IMSEAR Repository :: Browsing by Author "Sharma, Preeti"

Browsing by Author "Sharma, Preeti"

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Alkaline pretreatment optimization for Pinus roxburghii needle biomass employing response surface methodology for bioethanol production by separate hydrolysis and cofermentation
(NISCAIR-CSIR, India, 2019-11) Slathia, Parvez Singh; Raina, Neelu; Bhagat, Deepali; Raina, Vinaik; Sharma, Nisha; Sharma, Preeti
Production of bioethanol from lignocellulosics can cater to the supply of renewable fuel to transport sector besides being environment friendly. In the present work, bioethanol production potential of Pinus roxburghii needle biomass (PNB) has been studied by optimizing the thermochemical pretreatment method using alkaline conditions (NaOH) firstly by one variable at a time (OVAT) approach, followed by Response Surface Methodology (RSM) with central composite design (CCD) tool. Total reducing sugar (TRS) yield was enhanced from 22.4 g/L (OVAT) to 32.4 g/L using design of experiment (DoE) approach. Effect of pretreatment on PNB was studied by FTIR, phloroglucinol staining and estimation of phenolics which indicated lignin removal. Enzymatic hydrolysis was done by the action of commercial enzymes cellulase and pectinase with loading of 5U/g biomass. The TRS yield was further enhanced to 67.95 g/L after enzymatic hydrolysis. Using separate hydrolysis and cofermentation (SHCF) approach for fermentation of PNB hydrolysate, 28.75 g/L bioethanol was obtained when combined cultures of Saccharomyces cerevisiae (MTCC-36) and Pichia stipitis (NCIM-3498) were used.
Assessment of Depression in Patients of Type-2 Diabetes Mellitus Attending a Tertiary Care Centre
(International Society for Contemporary Medical Research, 2019-06) Sharma, Preeti; Kumar, Santosh; Sharma, C.S.; Dixit, Vidhata; Rathi, Harsh; Arya, Viddur
Introduction: Type-2 diabetes mellitus is a common, chronic metabolic disorder with multiple complications if not well controlled. Depression is a very common psychiatric comorbidity in these patients. Multiple environmental and patient related factors are linked with this co-morbidity. It is important to address depression and related factors in these patients for a better outcome. This study aimed to assess depression and distribution of various socio-demographic and clinical details in patients of type-2 diabetes mellitus. Material and Methods: Using a purposive sampling technique, a total of 118 patients of type-2 diabetes mellitus who fulfilled the inclusion and exclusion criteria of this study were enrolled. They were administered Hamilton Depression Rating Scale to assess depression. Overall 66.1% of the patients had co-morbidity of depression. Results: Significantly more number of patients of type-2 diabetes mellitus with co-morbid depression were unmarried (41% vs 15%, χ2=9.029, df=2, p<.05), unemployed/unskilled workers (21.8% vs 5% / 73.1% vs 55%, χ2=24.893, df=2, p<.01), from lower socio-economic status (71.8% vs 52.5%, χ2=4.342, df=1, p<.05), joint family (33.3% vs 7.5%, χ2=9.519, df=1, p<.05) and rural background (82.1% vs 62.5%, χ2=5.453, df=1, p<.05), and had poor control of the level of HbA1c (55.1% vs 2.5%, χ2=41.022, df=2, p<.01) than those without co-morbid depression. There was a significant negative correlation of years in education with HAM-D total score (r=-.471, p<0.01). Conclusion: A co-morbidity of depression is very common in patients of type-2 diabetes mellitus and various sociodemographic and clinical factors are linked to this comorbidity. It is important to address these issues for the sake of overall better outcome in type-2 diabetes mellitus.
