Browsing by Author "Seshadri, M"
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Item Adoptive immunotherapy of murine lymphosarcoma: comparative study using allo-immune or tumor-immune T cells.(1997-06-01) Thakur, V S; Seshadri, M; Shah, D H; Poduval, T BThe antitumor effect of allosensitization with lymphocytes and skin graft of DBA/2 mice was evaluated using immunogeneic, transplantable Lymphosarcoma (LS-A) syngeneic to Swiss mice. A dose dependent tumor inhibitory effect in terms of tumor free mice was observed in mice sensitized i.p. with lymphocyte doses between 10-100 million per animal. Sensitization with allogeneic primary skin graft was more effective than lymphocyte immunization. The antitumor immunity could be adoptively transferred in syngeneic Swiss mice using either allo-immune or tumor-immune T cells. Analysis of T cell phenotypes using monoclonal antibodies against cell surface markers CD4 and CD8, indicated absolute dependence on the CD4+ T cells subset in tumor cure in case of allo-immune as well as tumor-immune T cells. CD8+ T cell subset was found essential only in case of allo-immune T cell therapy. Immunosuppression of mice with whole body gamma irradiation (4 Gy), 6 hr before transfer of allo-immune or tumor-immune T cells did not abrogate the therapeutic ability of allo-immune or tumor-immune T cells. Our results suggest that allosensitization could be an effective method of generating effector lymphocyte populations that might be used to treat tumors that exhibit detectable immunogenecity.Item Allosensitization induced suppression of various murine tumors: role of non-H-2 antigens in antitumor immunity.(1990-08-01) Thakur, V S; Seshadri, M; Poduval, T B; Shah, D H; Sundaram, KPresence of alloantigens on various murine tumors was tested by tumor rejection in allosensitized Swiss mice. The results indicated the presence of alloantigen on immunogenic tumors like chemically induced fibrosarcoma (FS), ascitic sarcoma 180 (S 180) and immunogenic variant of lymphosarcoma (LS-A) in Swiss mice, while these antigens could not be detected by this procedure on spontaneous lymphosarcoma (LS). Allosensitization with skin graft was found to offer quantitatively higher antitumor resistance than the allosensitization achieved by allogeneic lymphocytes. Antitumor effect was not seen when tumor cells were inoculated earlier than day 3 of grafting. Further, host immunosuppression with whole body irradiation up to day of 3 of skin grafting abrogated the antitumor effect. H-2 compatible and non-H-2 incompatible skin graft sensitization of host could offer resistance against both S 180 and LS-A. Further, tumor immune mice rejected H-2 compatible, non-H-2 incompatible skin graft significantly earlier.Item Analysis Of Y-Linked Lineage Markers In Indian Populations.(1998-01) Das, Birajalaxmi; Seshadri, M; Chauhan, P SRecently, Y-linked markers have been found extremely useful tools in population genetics and evolutionary biology. We have studied a tetranucleotide marker, DYS19 and a bialleleic marker DYS287, an Alu polymorphic marker (YAP) in five ethnic groups of India. Blood samples were collected from 215 random healthy males and DNA was extracted by using a simple salt precipitation method. DNA samples, after amplification by polymerase chain reaction (PCR), were electrophoresed. The detection of alleles was done by silver staining and reconfirmed by using a fragment analyser programme in an ALF express sequencer (Pharamacia). A total of four alleles were observed at DYS19 locus in all the five ethnic groups. Allele 194bp was found to be the predominant one in all the groups. The absence of YAP was found in all the groups.Item Cell cycle dependent binding of radiolabelled concanavalin A by amoeba.(1979-08-01) Chatterjee, S; Seshadri, M; Ray, P KItem Comparative study of repetitive DNA in Phaseolus.(1981-08-01) Seshadri, M; Ranjekar, P KItem D17S5 Minisatellite In Two Maharastrian Indian Populations : Overcoming The Differential Amplification At This Locus Using Expand Long Template PCR System.