Browsing by Author "Pandita, Archana"

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    Antibacterial effect of silver and copper nanoparticles derived from Klebsiella pneumoniae-NSB2 strain against pathogenic bacteria
    (The India Publications and Information Directorate (CSIR-NISCAIR), 2025-06) Madhav, Kunal; Pandita, Archana; Rani, Diksha
    The rising threat of multidrug-resistant (MDR) bacteria has escalated the search for alternative antimicrobial solutions, including the use of nanoparticles. This study evaluated antibacterial activity of biosynthesized silver (AgNP) and copper nanoparticles (CuNP), produced by the Klebsiella pneumoniae NSB-2 strain against Escherichia coli and Staphylococcus aureus. UV-Visible spectrophotometry confirmed the synthesis of these nanoparticles, with absorption peaks at 421 nm for AgNPs and 419 nm for CuNPs. TEM analysis revealed spherical nanoparticles with sizes of 29.3 nm for AgNPs and 31.5 nm for CuNPs. AgNPs demonstrated a minimum inhibitory concentration (MIC) of 30 µg/mL for both pathogens, while CuNPs had an MIC of 40 µg/mL for E. coli and 30 µg/mL for S. aureus. Both nanoparticles exhibited bactericidal activity at 40 µg/mL, with AgNPs showing greater antibacterial potency. Growth rate (µ) and doubling time (Td) analyses revealed that bacterial growth slowed, and doubling times increased in the presence of both nanoparticles. Results indicate that AgNPs were more effective at lower concentrations compared to CuNPs, underscoring their stronger impact on bacterial growth kinetics. This study suggests that AgNPs and CuNPs hold promise as alternative treatments for MDR bacterial infections, with AgNPs showing superior efficacy.
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    Corneo- Conjunctival Auto Grafting in Pterygium Surgery
    (2004-07) Sharma, Ashok K; Wali, Vijaya; Pandita, Archana
    To evaluate the efficacy of conjunctival auto grafting in surgical management of primary and recurrent pterygium, the record of 150 patients comprising 47 females and 103 males who underwent corneocunjunctival auto graft transplant surgery for primary and recurrent pterygia from 1997 to 2003 was reviewed retrospectively. Follow up was done for six months after the surgery. Recurrence of pterygium was considered as failure. All grafts were transplanted promptly. Recurrence was noted in four patients (2.6%). Mild discomfort in the immediate post-operative period was noted in all the patients. Donor site healed without any complication in all the patients.The results indicate that corneo-conjunctival autograft transplantation is an effective treatment for management of both primary & recurrent pterygia.
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    Gamma Linolenic Acid in Dry Eye
    (2006-01) Sharma, Ashok K; Raj, Hans; Pandita, Archana; Tandon, Vishal R; Mahajan, Annil
    In this prospective study to evaluate the role of gamma linolenic acid in the management of dry eye, 100 patients of dry eyes were studied by dividing randomly into two groups of 50 each. In the test group, all the 50 patients were put on medication which consisted of cap gamma linolenic acid - 120 mg once daily dose, artificial tears solution/ointments 4-6 times daily with a topical mild steroid (loteprednol etabonate 0.5%) thrice daily. In the control group all the 50 patients were put on artificial tear solution/ointments and topical mild steroid (loteprednol etabonate 0.5%) thrice daily. All the patients were subjected to slit lamp examination, schirmer test, and fluorescien stain for tear film break up time (TBUT). In the test group symptomatic relief was shown by all 50 (100%) patients after one week of treatment. There was not much improvement in the schirmer test and TBUT results at 1 week. At 1 month follow up the result showed improvement and at 6 months there was definite increase in the schirmer test & TBUT values. The results were not influenced by the age & gender of the patients. In the control group though there was definite symptomatic relief in all the patients, but there was no change in the major study parameters i.e. tear film meniscus height; schirmer test and TBUT. At six month of the study there was marginal improvement of the major parameters but there was a lot of difference between the test and control groups. Thus gamma linolenic acid has a definite role in the management of dry eye.