Association of vitamin D levels with simple febrile seizures in under five children: a case control study
(Medip Academy, 2019-03) Singh, Virender; Sharma, Preeti; Dewan, Deepika
Background: There is increasing concern that vitamin D deficiency may play etiological role in Febrile seizures. Few studies have reported vitamin D deficiency in children with recurrent febrile seizures and hypocalcemia seizures. 'The objective of this study is to explore vitamin D status in children aged 6 months to 5 years with first episode of febrile seizure and to find the association between vitamin D levels and febrile seizure.Methods: A hospital-based case control study was conducted in sub district hospital, Katra over a period of three years. Cases were children of age group 6 months to 5 years presenting with first episode of simple febrile seizure to the casualty or OPD. For each case, a control was selected with similar age group and same sex who came for short duration fever (<2 days) but without seizures. A 5ml of blood sample for measurement of 25 hydroxy vitamin D was taken from the peripheral vein of each participant by trained laboratory technician. In order to categorise various degrees of vitamin D deficiency, Indian Academy of paediatrics criteria was used. Results were presented in the form of percentages and Odds ratio was calculated as measure of association.Results: A total of 75 cases and 75 controls were included in the study. Cases and controls were comparable as per baseline characteristics. Strong and significant (p<0.01) association of febrile seizures with vit D levels was observed.Conclusions: There was a high prevalence of vitamin D insufficiency among cases of simple febrile seizure.
Clinical Study of Maternal Near Miss
(International Research Organization for Life & Health Sciences (IROLHS), 2019-11) Gupta, Mayuri; Sharma, Preeti
Introduction: The women who survive serious complications of pregnancy are referred to as “near miss.” For every maternaldeath, there are many others who suffer serious life-threatening complications of pregnancy, referred to as “near miss” morbidity.In fact, for the over 500,000 mothers who die annually worldwide, and mostly in developing countries, there are more than 8million who suffer severe maternal morbidity (WHO 2004). There is currently no standard definition of “near miss” such as thereis for a maternal death because it is difficult to determine exactly at which point a woman becomes a “near miss.” WHO definesmaternal “near miss” as a woman who nearly died but survived complications that occurred during pregnancy, childbirth, orwithin 42 days of termination of pregnancy.Aims and Objectives: The aim of the study was to determine the magnitude and types of life-threatening maternal complicationsin pregnant and recently delivered women, timing and management, blood transfusion and major surgery, and inpatient durationof stay of patients with “near miss” morbidity.Materials and Methods: The prospective cohort study was done by the Department of Obstetrics and Gynaecology, KamlaRaja Hospital, Gwalior, study period from 1 year, November 2014 to October 2015. Inclusion Criteria: Acute cyanosis, Gasping,Respiratory rate >40 or <6/min, shock, oliguria, clotting failure, loss of consciousness lasting >12 h, stroke, uncontrolled fit/totalparalysis, and jaundice in the presence of pre-eclampsia were included in the study. Exclusion Criteria: Morbidity resulting fromcauses not related to pregnancy or its complication or management, for example, malignancies, ca breast, and liver rupture.Morbidity from accidental or incidental causes no way related to pregnancy, for example, morbidity from automobile accident/suicide. Women who develop these conditions unrelated to pregnancy.Results: Near miss to maternal death ratio as 3.69:1 which means out of five women with severe morbidity we are savingfour cases. Near miss cases are 97 (61%) war multigravida and only 61 (39%) war primigravida. Referred cases were 102to which means 66.1% of near miss were referred to our institute from various center. Only 57 cases (33.9%) came directly.Fifty-four patients (33.9%) have 4 days intensive care unit (ICU) stay followed by 50 cases (31.44%) having 3 days ICU stayand maximum stay was of 6 days in 11 cases.Conclusion: Still, it needs improvement, which can be achieved by ongoing training and simulation sessions for obstetricalstaff in early recognition and management of severe obstetric morbidity and also by resource allocation that is required in themanagement of the near miss cases.
