Browsing by Author "Nema, S"
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Item Antibiogram study over bacterial isolates from cases of bacteraemias.(1996-09-01) Nema, S; Chitnis, D SDuring January, 1994 to December, 1994, 703 of the 1778 blood specimen were positive for blood culture and 59.9% of the isolates were of staphylococci. Thirty two percent of the staphylococci were methicillin resistant. Netilmycin showed the lowest in vitro resistance among Gram positive cocci & Klebsiella species. Netilmycin, amikacin, ciprafloxacin and the third generation cephalosporins in general showed greater in vitro sensitivity among gram negative bacilli. The netilmycin had the lowest incidence of drug resistance based on the pooled data over the blood culture.Item Emerging bacterial drug resistance in hospital practice.(1997-08-01) Nema, S; Premchandani, P; Asolkar, M V; Chitnis, D SThe growing multiple drug resistance among bacteria in hospital practice is posing a serious threat to the successful antimicrobial therapy. Our data on the bacterial drug resistance at a tertiary care centre during 1995-1996 has been alarming with an incidence of 73 to 99% resistance to the common antibiotics like ampicillin, chloramphenicol, cotrimoxazole and first generation cephalosporins among the gram negative isolates. The resistance to gentamicin and ciprofloxacin ranged from 53 to 79%. Resistance to amikacin, netilmicin and the third generation cephalosporins ranged from 30 to 73%. The frightening observation was the emergence of resistant isolates which were sensitive only to two drugs, sensitive only to one drug and resistant to all the available antibiotics (2.64, 17.6 and 11.5% respectively) during 1994 to 1996. Resistance among the gram positive bacteria was much less but the increase in methicillin resistant Staphylococci (52-65%) was a serious matter. The data were an eye opener and the infection control measures could bring marginal improvement in the situation in 1996. It is vehemently appealed that the national antibiotic policies be formed and be stringently implemented before we are thrown back to the pre-antibiotic era.Item Study of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing.(2010-10) Mehta, P R; Nema, S; Paranjpe, S; Ingole, N; Wanjare, S; Nataraj, GAims: To determine the prevalent subtypes of HIV-1 in serodiscordant couples. Setting: Integrated Counselling and Testing Centre (ICTC), Department of Microbiology. Study Design: Prospective pilot study. Participants: Thirty HIV-1 serodiscordant couples. Inclusion Criteria: a) Documentation of HIV-1 infection in one partner and seronegative status in the other, current history of continued unprotected sexual activity within the partnership, demonstration that they have been in a partnership for at least 1 year and are not currently on highly active antiretroviral therapy HAART; b) willingness of both partners to provide written informed consent including consent to continued couple counselling for 3 months. Materials and Methods: HIV-1 subtyping was carried out by heteroduplex mobility analysis (HMA) by amplifying env region; and DNA sequencing by amplifying gag region. Results: HIV-1 env gene was amplified successfully in 10/30 samples; gag gene, in 25/30 samples; and both env and gag gene were amplified successfully in 5/30 samples. HIV-1 subtype C was detected from 21 samples; subtype B, from 7; and subtype A, from 2. Sample from 1 positive partner was detected as subtype C by env HMA and subtype B by gag sequencing. Conclusion: HIV-1 subtype C was found to be the predominant subtype of HIV-1 in serodiscordant couples attending our ICTC, followed by HIV-1 subtype B and HIV-1 subtype A, respectively. DNA sequencing was found to be the most reliable method for determining the subtypes of HIV-1.