Browsing by Author "Patel, Rimpal"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Acute Toxicity Study of Vishatindukadi Vati Prepared by two References: Evaluation of Toxic Effects in Wistar Rats
    (Mahadev Publications, 2025-03) Patel, Rimpal; Patel, Kirti
    Acute toxicity studies were conducted to assess the short-term adverse effects of two formulations of Vishatindukadi Vati (VTV1 and VTV2) in rats, as per OECD 423 guidelines. VTV1 contains Kupilu, Maricha, Puga Phala and Chincha Beeja while VTV2 contains Kupilu, Maricha and Bhavana of Nagavalli Patra Svarasa. This study aims to conduct an acute toxicity study of Vishatindukadi Vati, prepared with two references. The experimental procedure involves using female Wistar rats (Rattus norvegicus), aged 8 weeks. Each rat weighs between 200 and 300 grams, with a variation of ±20% of the mean weight. A total of 3 animals are used per set for the study. Mortality occurred in all animals administered VTV1 at 2000mg/kg body weight, whereas no mortality was observed in VTV2 at the same dose. VTV1 exhibited toxicity, potentially due to the high concentration of Kupilu, which contains the neurotoxic alkaloids strychnine and brucine. The Bhavana of Nagavalli Patra in VTV2, known for its antidote properties, may reduce oxidative stress and inflammation, potentially mitigating toxicity. The LD50 of VTV1 was classified under GHS Category 4 (greater than 300mg/kg), while VTV2 fell under GHS Category 5 or Unclassified (greater than 2000mg/kg). This study emphasizes the significance of Shodhana (purification) processes and antidote substances in mitigating the toxicity of formulations.
  • No Thumbnail Available
    Item
    Pharmaceutical Standardization and Preliminary Analysis of Vishatindukadi Vati Prepared by two References
    (Mahadev Publications, 2025-02) Patel, Rimpal; Bharat, Kalsariya
    Standardization of herbo-mineral drugs is essential to certify their quality, purity and reproducibility. Vishatindukadi Vati is a herbo-mineral formulation mentioned in Rasatantrasaara and Siddhaprayoga Sangraha (RTSSPS) with two references and different ingredients. The formulation mentioned in Gutika Prakarana contains Kupilu (Strychnos nux- vomica Linn), Maricha (Piper nigrum Linn.), Puga Phala (Araca catechu Linn.) and Chincha Beeja (Tamarindus indica Linn). while the formulation mentioned in Vatavyadhi Prakarana contains Kupilu (Strychnos nux-vomica Linn.) and Maricha (Piper nigrum Linn.) with Bhavana of Nagavalli (Piper betel Linn.) Patra Svarasa. It is mentioned in context of various diseases i.e., Jirna Vatavyadhi (chronic disorders due to Vata), Jirna Jvara (chronic fever), Ajirna (indigestion), Mandagni (subdued digestive power) and many more. In pharmaceutical process; 3 batches of Kupilu Shodhana and using both references three batches of Vishatindukadi Vati were prepared and its organoleptic and physico-chemical analysis were performed. Organoleptic and physicochemical analysis of raw materials, in-process and finished products were done. In physico-chemical pH, loss on drying at 105°C, total ash (%w/w), acid insoluble ash (%w/w), water-soluble extractive (%w/w), alcohol soluble extractive (%w/w), uniformity of weight (mg), friability (%), hardness (kg/cm2) and disintegration time (min.) were carried out for VTV1 and VTV2. An average value of VTV1 and VTV2 were 5.3, 6.48%, 1.55%, 0.24%, 22.5%, 6.96%, 124.3mg, 0.16%, 4.6kg/cm2, 37.6min and 5.05, 7.24%, 2.39%, 0.26%, 23.7%, 6.69%, 124mg, 0.14%, 3.63kg/cm2 and 32 min. respectively. It needs further preclinical and clinical studies to prove its safety profile and therapeutic efficacy.