Clinicopathological analysis of extracranial head and neck schwannoma: A case series
(Wolters Kluwer India Pvt. Ltd., 2019-05) Sharma, Preeti; Zaheer, Sufian; Goyal, Surbhi; Ahluwalia, Charanjeet; Goyal, Ankur; Bhuyan, Geet; Mandal, Ashish Kumar
Background: Extracranial head and neck schwannomas are rare tumors which are often clinically misdiagnosed. Preoperative diagnosis of these tumors is challenging but imperative for surgeons so as to avoid nerve damage during excision. Materials and Methods: Sixteen patients diagnosed with extracranial head and neck schwannomas over a period of 2 years were retrospectively analyzed. Clinical details including preoperative fine-needle aspiration cytology (FNAC) and/or computed tomography (CT)/magnetic resonance imaging (MRI) findings were retrieved. FNAC smears and formalin-fixed paraffin-embedded sections were evaluated. Results: Among these 16 tumors, 6 (37.5%) were located in the lateral aspect of neck, 4 (25%) in scalp, 2 (12.5%) in orbit, and one each (6.25%) in palate, tongue, submandibular gland and parotid gland. The mean patient age was 31.3 years. FNAC was performed in 14 cases, of which 8 cases (58.3%) showed features of benign nerve sheath tumor (BNST), two cases (14.2%) were inconclusive with possibility of mesenchymal lesion, two cases (14.2%) were inadequate, one case (8.3%) showed features suggestive of schwannoma, and a diagnosis of nerve sheath tumor inconclusive for malignancy was rendered in a single case. The sensitivity of FNAC in diagnosis of BNST was 71.4%. CT or MRI was performed in five cases, of which an accurate diagnosis was rendered only in one case of orbital schwannoma. Conclusion: Imaging has a limited role in the preoperative diagnosis of head and neck schwannomas owing to nonspecific radiological features. Cellular aspirate smears are helpful in accurate diagnosis even at unusual locations
Combined hysteroscopy and laparoscopy in evaluation of female infertility
(Medip Academy, 2019-01) Gandotra, Nikita; Rizvi, Syed Masuma; Sharma, Preeti
Background: Infertility has always been one of the most elusive symptom complexes that perplex the best gynecologists and affects about 10-15% of reproductive age couples.Methods: This descriptive study was conducted in the Postgraduate Department of Obstetrics and Gynaecology at Government Medical College, Jammu for a period of 18 months in which 100 infertile females attending OPD for infertility workup were subjected to detailed relevant history taking followed by physical examination. Diagnostic hysteroscopy and laparoscopy along with laparoscopic chromopertubation was carried out after written informed consent including minimal therapeutic interventions if required.Results: Out of 100 patients included in the study, 67 (67%) presented with primary infertility and 33 (33%) presented with secondary infertility. Combined laparoscopy and hysteroscopy was normal in 13% (13) of cases. Out of 100 patients, abnormal laparoscopic findings were noted in 73% of patients: Endometriosis ( 13%),Polycystic ovaries (12%), Unilateral tubal block (11%), Pelvic inflammatory disease (9%).Abnormal hysteroscopic findings were noted in 47% of patients: Submucous myoma (8%),unilateral ostial block (8%),Endometrial polyp (7%), Hyperplasic endometrium (7%), Cervical stenois (6%).Most common cause of infertility in present study on Combined hysteroscopy and laparoscopy was Endometriosis (13%) followed by Polycystic ovaries (12%),Uterine myoma (12%) out of which 5% myomas were diagnosed on laparoscopy and 8% on hysteroscopy and 1% had myoma diagnosed on both hysteroscopy and laparoscopy. In the primary infertility group most, common finding was Polycystic ovaries (16.4%) followed by Endometriosis (14.9%) and uterine myoma (14.9%) while in patients suffering from secondary infertility, most patients had Bilateral tubal block (18.2%), Pelvic inflammatory disease (12.1%), Unilateral tubal block (12.1%), intrauterine adhesions (12.1%).Conclusions: Combined Hysteroscopy and Laparoscopy is a quintessential tool that provides cost-effective, comprehensive and single set-up diagnostic aid in infertile patients.
Comparative efficacy of Solanum xanthocarpum extracts alone and in combination with a synthetic pyrethroid, cypermethrin, against malaria vector, Anopheles stephensi.
(2007-03-02) Mohan, Lalit; Sharma, Preeti; Srivastava, C N
With a goal of minimal application of environmentally hazardous chemical insecticides, the larvicidal activity of cypermethrin was studied alone and in combination with the root extract of Solanum xanthocarpum against anopheline larvae. Petroleum ether extract was observed to be the most toxic, with LC,, of 1.41 and 0.93 ppm and LC90 of 16.94 and 8.48 ppm at 24 and 48 hours after application, respectively, followed by carbon tetrachloride and methanol extracts. The values for cypermethrin were an LC50 of 0.0369 ppm after 24 hours and 0.0096 ppm after 48 hours and LC90 of 0.0142 and 0.0091 ppm after 24 and 48 hours, respectively. The ratios of cypermethrin and petroleum ether extracts tested were 1:1, 1:2 and 1:4. Of the various ratios tested, the cypermethrin and petroleum ether extract ratio of 1:1 was observed to be more efficient than the other combinations. From the individual efficacy of each constituent, synergism was noted. This is an ideal ecofriendly approach for the control of malaria vector, Anopheles stephensi.
Comparative reliability of cheiloscopy and palatoscopy in human identification.
(2009-10) Sharma, Preeti; Saxena, Susmita; Rathod, Vanita
Background: Establishing a person's identity in postmortem scenarios can be a very difficult process. Dental records, fingerprint and DNA comparisons are probably the most common techniques used in this context, allowing fast and reliable identification processes. However, under certain circumstances they cannot always be used; sometimes it is necessary to apply different and less known techniques. In forensic identification, lip prints and palatal rugae patterns can lead us to important information and help in a person's identification. This study aims to ascertain the use of lip prints and palatal rugae pattern in identification and sex differentiation. Materials and Methods: A total of 100 subjects, 50 males and 50 females were selected from among the students of Subharti Dental College, Meerut. The materials used to record lip prints were lipstick, bond paper, cellophane tape, a brush for applying the lipstick, and a magnifying lens. To study palatal rugae, alginate impressions were taken and the dental casts analyzed for their various patterns. Results: Statistical analysis (applying Z-test for proportion) showed significant difference for type I, I', IV and V lip patterns (P < 0.05) in males and females, while no significant difference was observed for the same in the palatal rugae patterns (P > 0.05). Conclusion: This study not only showed that palatal rugae and lip prints are unique to an individual, but also that lip prints is more reliable for recognition of the sex of an individual.
Correlation of admission labour admission test in low risk pregnancies with pregnancy outcome
(Medip Academy, 2019-05) Sharma, Preeti; Gandotra, Nikita; Rana, Deepti; Rasheed, Sabia; Sharma, Anil Kumar
Background: Aim of the study was to evaluate the role of NST (labour admission test) as a screening method in management of low risk pregnancies and to study the correlation of NST with fetal outcome.Methods: A prospective observational study conducted over 500 patients managed at our centre after proper evaluation. Patients were evaluated for mode of delivery and neonatal outcome.Results: The maximum number 352 of patients belonged to 20-30 years age group, 113 patients belonged to 31-35 age group. 200 patients were of 37-40 weeks gestation and 185 were of 40-41 weeks gestation. There were 125 patients in the 41-42 weeks gestational period. Among the 500 pregnant mothers who were included in the study 82.6% had Normal NST, 11.6% had suspicious and 5.8% had pathological NST. Cesarean rate was 14.4% in normal NST group, 58.62% in suspicious group and 72.41% in pathological group study. 6.77% in Normal group had meconium stained liquor at delivery whereas 29.31% in suspicious group and 37.93% in pathological group had same.Conclusions: The non-stress test is an important screening tool to identity the fetus in jeopardy in utero. This enables an appropriate timely intervention to achieve the most favorable outcome.
A cross-sectional study to assess the knowledge, attitudes, and practices of cervical cancer screening/pap smear among health care professionals in a tertiary care hospital in North India
(Medip Academy, 2023-12) Aggarwal, Richa; Mathe, Priyanka; Sharma, Preeti
Background: Cervical cancer is one of the most easily preventable and curable cancers among females. It imposes a high burden on developing countries, and with the use of screening methods, higher detection rates can be assured. Aims and objectives were to evaluate the knowledge, attitudes, and practices (KAPs) among health care professionals including doctors, nurses, and laboratory technicians regarding a very simple test for cervical cancer screening (i.e., Pap smear)Methods: This was a descriptive cross-sectional study that was conducted on 200 female health care workers in University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi from August 2018 to July 2022. A pretested, self-administered questionnaire was used on health care professionals working in various departments. This questionnaire collected information on sociodemographic data, knowledge regarding cervical cancer screening, Pap smear, and HPV vaccination, and attitudes of participants toward a Pap smear and HPV vaccination.Results: A total of 200 health care workers participated and knowledge among doctors and paramedical staff was compared but only 24.05% of the doctors and 26.44% of the paramedical staff had ever undergone a Pap smear screening, which was statistically insignificant (p=0.7). Awareness about the cervical cancer prevention vaccine was found more among doctors (n=78, 98.7%) than paramedical staff (n=77, 63.63%), and this was found to be statistically significant (p=0.001) Also, data among participants from a gynecology department (GD) versus other departments was compared, and awareness regarding HPV vaccine was much higher in the other-than-gynecology department (OTGD) group (84.29%) versus the GD group (59.54%), and this was statistically significant (p<0.001).Conclusions: Health care workers play an essential role in the community to spread awareness, but there is a need for a change in attitude about the disease and its screening. We can utilize this knowledge in filling the critical gaps in the community regarding awareness, screening, and prevention of the disease.
Current and futuristic scope of biomarkers in acute myocardial infarction
(Scientific Scholar, 2018-04) Verma, Manish Kumar; Sharma, Preeti; Kumar, Pradeep; Singh, Anand Narayan
Introduction: Acute myocardial infarction is a leading cause of death throughout the world. Myocardial infarction is defined as myocardial cell death due to prolonged ischemia. Objectives of the study were to look for the role of inflammatory markers (CRP, IL-6, and plasma fibrinogen) in the prediction of myocardial infarction. In recent times, IL-6 and plasma fibrinogen are not established marker in acute myocardial infarction. However, there is another biomarker i.e. CRP that have shown additional value in improving sensitivity and prognostic information. Novel biomarkers have improved assessment of outcome in acute myocardial infarction, but none have been demonstrated to alter the outcome of a particular therapy or management strategy. Thus the finding of this study may help the clinician to develop more novel therapeutic strategies for the management of myocardial infarction (MI) patients. The outcome of the study will be very beneficial as well, to the researcher working in the concerned area in order to develop more focused research approach
Diagnostic dilemma of antineutrophil cytoplasmic antibody seropositivity in human immunodeficiency virus infection.
(2011-01) Mohapatra, Prasanta R; Khanduri, Sushant; Dutt, Naveen; Sharma, Preeti; Janmeja, Ashok K
We present a case of a 48-year-old male who was diagnosed and treated for Wegener’s granulomatosis on the basis of history, clinical features, computed tomography (CT) and antineutrophil cytoplasmic antibodies (ANCA) positivity. The patient initially improved and later on during course of the disease he was found to be human immunodeficiency virus (HIV) seropositive. The potential pitfalls of cANCA in a HIV-infected patient are discussed.
Dissesiminated cysticercosis with neurocysticercosis – A rare presentation diagnosed by fine needle aspiration cytology
(Medip Academy, 2019-09) Singhal, Shipra; Shushmita, Shweta; Sharma, Preeti
Disseminated cysticercosis is a rare presentation of human T. Solium infection in which the parasite disseminates via the blood stream throughout the human body. The various clinical manifestations depend upon the location of the parasitic cyst inside the body. Neurocysticercosis is the most common parasite disease of the central nervous system. Disseminated cysticercosis with neurocysticercosis is a very rare presentation of human cysticercus infection. Here we present such a rare case in which a young man presented with multiple swellings all over the body and a history of seizures. Fine needle aspiration cytology was done and the diagnosis was established.
Epithelial–mesenchymal transition in serous and mucinous epithelial tumors of the ovary
(Wolters Kluwer India Pvt. Ltd., 2019-12) Bhuyan, Geet; Arora, Rashmi; Ahluwalia, Charanjeet; Sharma, Preeti
Context: The concept of epithelial–mesenchymal transition (EMT) in cancer origin, progression, and metastasis is of recent origin and not fully understood. So far, many cell culture studies have been done to investigate the role of EMT in epithelial ovarian cancer, but only a few human studies have been conducted. Aims: The aim of the study is to study the expression of E-cadherin and vimentin in serous and mucinous tumors of the ovary and to compare their expression in benign and malignant serous and mucinous ovarian tumors. Methods: This study was a prospective study done on 60 patients with a histological diagnosis of serous and mucinous ovarian malignancy. The study was conducted in the Department of Pathology and Department of Obstetrics and Gynaecology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi. The expression of epithelial marker E-cadherin and mesenchymal marker vimentin was studied in each of the tumors. Statistical Analysis: Unpaired t-test/Mann–Whitney test, Chi-Square test/Fisher's exact test, and Kolmogorov–Smirnov test were used. Results: Of the total 60 cases included in the study, 30 benign and 30 malignant cases of serous and mucinous tumors were taken. Of the 30 benign cases, 22 cases (73.3%) were that of serous cystadenomas, whereas 8 (26.67%) cases were of mucinous cystadenomas. Among the malignant cases, 21 cases (70%) were serous surface epithelial ovarian carcinoma, whereas 9 (30%) were mucinous surface epithelial ovarian carcinoma. Subsequently, the malignant cases were graded according to their glandular differentiation. Immunohistochemistry was performed in each of the 60 cases. Conclusion: In the malignant cases with increasing grade of the tumor, a reduced expression of E-cadherin and an increased expression of vimentin were seen in the epithelial cells
Evaluation of FNAC in Bone Lesions
(Society for Health Care & Research Development, 2019-01) Mohit; Dixit, Sachi; Sharma, Rachna; Sharma, Preeti; Kumar, Pradeep
The aim of the present study is to evaluate the utility of FNAC in diagnosing bone lesions especially bone tumours. Methods: A total of 120 cases were selected from patients attending OPD and admitted in IPD in MLB Medical College, Jhansi, presenting with complaints of swelling arising from bone. FNAC of the lesion was performed, with guidance of X-Ray reports when available, clinical details, history of present illness & other physical findings have also been noted, and considered while making the diagnosis, and the findings were compared with that of histolgical specimen whenever available. Results: The present study includes 355 malignant cases, of which 53.8% are primary neoplasms, 7.6% are metastatic lesions, and 38.4% are locally malignant lesions. The most common primary bone malignancy in this study is Osteosarcoma. Comparison of Cytological and Histological Diagnosis in all cases was done. 39 malignant lesions were reported on Cytological basis and all 39 were found correct on histological correlation, while 18 out of total 21 benign lesions were diagnosed correctly, as confirmed by subsequent histological examination. Out of total 51 inflammatory lesions 42 were diagnosed correctly, 9 aspirations were found inadequate for reporting. Conclusion: Fine Needle Aspiration Cytology (FNAC) is a very valuable procedure in patients with bone lesions. The complications are almost nil, the cost is very low, is minimally painfull, is an OPD procedure and informative reports are available within 12-48 hours.
