Browsing by Author "Sakly, Mohsen"
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Item Antioxidant activity of extract of Rhus oxyacantha root cortex.(2006-03-17) Tebourbi, Olfa; Trabelsi, Chiraz; Ben Nasr, Cherif; Sakly, MohsenIn the present study, the root extract of Rhus oxyacantha contained 25.33 mg of catechin equivalent per mg of fresh wt and was found rich in proanthocyanidins compared to vine shoot, grape pips and leaves. The chromatographic analysis of the extract suggested the presence of (+) catechin, (-) epicatechin -3-O-gallate as well as proanthocyanidinic oligomers and polymers. Root cortex inhibited the ascorbic acid oxidation by dioxygen. It also prevented DDT-induced thymocytes death in a dose-dependent manner. The results suggested antioxidant property of root extract of Rhus oxyacantha which could be ascribed to its free radical scavenging nature.Item Dieldrin initiates apoptosis in rat thymocytes.(2002-10-16) Hallegue, Dorsaf; Ben Rhouma, Khemais; Krichah, Raja; Sakly, MohsenIn vitro incubation for 6 hr to pesticide dieldrin resulted in a dose-dependent decrease of cell viability comparable to that of dexamethasone. In vivo experiments also demonstrated that dieldrin administration induced a dose-dependent thymic atrophy which appeared to be mediated by endogenous corticosteroids. Agarose gel electrophorosis analysis, revealed the generation of typical apoptotic oligosomal DNA fragmentation in presence of dieldrin. However, in response to high concentrations of pesticide, cells seemed to undergo necrosis pathway. Thus, it may be concluded that dieldrin induced apoptosis in rat thymocytes.