells.