Browsing by Author "Pravin, R"

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    Anticancer potential of L-asparaginase impregnated selenium-cyclodextrin nanobiocomposite against mouse B-cell lymphoma cells
    (The India Publications and Information Directorate (CSIR-NISCAIR), 2025-06) Baskar, G; Jessica, A; Yuvassri, R; Doris, B Vanessa; Aneesah, LA; Pravin, R
    Lymphoma remains a significant challenge in cancer treatment due to its high prevalence and resistance to conventional therapies. There is a growing demand for innovative and targeted treatment strategies that can enhance therapeutic efficacy while minimizing side effects. The present work focuses on the synthesis of selenium-cyclodextrin (Se-CyD) nanobiocomposite of L-asparaginase using the co-precipitation method for drug delivery against lymphoma cells. The size of the synthesized Se-CyD nanobiocomposite was found to be 29.74 nm. Using a scanning electron microscope, the spherical shape of the nanobiocomposite was observed. The absorption of the nanocomposite was observed in the range of 200 to 600 nm using a double-beam UV-Vis spectrophotometer. The FTIR spectrum showed peaks of transmittance at specific wavenumbers, indicating regions of low and high absorption due to the involvement of functional groups in the nanobiocomposite. The X-ray diffraction analysis revealed the crystalline structure of the nanocomposite by representing sharp and prominent peaks. The Methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay on CH27 mouse B-cell lymphoma cell line loaded with Se-CyD nanobiocomposite showed 65.9% toxicity at a concentration of 100 ?g/mL. This concentration represents the IC50 value of the Se-CyD nanobiocomposite for the CH27 mouse B-cell lymphoma cell line. Thus, a combination of selenium and cyclodextrin nanocomposite coated with L-asparaginase proves to have anticancer properties.
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    Synthesis of phycoerythrin-Ag-ZnO nanobiocomposite from marine red algae Porphyridium purpureum for anticancer applications against MCF-7 cell line
    (The India Publications and Information Directorate (CSIR-NISCAIR), 2024-06) Baskar, G; Keerthana, K; Supriya, A; Pravin, R; Abinesh, AR; Yuvaraaj, SA
    The focus on utilization of marine macroalgae for green synthesis of bimetallic nanoparticles with potential applications in cancer treatment has gained a lot of attention in recent years. In this present study, we synthesized a nanobiocomposite using the red pigment R-phycoerythrin from the marine red algae Porphyridium purpureum and explored its anticancer potential. This vibrant red fluorescent pigment plays a crucial role as a reducing and stabilizing agent. Ag-ZnO nanobiocomposite was synthesized by green approach using phycoerythrin as a capping agent. The synthesized nanobiocomposite was characterized using UV-Vis spectroscopy, XRD, FT-IR and SEM-EDX techniques. The obtained UV-Vis graph has confirmed the presence of Ag, Zn and phycoerythrin in the synthesized nanobiocomposite. The FT-IR showed the occurrence of Zn–O vibration peak along with hydroxyl and carboxyl groups. The XRD results confirmed the crystalline nature and hexagonal shape of the nanobiocomposite. The overall effect of the synthesized phycoerythrin-Ag-ZnO nanobiocomposite was studied by MTT assay to check its anticancer applications. From the obtained results, the IC50value was found to be 100 ?g against MCF-7 cell line. This confirms that the synthesized phycoerythrin-Ag-ZnO nanobiocomposite inhibited the growth of MCF-7 cell line and thus can be efficiently used as a photosensitive drug for chemotherapy in future.