Browsing by Author "Rath, S."

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    Evaluation of the antibacterial activity of Coccinia grandis, against bacteria isolated from chronic suppurative otitis media infection
    (Open Science Publishers LLP, 2023-01) Dubey, D; Swain, SK; Lenka, S; Meher, RK; Kar, B; Rath, S.
    This present study evaluates the in vitro antimicrobial activity of the selected Indian medicinal plant Coccinia grandis (C. grandis) against this isolated multidrug-resistant bacteria causing chronic suppurative otitis media infection (CSOM) along with their in vivo toxicity test using a rat model. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of the effective leaf extract were measured using the micro broth dilution method. Out of 128 samples, 79 samples were identified as positive for bacterial infection. Out of six solvent extracts of C. grandis methanol and ethyl acetate, leaf extracts have shown the best antimicrobial activities compared to the rest of the four solvent extracts. Methanolic extract 1.56 mg/ml and 0.78 mg/ml was the MIC values for the respective plants. Staphylococcus aureus and Pseudomonas aeruginosa species are the most common bacterial isolates in CSOM infection. Hence, the causative organism and its drug sensitivity pattern should be carried out before treatment. Due to increasing cases of antibiotic resistance among bacteria, an alternative therapy is the need of the day, especially regarding the needier population with lesser toxicity. Hence, plants can be used as a choice of drugs to treat CSOM infection.
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    Neuroprotective Effect Of Methanolic Extract Of Sargassum Wightii On Haloperidol Induced Catalepsy And Tardive Dyskinesia In Albino Rats
    (Innovare Academic Sciences Pvt. Ltd., 2020-03) Rout, S.; Rath, B.; Bhattamisra, S. K.; Kumar, A.; Rath, S.
    Objective: The present study was designed to evaluate the neuroprotective effect of methanolic extract of Sargassum wightii on haloperidol-induced catalepsy and tardive dyskinesia in Wistar albino rats. Methods: In this study, thirty Wistar albino rats were randomly divided into six groups. Gr-I served as control. Haloperidol (1 mg/kg intraperitoneally) was administered to rats of Gr-II to Gr-V for twenty-one consecutive days to induce catalepsy and tardive dyskinesia. Animals of Gr-II to Gr-V were orally administered with vehicle, levodopa carbidopa combination (30 mg/kg), Sargassum extract 200 and 400 mg/kg respectively. All the drugs and vehicles were given orally one hour before haloperidol injection for twenty one consecutive days. The cataleptic scores were recorded using standard bar test. Tardive dyskinesia was assessed in terms of vacuous chewing movement (VCM) and tongue protrusion (TP) scores. After behavioural testing, all animals were sacrificed on twenty-second day and various biochemical parameters like MDA, SOD and GSH were estimated in brain tissue. Results: Chronic administration of haloperidol significantly increased cataleptic scores, VCM and TP scores. (p<0.001) Sargassum wightii extract (400 mg/kg) significantly inhibited haloperidol-induced catalepsy, VCM and TP (p<0.001) Haloperidol increased MDA and decreased SOD and GSH in brain tissue to a highly significant extent (p<0.001) Sargassum extract at 400 mg/kg also significantly reversed the haloperidol-induced alteration in brain oxidative stress markers. Conclusion: Sargassum wightii inhibits haloperidol-induced catalepsy and tardive dyskinesia. Thus it may be used as a unique therapeutic adjunct for the prevention of neuroleptic-induced extrapyramidal symptoms, however, it has to be explored more.