(1998-01) Ghosh, Anu; Seshadri, M; Chauhan, P SThe D17S5 is a highly informative minisatellite locus and shows extensive interpopulation allelic variability. However in many cases, it shows preferential amplification of shorter alleles, which has hampered the full potential of this minisatellite from being realized. This study explores the use of the Expand Long Template PCR system to circumvent this problem.Item D1S80 Minisatellite - Polymorphism In Five Ethnic Populations Of Maharashtra And Kerala.(1998-01) Das, Birajalaxmi; Seshadri, M; Chauhan, P SThe minisatellite locus D1S80 was studied in five ethnic populations, namely, Konkanastha Brahmins and Marathas of Maharashtra; Nairs, Ezhavas and Muslims from Kerala, to compare the allele and genotype frequencies. DNA isoleted from blood samples collected from 364 unrelated healthy donors was amplified by PCR and subjected to polyaerylamide gel electrophones. A total of 20 alleles and 71 genotypes were observed from the above population groups. A bimodal pattern was observed in the allele frequency distribution representing 18 and 24 as predominant ones. The observed heterozygosity was in the range of 65% to 82%. All the five population groups conformed to Hardy- Weinberg equilibrium expectations.Item Effect of host sensitization to tumour on splenic depletion & recovery following severe immunosuppressive treatment.(1979-10-01) Poduval, T B; Seshadri, M; Ray, P K; Thakur, V S; Sundaram, KItem Effect of immunization of mice with syngeneic embryos on their ability to inhibit the growth of a challenging fibrosarcoma.(1980-09-01) Ray, P K; Seshadri, MItem Effect of multigeneration alcohol feeding on murine immune system.(1990-09-01) Poduval, T B; Seshadri, M; Thakur, V S; Chauhan, P SMice belonging to F8, F12, F14 and F20 generation of a multigeneration study reared on 20% (v/v) ethanol in water as the sole drinking source were investigated for their immune competence using various parameters. The results indicated lack of any significant effect on delayed type hypersensitivity to dinitro fluorobenzene (DNFB) or sheep red blood cells (SRBC) in mice consuming ethanol. Further, alloskin graft and tumor graft response was similar in both ethanol and water fed mice. Humoral response to SRBC was also intact. However, NK cell activity was reduced significantly in ethanol fed mice. Phagocytic index as assessed by the carbon clearance test was also reduced considerably in mice consuming ethanol. The results clearly indicate that ethanol per se has a significant effect on the nonspecific limb of the immune system, in chronically fed mice.Item Genome characterization of three plant species belonging to the genus Phaseolus.(1979-02-01) Seshadri, M; Ranjekar, P KItem Influence of parity status of female mice on growth of a transplantable chemically induced fibrosarcoma.(1981-05-01) Ray, P K; Seshadri, MItem Influence of pregnancy & fibrosarcoma on hepatic mitochondria of mice.(1977-06-01) Tarachand, U; Seshadri, M; Eapen, JItem Inhibition of growth of rat Yoshida sarcoma using a neuraminidase treated tumour vaccine.(1979-01-01) Ray, P K; Seshadri, MItem Studies On ApoB VNTR In Two Maharashtrian Ethnic population Groups.(1998-01) Das, Birajalaxmi; Seshadri, M; Chauhan, P SThe ApoB VNTR locus was analyzed in two distinct ethnic groups of Maharashtra to determine, the distribution of allele and genotype frequencies. Blood samples were collected from 183 random, unrelated, healthy donors. DNA was extracted by using a simple salt precipitation method, amplified by polymerase chain reaction (PCR) and the products were electrophoresed in 4% PAGE followed by silver staining. A total of 14 alleles and 38 genotypes were observed. Allele 37 and 39 were found to be the predominant alleles showing a bimodal distribution. Both the population groups conformed to Hardy-Weinberg Equilibrium expectations.Item Successful management of Bland--White-Garland syndrome in infancy.(1992-03-01) Reddy, K N; Ranjit, S; Jagannath, B R; Seshadri, M; Balakrishnan, K R