Evaluation of Mast Cell Profile in the Skin Lesions of Leprosy
(Society for Health Care & Research Development, 2018-11) Mohit; Dixit, Sachi; Sharma, Rachna; Sharma, Preeti; Kumar, Pradeep
Background: Aim of the present study was to evaluate the role of Mast cells in the Histo-Pathogenesis of Leprosy by assessing incidence of various Histo-Pathological types of Leprosy, Correlating of Mast cell profile (Mast cell Density & Morphology) in different Histological types of Leprosy lesions, by correlating with Reactional & Non-Reactional groups of Leprosy cases and by correlation of Mast cell profile in doubtful cases as in Indeterminate type. Lepromatous Leprosy was observed in higher age group as compared to Tuberculoid leprosy. Mast Cell Density was found increasing from Polar Tuberculoid to Polar Lepromatous spectrum of disease. In every type of Leprosy, mast Cell Density was reduced significantly in the Reactional Group as compared to Non-Reactional Group. The aim of the present study was to evaluate the role of Mast cells in the Histo-Pathogenesis of Leprosy by assessing incidence of various Histo-Pathological types of Leprosy. Methods: A cross sectional, observational study was conducted among 200.Correlation of mast cell profile (Mast cell Density & Morphology) in different Histological types of Leprosy lesions was done, by correlating with Reactional & Non-Reactional groups of Leprosy cases and by correlation of Mast cell profile in doubtful cases as in Indeterminate type. Lepromatous Leprosy was observed in higher age group as compared to Tuberculoid leprosy. Results: Mast Cell Density was found Increasing from Polar Tuberculoid to Polar Lepromatous spectrum of disease. In every type of Leprosy, mast Cell Density was reduced significantly in the Reactional Group as compared to Non-Reactional Group. Conclusion: Larger such studies of Leprosy employing special staining methods viz. pH dependent Toluidine Blue staining, studies employing Immuno-Fluorescence on Tissue biopsy specimens, as well as studies on experimental animals, to help us achieve a better understanding of the pathogenesis, outcome and prognosis of Leprosy.
Evaluation of Solanum xanthocarpum extracts as mosquito larvicides.
(2005-06-13) Mohan, Lalit; Sharma, Preeti; Srivastava, C N
Mosquito larvicidal activity of crude carbon-tetra-chloride, methanol and petroleum ether extracts of Solanum xanthocarpum fruits was examined against Anopheles stephensi and Culex quinquefasciatus. Among the extracts tested, carbon-tetra-chloride extract was the most effective with LC50 values of 5.11 ppm after 24 hours and 1.27 ppm after 48 hours of treatment against An. stephensi. In the case of Cx. quinquefasciatus the petroleum ether extract was observed as most toxic with LC50 values of 62.62 ppm after 24 hours and 59.45 ppm after 48 hours of exposure period respectively. It is, therefore, suggested that S. xanthocarpum can be applied as an ideal potential larvicide against An. stephensi and Cx. quinquefasciatus.
Genital tuberculosis in infertile women: role of hysterolaparoscopy and tuberculosis polymerase chain reaction
(Medip Academy, 2019-07) Gandotra, Nikita; Sharma, Abhinav; Sharma, Preeti
Background: Genital tuberculosis is an important cause of female infertility in developing countries like India. It is one of the major causes for severe tubal disease leading to infertility.Methods: A prospective study was conducted in which 100 women presented to hospital with infertility were subjected to hystero-laparoscopy over 1 year. Endometrium sent for tuberculosis polymerase chain reaction (TB-PCR) and HPE and results were formulated.Results: Out of 100 women, 28% were diagnosed with Genital tuberculosis (GTB) using accepted clinical criteria, TB-PCR and endometrial HPE. 25 of these 28 were diagnosed by hysterolaparoscopy (89.24%) alone, 16 by positive endometrial TB-PCR (57.14%) and another 2 by HPE (7.14%).Conclusions: In country like ours where TB is endemic, a multi-pronged approach to diagnosis increases the chances of successfully diagnosing this destructive disease.
