Browsing by Author "Prasad, G B"
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Item Antigenic analysis of excretory-secretory products of Wuchereria bancrofti and Brugia malayi infective larval forms by SDS-PAGE.(1989-08-01) Kharat, I; Cheirmaraj, K; Prasad, G B; Harinath, B CExcretory-secretory (ES) products of W. bancrofti and the closely related B. malayi infective larval forms were analysed for their antigenic activity by SDS-PAGE followed by Western blotting as well as by gel elution-sandwich ELISA using filarial serum immunoglobulin-G (FSIgG) as a capture antibody. In W. bancrofti infective larval ES products, the protein molecules of 66, 46, 35, 33, 30 and 14 kDa molecular wt. showed antigenic activity by immuno blotting technique. In sandwich ELISA technique eventhough all SDS-PAGE fractions except ESA 6 (55-47 kDa) showed antigenic positivity, the fractions ESA 8 (37-31 kDa) and ESA 9 (31-25 kDa) showed high reciprocal antigen titre of 262144 and 32768 respectively. In B. malayi infective larval ES products, the protein molecules of 109, 102, 97 and 77 kDa molecular wt. showed reactivity with FSIgG by blotting technique, where as in sandwich ELISA except ESA 7 (47-37kDa), all fractions showed antigenic positivity. However, these fractions failed to show high antigen titre similar to W. bancrofti ES products with FSIgG.Item Detection and isolation of filarial antigen from hydrocoele fluid and its use in diagnosis.(1985-04-01) Malhotra, A; Prasad, G B; Harinath, B CItem Detection of filarial antigen in immune complexes in bancroftian filariasis by ELISA.(1983-12-01) Prasad, G B; Reddy, M V; Harinath, B CItem Disc/stick ELISA for diagnosis of bancroftian filariasis.(1986-07-01) Parkhe, K A; Prasad, G B; Das, A; Harinath, B C; Roebber, M; Hamilton, R GItem Efficacy of two different DEC regimens in the treatment of human filarial infection.(1990-03-01) Prasad, G B; Ramaprasad, P; Rao, V S; Bharati, M S; Harinath, B CTwo regimens of diethyl carbamazine (DEC) viz., 14 day and 5 day, were compared for microfilaricidal effect and side effects, in the treatment of bancroftian filariasis. The rate of successful treatment, cure rate and decrease in mf count were found to be significantly high with the 14 days regimen when assessed immediately after treatment. About 40 per cent of subjects on the 14 days regimen and 66 per cent of patients on 5 days regimen experienced side reactions. The severity of side reactions was more in patients on 5 days regimen. When the effect of DEC was assessed one year after treatment with the 14 days regimen and compared with the results immediately after treatment, the rate of successful treatment, cure rate and decrease in mf count were reduced significantly. The 14 days DEC regimen with initial low dose of DEC along with antipyretics may be better accepted in the control programmes of filariasis.Item Host preference of Culex quinquefasciatus in Raipur city of Chattisgarh state.(2001-03-20) Dixit, V; Gupta, A K; Kataria, O M; Prasad, G BIndoor resting freshly engorged Culex quinquefasciatus collected from three different habitats viz., human, cattle and mixed dwellings of six localities of Raipur city, were analyzed for the source of blood meal by precipitin test. Of the 60 specimens from human dwellings 52 were positive for human blood with an anthropophilic index of 90%. Of the 25 specimens from cattlesheds 15 were positive for human blood with an index of 60%. Of the 20 specimens from mixed dwellings 12 showed human blood with an anthropophilic index of 63.15%. Thus, the Culex quinquefasciatus at Raipur city in Madhya Pradesh state is predominantly anthropophilic in nature irrespective of the nature of habitat of the mosquito vector.Item Immune complexes & immunoglobulins involved in human filariasis.(1983-06-01) Prasad, G B; Reddy, M V; Harinath, B CItem Immunosuppression in clinical filariasis.(1987-05-01) Prasad, G B; Harinath, B CItem Isolation and evaluation of antigens from microfilaraemia plasma and immune complexes for diagnosis of bancroftian filariasis.(1986-04-01) Reddy, M V; Prasad, G B; Harinath, B CItem Melanotic neuroectodermal tumor of infancy.(1989-01-01) Prasad, K R; Bhaskaran, C S; Reddy, S J; Aruna, C A; Prasad, G B; Chandrasekhar, MItem Relationship of C3 & C4 levels with filarial immune complexes in bancroftian filariasis.(1989-09-01) Prasad, G B; Harinath, B CC3 and C4 levels were determined by radial immuno-diffusion technique and filarial circulating immune-complexes by anti-C3 ELISA in microfilaraemic individuals and patients with clinical filariasis. Decreased levels of C3 and C4 were observed in both groups of filarial patients. Low levels of complement components were associated with low levels of circulating immune complexes.Item Suppression of Con A induced lymphocyte transformation by sera from filarial patients with clinical manifestations.(1991-04-01) Prasad, G B; Kharat, I; Harinath, B CReduced lymphocyte transformation to Wuchereria bancrofti microfilariae excretory-secretory antigen and Con A were observed in clinical filarial patients. Pre-incubation of normal human peripheral blood mononuclear cells with sera from filarial patients with clinical manifestations such as hydrocele and elephantiasis suppressed Con A induced responses. Effect of fractionated clinical filarial serum on Con A induced lymphocyte transformation showed that the inhibitory activity was associated with high molecular weight serum fraction.Item Transplacental transfer of filarial infection.(1986-03-01) Agarwal, M; Prasad, G B; Harinath, B C; Bhatia, B D