Browsing by Author "Panjwani, Poonam"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Assessment of HER-2/neu status in breast cancer using fluorescence in situ hybridization & immunohistochemistry: Experience of a tertiary cancer referral centre in India.(2010-09) Panjwani, Poonam; Epari, Sridhar; Karpate, Arti; Shirsat, Hemlata; Rajsekharan, Preetha; Basak, Ranjan; Shet, Tanuja; Chinoy, Roshni; Chacko, Roy; Gursale, Sampada; Baraskar, Nayana; Gupta, Sudeep; Hawaldar, Rohini; Desai, SangeetaBackground & objective: Determination of HER2 status in breast cancer has become important to identify potential candidates for anti-HER2 therapy. In this study we compared fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) for the determination of HER2 status in breast cancer patients referred to a tertiary care referral centre. Methods: A total of 200 cases of invasive breast cancer were evaluated for HER2 status using IHC and FISH and results were compared. Results: The IHC 3+ (93.9%) and IHC negative (85.9%) cases showed good concordance with the corresponding FISH results; while 66.6 per cent of IHC 2+ cases showed gene amplification by FISH. In addition, hormone receptor expression and HER2 gene status showed a statistically significant inverse association (P<0.05). Interpretation & conclusion: These findings reaffirm IHC as a prudent first-step to screen tissue samples for HER2 status and to determine suitability for technically demanding FISH test and the dual coloured FISH as a gold standard for determination of HER2/neu status in IHC equivocal cases of breast carcinoma.