Grading angiogenesis in oral squamous cell carcinoma: A histomorphometric study.
(2015-01) Wadhwan, Vijay; Sharma, Preeti; Saxena, Chitrapriya; Venkatesh, Arvind
Context: Like normal tissues, tumors require an adequate supply of oxygen, metabolites and an effective way to remove waste products. This is achieved by angiogenesis, which is defined as the process by which new blood vessels are produced by sprouting from preexisting vasculature. There is a large spectrum of physiological and pathological processes in which angiogenesis occur, ranging from tissue hypertrophy, wound healing, and inflammation to tumors. Aims: The present study was designed to morphometrically evaluate the angiogenesis in different grades of oral squamous cell carcinomas (OSCCs) under light microscope by the use of H and E stained sections and to assess that whether the parameters of vascularity like mean vascular density (MVD), mean vascular area (MVA), and total vascular area (TVA) can be used to histologically grade the tumors. Subjects and Methods: A total of 10 cases each of well‑, moderately‑ and poorly‑differentiated SCC cases were retrieved from the archives of the Department of Oral Pathology and Microbiology and were morphometrically analyzed for mean vascular density (MVD), MVA, and TVA. Ten cases of normal oral mucosa were taken as Control. Statistical analysis was done using SPSS 19.0 version (IBM, Armonk, NY, USA) software for windows. Group mean for MVD, TVA and MVA were calculated for 10 cases of each group. “Student’s t‑test” was applied to assess the intergroup variation of mean values of MVD, TVA, and MVA. Results: Our results showed significant differences between all the three parameters, that is, MVD, MVA and TVA when poorly differentiated OSCC was compared with the normal mucosa, well‑ and moderately‑differentiated OSCC. However, when comparison was made between the well‑ and moderately‑differentiated OSCC, the differences in the three parameters were present but not statistically significant. Conclusion: There was an increased MVD, MVA and TVA in poorly differentiated OSCC, which could be used as an additional criterion to histologically grade the tumor.
Hodgkin's lymphoma arising in a case of mycosis fungoides: An unusual association
(Indian Association of Dermatologists, Venereologists & Leprologists, 2018-05) Sharma, Preeti; Goyal, Surbhi; Yadav, Amit Kumar; Singh, Jasmeet; Mandal, Ashish Kumar
Mycosis fungoides is a cutaneous T-cell lymphoma with a high risk for developing secondary malignancies, especially B-cell lymphoproliferative disorders. About 40 cases of Hodgkin's lymphoma associated with mycosis fungoides have been reported in literature till date. We report a case of a 35-year-old gentleman who presented with intensely itchy reddish lesions all over the body. Multiple skin biopsies taken from the lesions on scalp and back confirmed the clinical diagnosis of mycosis fungoides. While on treatment, he presented with multiple bilateral cervical, axillary and inguinal lymphadenopathy 9 years after the primary diagnosis of mycosis fungoides. Excision biopsy of a cervical lymph node revealed partial effacement of architecture by a tumor comprising polymorphous background. Histopathology and immunohistochemistry revealed a diagnosis of Hodgkin's lymphoma - nodular sclerosis subtype. The patient was started on chemotherapy for stage IV Hodgkin's lymphoma. Our case emphasizes the importance of keeping secondary Hodgkin's lymphoma in mind while dealing with a patient of mycosis fungoides. Our case immunohistochemically supports the distinct etiopathogenesis of Epstein–Barr virus-negative Hodgkin's lymphoma vis-à-vis cutaneous mycosis fungoides.

Browsing by Author "Sharma, Preeti"

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